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Research Journal of Medicinal Plants
Year: 2012  |  Volume: 6  |  Issue: 1  |  Page No.: 65 - 73

Modified CTAB Technique for Isolation of DNA from some Medicinal Plants

K.L. Tiwari, S.K. Jadhav and S. Gupta    

Abstract: Medicinal plants utilization and conservation has attracted global attention. Optimization of DNA isolation protocol for genetic characterization of any medicinal plants is the necessary and primary step. So, the standardization of DNA isolation is basic requirement for any further research to be carried out. We report here modified CTAB technique for isolation of genomic DNA from five selected medicinal plants namely Catharanthus roseus, Tridax procumbens, Tinospora cordifolia, Aloe barbadensis and Cissus quadrangularis. The comparison of genomic DNA yield from all the five medicinal plants and their different organs (leaves, nodal stem and meristematic region) was performed. We got good yield of DNA from leaves, nodal stem and meristematic region of these medicinal plants. Genomic DNA isolated by modified CTAB method was pure, the highest level of purity was obtained from Aloe barbadensis was 1.93 from leaves and concentration from Tinospora cordifolia was 833.00 μg mL-1 from nodal stem. Other plants also gave good yield of genomic DNA with purity range between 1.26 to 1.93 and concentration range from 179.00 to 833.00 μg mL-1. All the five selected medicinal plants for this research gave good yield of DNA from the established modified CTAB protocol. From all the organs of these medicinal plants DNA obtained was pure and quantity was also good in all five plants.

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