Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2011 | Volume: 7 | Issue: 3 | Page No.: 356-362
DOI: 10.3923/ijp.2011.356.362
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Determination of Antibacterial, Antioxidant and Cytotoxicity Effect of Indigofera tinctoria on Lung Cancer Cell Line NCI-h69

K.P. Renukadevi and S. Suhani Sultana

Abstract:
The present study has been under taken with an objective to determine the antibacterial, antioxidant and cytotoxic activity of the leaf extract Indigofera tinctoria. The selected medicinal plant was collected from near by region of Coimbatore. Antibacterial, activity was carried against gram positive and gram negative bacteria. Antioxidant property was determined both quantitatively and qualitatively. Determination of cytotoxic activity of leaf extract was carried out on lung cancer cell line. The compound present in the extract were identified by GC-MS analysis. The extract screened for photochemical analysis was found to contain bioactive compounds like flavonoids, saponins, tannins, steroidal terpens, phenols and anthroquinone. The leaf extract had shown the ability to inhibit the growth of gram positive bacteria namely Bacillus pumilus, Staphylococcus aureus and Streptococcus pyogenes and zone of inhibition of 16 and 17 mm, respectively but not shown growth of inhibition on gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. Strong antioxidant activity was observed both qualitatively and quantitatively. The strong antioxidant was observed at 250 μg mL-1 with an IC50 value of 51.66 which is higher than that of standard ascorbic acid. The cytotoxic effect of leaf extract on lung cancer cell line NCI-H69 was studied. The percentage cell viability of cells was found to decrease at increasing concentration. GC-MS analysis of the leaf extract shows 6 compounds. This study suggests that ethanol extract of Indigofera tinctoria have profound antibacterial, antioxidant and cytotoxic effect.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Protective Effects of the Phenolic Extracts of Fruits against Oxidative Stress in Human Lung Cells
  •    Antibacterial and Antioxidative Activities of the Various Solvent Extracts of Banana (Musa paradisiaca cv. Mysore) Inflorescences
  •    A Review on the Recent Advances in Pharmacological Studies on Medicinal Plants; Animal Studies are Done but Clinical Studies Needs Completing
  •    Gas Chromatography and Gas Chromatography-mass Spectrometry Analysis of Indonesian Croton tiglium Seeds
  •    The Antiproliferative Activity of Flavanoidal Fraction of Indigofera tinctoria is Through Cell Cycle Arrest and Apoptotic Pathway in A-549 Cells
  •    Enumeration of Antidiabetic Herbal Flora of Tamil Nadu
  •    Chemical Constituents and Biological Activities of Fagonia indica Burm F.
How to cite this article:

K.P. Renukadevi and S. Suhani Sultana, 2011. Determination of Antibacterial, Antioxidant and Cytotoxicity Effect of Indigofera tinctoria on Lung Cancer Cell Line NCI-h69. International Journal of Pharmacology, 7: 356-362.

DOI: 10.3923/ijp.2011.356.362

URL: https://scialert.net/abstract/?doi=ijp.2011.356.362

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

Curve Bottom