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Pakistan Journal of Biological Sciences
Year: 1999  |  Volume: 2  |  Issue: 2  |  Page No.: 419 - 421

Modified Splenic Cells Migration Inhibition Test For the Detection of Cell Mediated Immunity Against Coccidiosis in Chickens

Masood Akhtar, C.S. Hayat, M. Ashfaque, Iftikhar Hussain, Murad Ali Khan and Sultan Ayaz    

Abstract: A splenic cell migration inhibition test with modifications was successfully employed in the flat bottom micro titration plates to detect the cell mediated immunity more conveniently and accurately due to vaccine against coccidiosis. A drop of melted agarose (0.2% in PBS) was used to immobilize the splenic fragments. The splenic cells were culture in Hank`s Balanced salt containing fresh chicken plasma and maintained for 10 days. Twenty four hours after cultivation, dominant cell type migrated from a splenic fragment was leukocyte-like cells. At the fourth day these appeared predominantly large round cells and resembled to the macrophage. At the 10th day, spindle cells resembling of fibroblast appeared. The migration of the splenic cells from immunized chickens were inhibited remarkably with the antigen and there was a significant difference (P<0.01) in migration distance of the splenic cells with and without the antigen.

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