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Articles by C.S. Hayat
Total Records ( 5 ) for C.S. Hayat
  Masood Akhtar , M. Mazhar Ayaz , C.S. Hayat , Abdul Jabbar , M. A. Hafeez , M. Ashfaque and I. Hussain
  The involvement of secretary immunoglobulin system in the protection of mucous surfaces by locally produced antibodies lead to the demonstration of IgA. IgA in the intestine of immune birds may limit coccidian replication. Enzyme linked immunospot (ELISPOT) assay was employed to detect the mucosal immune response (IgA antibody secreting splenic cells) in chicken vaccinated with egg adapted gametocytes antigen. Twenty to twenty-five % (200,000 to 250,000/106 cells) of chicken splenic cells stimulated with gametocytes antigen were detected as IgA secretors. Results of the ELISPOT revealed that spleen is one of the major source of cells producing IgA in chickens. Such a high number of IgA antibody secreting cells indicate protection in birds vaccinated with gametocytes antigen via oral route against coccidiosis. ELISPOT assay is found to be an advantageous alternative to conventional haemolytic plaque technique, being simpler and of equivalent or even greater sensitivity.
  Masood Akhtar , C.S. Hayat , M. Ashfaque and Iftikhar Hussain
  Single radial hemolysis test for the detection of antibodies against Boophilus microplus was developed. Optimum test conditions were determined on the basis of clear hemolysis and maximum haemolytic zone diameter by using human, sheep and rabbit erythrocytes; complement dilutions and temperature. There was a non significant difference (P>0.05) in average haemolytic zone diameters among the erythrocytes of three species used but qualitative analysis indicated that human group "O" erythrocytes at the rate of 2 per cent in agar gel medium gave better results. A 1:2 dilution of complement in vernal buffer at the rate of 8 percent in agar gel medium was found to be optimally appropriate. The combination of over night refrigeration and subsequent incubation at 37 °C in a humid chamber for 8-12 hours gave good results regarding the quality of the zones of hemolysis which were well delineated.
  Masood Akhtar , C.S. Hayat , M. Ashfaque , Iftikhar Hussain , Murad Ali Khan and Sultan Ayaz
  A splenic cell migration inhibition test with modifications was successfully employed in the flat bottom micro titration plates to detect the cell mediated immunity more conveniently and accurately due to vaccine against coccidiosis. A drop of melted agarose (0.2% in PBS) was used to immobilize the splenic fragments. The splenic cells were culture in Hank`s Balanced salt containing fresh chicken plasma and maintained for 10 days. Twenty four hours after cultivation, dominant cell type migrated from a splenic fragment was leukocyte-like cells. At the fourth day these appeared predominantly large round cells and resembled to the macrophage. At the 10th day, spindle cells resembling of fibroblast appeared. The migration of the splenic cells from immunized chickens were inhibited remarkably with the antigen and there was a significant difference (P<0.01) in migration distance of the splenic cells with and without the antigen.
  Masood Akhtar and C.S. Hayat
  Protein subunit (PS) and lipopolysaccharides complex subunit (LPCS) antigens were extracted from the in vitro cultured mid gut cells of Boophilus (B.) microplus. PS and LPCS vaccines with and without adjuvant was prepared and injected in buffaloes. Their efficacy was evaluated on the basis of cellular, humoral and challenge responses. There was a significant difference (P<0.05) in cellular, humoral and challenge responses between the two subunit vaccines. The vaccines with adjuvant gave significantly higher cellular, humoral and challenge responses compared to those without adjuvant. The per cent protection and damage of B. microplus in PS vaccines with and without adjuvant was 75.79 and 61.56; and 44.56 and 42.78 per cent, respectively while in LPCS vaccines with and without adjuvant was 89.25 and 73.79; and 76.25 and 64.16 per cent, respectively. The results of the current study confirm the role of protein and carbohydrates as a protective antigen or as a factor for specificity of these antigen.
  Masood Akhtar , Sultan Ayaz , C.S. Hayat and M. Ashfaque
  Immune response of sonicated coccidiai oocyst was observed in chickens. Indirect haernagglutination (INA) test was developed for detecting antibodies to coccidia and serum antibody levels were measured against soluble oocyst (sporulated) antigen. IHA antibody titre was significantly higher (p<0.05), in chicks vaccinated with inactivated sonicated vaccines as compared to the chicks vaccinated with inactivated sporulated vaccine. The IHA antibody titre vaccinated with inactivated sonicated vaccine ranged from 1 : 64 to 1 : 1:512, among the 15 samples processed, 1:64 in two samples, 1:128 in four samples, 1: 256 in seven samples and 1: 512 in two samples. It gave 100 percent protection to the challenge chicks. Their faeces were normal and no clinical sign was recorded even 40 days post vaccination.
 
 
 
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