Abstract: Nowadays, people's exposure to mycotoxin such was continuously on the rise more and more. Theses compounds had induced an excessive production of free radicals which were responsible for several cell alterations in the organism. Recent investigations had proved the crucial role of nutritional antioxidants to prevent the damage caused by toxic compounds. In this study, we investigated the role of date fruit extract in protection against oxidative damage and distal tubules toxicity induced by chronic exposure to ochratoxin (A) (OTA). The animals were assigned into four groups (n = 8) including control and test groups. Control group received sodium bicarbonate and the animals in the test groups received (289 μg OTA kg-1 b.wt. day-1), (1 mg Ajwa kg-1 b.wt. day-1) and (289 μg OTA kg-1 b.wt. day-1+1 mg Ajwa kg-1 b.wt. day-1), respectively during 28 consecutive days. Oral administration of OTA caused nepherotoxicity as monitored by histological alterations marked by appearance of numerous apoptotic cells with swollen mitochondria and disintegrated membranes. Surprisingly, histopathological examinations showed that date, exerted a protective effect on OTA-induced damages, as revealed by improvement of histopathology changes whereas, in Ajwa date extract+OTA group the severity of the lesions was significantly reduced. In conclusion, this data indicated that OTA, at least partly by interfering in oxidative stress system, exerted its toxic effects on distal tubules whereas in vivo date with antioxidant properties could fairly protected rats against OTA toxic effects and be useful for the prevention of oxidative stress induced nepherotoxicity.