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Articles by K.L. Tiwari
Total Records ( 10 ) for K.L. Tiwari
  Shubhra Tiwari , S.K. Jadhav , Mayuri Sharma and K.L. Tiwari
  Dwindling supplies of petroleum and growing environmental concerns over its use has led to increasing interest in developing biomass as a feedstock for liquid fuels. In particular, bioethanol produced from biomass represents a promising alternative fuel or gasoline extender. Bioethanol is used in vehicles either as a sole fuel or blended with gasoline. As an oxygenated compound, ethanol provides additional oxygen in combustion and hence obtains better combustion efficiency. The main environmental advantages of fuel ethanol are its sustainability in using a renewable resource as a feedstock, thus promoting independence of fossil fuel and maintaining the level of greenhouse gas. Bioethanol can be produced from carbohydrate containing substrates by the process of fermentation. Many microorganisms like bacteria, fungi and yeast involved in bioethanol production. Present study deals with bioethanol production from rotten fruits with inoculation of bacteria isolated from respective fruits. Rotten fruits serve as potential feedstock for bioethanol production due to high sugar content and cost effective substrate. Results indicate that among 5 fruits rotten sapota (Manilkara zapota) produced highest amount of bioethanol 9.40% on 5th day of incubation. Bioethanol can solve the problem of pollution and considered as fuel for future.
  Neha Behar , K.L. Tiwari and S.K. Jadhav
  Curcuma caesia Roxb. belonging to family Zingiberaceae is an important traditional medicinal plant. The rhizome has been widely used as folklore medicine. Chhattisgarh state medicinal plant board has categorized it as an endangered plant. Turmeric/ginger is vegetatively propagated exclusively through underground rhizomes and multiplication rate is very low. Furthermore, susceptibility to diseases, specifically soft rot caused by Pythium species causes heavy losses. In vitro micropropagation technique can be useful for conservation and commercial exploitation of valuable secondary metabolites in medicinal plants. In vitro plant regeneration system depends upon many factors but the type of explants has been identified as one of the major factor. Thus, the present study was conducted using various parts of the plant viz. leaf, root, rhizome sections, mature bud of rhizome and sprouted bud of rhizome in different hormone concentration and combinations in MS medium. Only mature bud and sprouted bud from the rhizome responded while others did not show any sign of morphogenesis. Sprouted and mature bud showed best response in 4 mg L-1 BAP+100 mg L-1 ADS with mean 3.8±0.32 number of shoots and mean 3.28±0.42 cm length, similarly, 1±0.39 mean shoots and mean 1.17±0.48 cm length in mature bud. In vitro produced plants were easily established in soil with almost 100% survival and were morphologically similar to their parent plants. Thus, sprouted buds of the rhizome can be exploited for further micropropagation and conservation studies.
  Jai Shankar Paul , K.L. Tiwari and S.K. Jadhav
  Preservation of commercial and research important fungi for long time period is a very tedious job. Mostly cryopreservation technique in liquid nitrogen is used for long term preservation. In this work 15 different species of commercial and research important fungi are preserved at 4°C in different concentration of glycerol using two different methods (Slant culture and Slice cut method). In slant culture method at 50% of glycerol, 100 and 86.66% of fungi are viable upto 24 and 30 months of preservation, respectively. In slice cut culture method 100% of fungi are having regeneration capacity upto 24 months of preservation. This study help to preserve the fungi with easy and low cost for long term period at 4°C under refrigerator.
