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Pakistan Journal of Biological Sciences
Year: 1998  |  Volume: 1  |  Issue: 4  |  Page No.: 389 - 391

Immune Response of Sonicated Coccidial Oocyst in Chickens

Masood Akhtar, Sultan Ayaz, C.S. Hayat and M. Ashfaque    

Abstract: Immune response of sonicated coccidiai oocyst was observed in chickens. Indirect haernagglutination (INA) test was developed for detecting antibodies to coccidia and serum antibody levels were measured against soluble oocyst (sporulated) antigen. IHA antibody titre was significantly higher (p<0.05), in chicks vaccinated with inactivated sonicated vaccines as compared to the chicks vaccinated with inactivated sporulated vaccine. The IHA antibody titre vaccinated with inactivated sonicated vaccine ranged from 1 : 64 to 1 : 1:512, among the 15 samples processed, 1:64 in two samples, 1:128 in four samples, 1: 256 in seven samples and 1: 512 in two samples. It gave 100 percent protection to the challenge chicks. Their faeces were normal and no clinical sign was recorded even 40 days post vaccination.

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