Abstract: Natural products are always helpful in the maintenance of life and good health. The aim of the present study was to evaluate Luffa acutangula Roxb. var. amara ethanolic seed extract for antioxidant activity by 1,1-Diphenyl-2-picryl hydrazyl and Hydrogen peroxide method, anti-inflammatory by Carrageenan induced rat paw edema method and analgesic activity by tail flick and tail immersion methods. The extract showed potent antioxidant activity i.e., 75.33±0.592 and 76.50±0.281% at 200 μg mL-1 by 1,1-Diphenyl-2-picryl hydrazyl and Hydrogen peroxide method as compared to ascorbic acid. The extract showed significant antiinflammation i.e., 60.8 at 300 mg mL-1 as compared with standard (diclofenac sodium). Further the extract showed significant analgesic activity and the reaction time noted was 6.25±0.52 and 5.80±0.52 at a dose of 400 mg mL-1 as compared to standard (pentazocin). The above results concludes that ethanolic seed extract of Luffa acutangula Roxb. var. amara can be used as natural antioxidant, inflammation and in the treatment of pain.