  Pramod Kumar Mahish , K.L. Tiwari and S.K. Jadhav
  Background and Objective: Eco-friendly biological removal of heavy metal pollution from aqueous solution is a recent approach which using the biosorbent. It has lots of advantages over chemical approach like its low cost, more efficient, more effective, multiple uses etc. The fungi are one of the most desirable absorbents because of its cell wall characteristics. The present work, therefore, aimed to use Penicillium oxalicum isolated from lead-contaminated industrial wastewater to remove lead from aqueous solution. Materials and Methods: Its tolerance towards lead metal ions was studied by growing fungus in different metal concentration. Biosorption of lead by live fungal biomass was determined and different parameters like pH, temperature and incubation period were taken to observe maximum absorption. Physically and chemically retreated biomasses of the fungus were also used for lead biosorption. Lead metal concentration was determined by PAR methodology in all procedures. Results: Tolerance index of P. oxalicum was recorded 67.44 and relationship between tolerance by fungus and lead concentration was found R2 = 0.99. The lead metal ion was absorbed by live biomass which is affected by the incubation period, pH and temperature. Maximum 89.82% lead was absorbed by pretreated biomass of fungus. Among the different pretreatment procedure, detergent pretreatment was found most efficient. Conclusion: Penicillium oxalicum biomass is now one alternate to remove lead from aqueous solution. The industrial effluent was found an important source of potential micro-organism.
  Anshika Pandey , Shubhra Tiwari , S.K. Jadhav and K.L. Tiwari
  Bioethanol is a suitable renewable or alternative energy source and also the potential solution of all the problems related with the environment and energy crisis. Today ethanol derived mainly from glucose or starch sources of agriculture stock. The human demand for food, however, has yet to be met. To solve both the energy and food problems, there has been increasing interest and worldwide studies in producing bioethanol from lignocellulosic substrate. To promote bioethanol production and its uses, it is necessary to reduce the production cost by using cheap substrate viz., lignocellulosic Azolla and also to obtain the suitable microorganisms which provide sufficient fermentation yield. Azolla consisting 15.19±1.35% cellulose can be suitable substrate for bioethanol production by providing pretreatment to Azolla to hydrolyze the polysaccharides which released fermentable sugar. For pretreatment of Azolla, biological pretreatment is suitable as it is economic feasible and no toxic product formed during pretreatment. Klebisella oxytoca and Saccharomyces cerevisiae, both microorganism have the fermentation ability and can utilized for fermenting lignocellulosic substrate.
  Pramod Kumar Mahish , K.L. Tiwari and S.K. Jadhav
  Lead is important heavy metal found in different parts of the world and its high concentration releases in the environment due to anthropogenic activities. Industries are major sources of emission of lead in the environment. In present study, lead concentration was studied from coal based iron manufacturing industries, iron casting industry and petrochemical industry. The lead concentrations were studied from May, 2010 to April, 2011. All studied industries release lead concentration higher than the permissible limit of standard. Fungi were isolated from industrial effluent containing more than 1.0 mg L‾1 of lead concentration. Dominant fungi isolated from high concentration of lead were further studied for tolerance against lead nitrate. Total 161 colonies of 37 species were isolated from industrial effluent in which genera of Aspergillus were found dominant. Maximum fungi were obtained from Columbia Petrochemical industry. Diversity of fungi is affected according to season and industries. Some fungi were found in all three season and also in high concentration of lead contaminated waste water. The dominant fungi were found tolerant against high concentration of lead nitrate. Hence, these fungi will utilize as biosorbent for absorption of lead from aqueous solution using ecofriendly method.
  Tikendra Kumar Verma , K.L. Tiwari and S.K. Jadhav
  Objective: In the present study the status of iron concentrations and various physicochemical parameters of the effluents of steel and iron casting industries were assessed. Methodology: The monthly observations showed that the iron concentrations ranged between 0.72-6.89 mg L–1 and physicochemical parameters were observed in the following ranges; pH 1.92-8.13, EC 0.9-8.33 mS cm–1, TDS 488.43-5444 ppm, TSS 30-436.67 mg L–1, turbidity 0.16-81.51 NTU, COD 22.4-147.2 mg L–1, DO 0.2-10.6 mg L–1 and BOD 0.2-7.9 mg L–1. The observations revealed that some of the parameters were out of permissible limits of water quality standards formulated by the CBCB and BIS. Results: Further, results showed the significant positive correlation of iron concentration with EC (r = 0.594), TDS (r = 0.516), DO (r = 0.611) and significant negative correlation with pH (r = -0.818) at p<0.01. Conclusion: In conclusion the industrial effluents may affect the quality of water resources, later may be then hazardous to human health.
