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Asian Journal of Applied Sciences
  Year: 2014 | Volume: 7 | Issue: 7 | Page No.: 621-634
DOI: 10.3923/ajaps.2014.621.634
 
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Antimicrobial activity of Enterococcus faecium NM2 Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM2

Gamal Enan, Abdul-Raouf Al- Mohammadi, Gamal El- Didamony, Mahmoud E.F. Abdel- Haliem and Azza Zakaria

Abstract:
Cell Free Supernatants (CFS) containing bacteriocin of Enterococcus faecium NM2 (E. faecium NM2) isolated from urine inhibited many gram-positive and gram-negative pathogenic bacteria. It also inhibited the Candida albicans M2 fungus. The antibiotic sensitivity test of the indicator bacteria showed that these strains were resistant to 60-75% of the antibiotics used. The E. faecium NM2 bacteriocin was purified by ammonium sulphate precipitation and gel filtration and a 3600 fold-increase in specific activity of bacteriocin was obtained. The purified bacteriocin showed an apparent molecular mass of Ca, 5 KDa. Amino acid analysis showed that the E. faecium NM2 bacteriocin consists of 16 amino acids with high content of glycine, alanine, glutamic acid and asparagine. The E. faecium NM2 bacteriocin was designated enterocin NM2 and showed a bactericidal action on sensitive bacterial strains used. Enterocin NM2 could be classified as a novel variant within class IIc bacteriocins.
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How to cite this article:

Gamal Enan, Abdul-Raouf Al- Mohammadi, Gamal El- Didamony, Mahmoud E.F. Abdel- Haliem and Azza Zakaria, 2014. Antimicrobial activity of Enterococcus faecium NM2 Isolated from Urine: Purification, Characterization and Bactericidal Action of Enterocin NM2. Asian Journal of Applied Sciences, 7: 621-634.

DOI: 10.3923/ajaps.2014.621.634

URL: https://scialert.net/abstract/?doi=ajaps.2014.621.634

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