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International Journal of Biological Chemistry
Year: 2015  |  Volume: 9  |  Issue: 2  |  Page No.: 79 - 85

Long Term Preservation of Commercial Important Fungi in Glycerol at 4°C

Jai Shankar Paul, K.L. Tiwari and S.K. Jadhav    

Abstract: Preservation of commercial and research important fungi for long time period is a very tedious job. Mostly cryopreservation technique in liquid nitrogen is used for long term preservation. In this work 15 different species of commercial and research important fungi are preserved at 4°C in different concentration of glycerol using two different methods (Slant culture and Slice cut method). In slant culture method at 50% of glycerol, 100 and 86.66% of fungi are viable upto 24 and 30 months of preservation, respectively. In slice cut culture method 100% of fungi are having regeneration capacity upto 24 months of preservation. This study help to preserve the fungi with easy and low cost for long term period at 4°C under refrigerator.

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