  K.L. Tiwari , S.K. Jadhav and S. Gupta
  Medicinal plants utilization and conservation has attracted global attention. Optimization of DNA isolation protocol for genetic characterization of any medicinal plants is the necessary and primary step. So, the standardization of DNA isolation is basic requirement for any further research to be carried out. We report here modified CTAB technique for isolation of genomic DNA from five selected medicinal plants namely Catharanthus roseus, Tridax procumbens, Tinospora cordifolia, Aloe barbadensis and Cissus quadrangularis. The comparison of genomic DNA yield from all the five medicinal plants and their different organs (leaves, nodal stem and meristematic region) was performed. We got good yield of DNA from leaves, nodal stem and meristematic region of these medicinal plants. Genomic DNA isolated by modified CTAB method was pure, the highest level of purity was obtained from Aloe barbadensis was 1.93 from leaves and concentration from Tinospora cordifolia was 833.00 μg mL-1 from nodal stem. Other plants also gave good yield of genomic DNA with purity range between 1.26 to 1.93 and concentration range from 179.00 to 833.00 μg mL-1. All the five selected medicinal plants for this research gave good yield of DNA from the established modified CTAB protocol. From all the organs of these medicinal plants DNA obtained was pure and quantity was also good in all five plants.
  Neha Behar , K.L. Tiwari and S.K. Jadhav
  The traditional system of medicine involves the use of different plant extracts or the bioactive constituents for treating various ailments. It is important to establish and compare the phytochemical profiles of lesser known medicinal plant (C. caesia) with the well-known species of the genus (C. longa) for phytochemical similarities or dissimilarity for further drug formulations in this unexplored plant. Thus, this study was conducted which involved soxhlet extraction from leaves and rhizome of both the plant using methanol and chloroform solvents, phytochemical testing for identification of secondary metabolites and thin layer chromatography profiling for presence of curcuminoids. In Curcuma longa, the methanolic extract of rhizome yielded 39.8% while in Curcuma caesia, yield was 6.4%. In Curcuma caesia alkaloids and tannins were found in addition to other metabolites while they were absent in Curcuma longa. In TLC profiling, the rhizome of both the Curcuma species showed presence of curcuminoids while in leaves only methanolic extract in C. longa showed distinctable bands for curcuminoids and in C. caesia, leaves showed only presence of curcumin while dis-methoxycurcumin and bis-demethoxycurcumin was found absent. Presence of medicinally important bioactive compounds in Curcuma caesia depicts that it has great potential for becoming a future drug.
  Veena Thakur , S.K. Jadhav and K.L. Tiwari
  Increasing demand of energy due to industrialization has resulted into the shortfall of non renewable energy sources. Also the over consumption has raised many serious environmental issues, all these problem has resulted for the search of some alternative renewable energy sources which is eco-friendly and biohydrogen fulfils all these requirements. In the present study rice mill effluent was taken as a substrate and Klebsiella oxytoca ATCC 13182 strain was used for the fermentative production of biohydrogen. Hydrogen production was carried out at 40°C temperature and at 6 pH after optimization. Its quantity was measured by water displacement method. Immobilized bacteria were used for the process. Bacteria were immobilized in different concentrations of sodium alginate containing 0.3% activated charcoal. It was found that at concentration of 2 and 3% of sodium alginate 50.33±0.88 and 52.67±1.45 mL productions were observed, respectively with no significant difference. Therefore, effect of HRT (Hydraulic Retention Time) was studied at these 2 concentrations of sodium alginate. The rate of biohydrogen production was found better at concentration of 2%. Effect of metal ions, iron and cobalt was studied at different concentrations and it was found that maximum of 55.00±0.57 mL of hydrogen was produced at 80 mg L-1 concentration of FeSO4 and cobalt nitrate at a concentration of 80 mg L-1 gave maximum production of 58.33±0.88 mL. Different physicochemical parameters of waste water was analyzed before and after fermentation process.
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