Abstract: Background and Objectives: Characterization and inhibition by probiotics of multidrug resistant (MDR) bacteria causing renal failure patients and receiving hemodialysis were the target of this study. The prime objective of this study was to study the prevalence of MDR within Egyptian renal failure patients and to inhibit them by probiotics. Materials and Methods: The pathogenic bacteria were isolated from clinical samples and were then characterized by biochemical and molecular methods. Inhibition of MDR bacteria by cell free supernatants (CFS) from the probiotic Enterococcus bacium NM2 (E. faecium NM2) was studied in vitro. Results: One hundred bacterial isolates were isolated and into 76% Gram negative bacilli and 24% Gram positive cocci. Based on characterization of such isolates, 7 groups were found and could be arranged in the following descending order according to number of strains identified: Escherichia coli (E. coli, 35%) >Klebsiella pneumoniae (K. pneumoniae, 18%) >Staphylococcus aureus (S. aureus, 17%) >Pseudomonas aeruginosa (P. aeruginosa, 16%) >Proteus vulgaris (Prot. vulgaris, 8%) >Staph. Saprophyticus (4%) >Streptococcus pyogenes (S. pyogenes, 2%). Susceptibility of such bacteria to antibiotics was studied and the more resistant strains (4 strains) were characterized by 16S rRNA cataloging analysis. CFS obtained from the probiotic bacterium E. faecium NM2 inhibited distinctively the growth of 4 MDR bacterial strains (RF22, RF27, RF51, RF55). Conclusion: One hundred bacterial isolates obtained from hemodialysis patients were isolated and identified herein. About 20% of such isolates were MDR. CFS from E. faecium NM2 inhibited the more MDR bacteria.