Abstract: Four flavonoidal compounds identified as quercetin, isorhamnetin-α-3-O rhamnoside, quercetin 3-O-β-D-glucopyranosyl -(1''-6''')-β-D-glucopyranoside and quercetin 3-O-β-D-galactopyranosyl -(6''-1''')- α-L-2''' acetyl rhamnose-(3'''-1'''') β-D-glucopyranoside were for the first time isolated from the ethyl acetate and n-butanol soluble fractions of the alcoholic extract of Fagonia indica Burm F. in addition to oleanolic acid, β-sitosterol-3-O-β-D-glucoside and stigmasterol 3-O-β-D-glucoside. Flavonoids amounted to 3% as estimated colorimetrically by using aluminum chloride. The analgesic activity of the alcoholic extract of Fagonia indica Burm F. was tested by the writhing and the hot-plate tests using acetyl salicylic acid (200 mg kg-1, i.p.,) and morphine (10 mg kg-1, i.p.,) as reference drugs. The cytotoxic and the antimicrobial activities of the alcoholic extract as well as its fractions were also determined. The alcoholic extract elicited activity on both acetic acid-induced writhing response as well as hot plate test in mice showing its central and peripheral mediated antinociceptive action. The alcoholic extract as well as its fractions exhibited marked antitumor activity against the two cell lines tested with the ethyl acetate fraction showing the least IC50 against both carcinoma cells MCF7 and HCT cells while the chloroform fraction showed highest antimicrobial activity against Pseudomonas aeruginosa.