Abstract: In this study, initially hydrophobicity of Salmonella enteritidis RITTC 1624 was assessed by Microbial Adhesion to Hydrocarbon Method (MATH). Salmonella enteritidis is an important foodborne enteric pathogen. This organism is highly resistant in environment and remains an important cause of gastroenteritis in human world-wide. Also S. enteritidis forms biofilms on many food contact surface materials. Biofilm formation of bacterium studied at 2, 4, 8, 16 and 20 h after adhesion on surfaces of stainless steel, glass and polyethylene. This organism with 73% hydrophobicity formed biofilm on each of three surfaces but after 2 h biofilm formation was observed on glass and stainless steel more significantly than on polyethylene surface (p<0.05). Results indicated that hydrophobicity of bacteria and surface are important criteria in biofilm formation. The type of surface is also play an important role in biofilm formation of various bacteria. These findings in public health and food industry are very valuable.