Abstract: Nowadays, emergence of bacterial resistance poses a significant clinical problem. Hence, the aim of this study was to describe the current susceptibility patterns of Multi Drugs Resistant (MDR) strains of Urinary Tract Infections (UTI) isolates to current chemotherapeutic agents, as well as to find out antimicrobial characteristics in the extract of seaweeds against MDR. The extract of Padina tetrastromatica, Stocheospermum marginatum and Grateloupia lithophila exhibited strong activity against Non-MDR strains, whereas, the extract of Grateloupia lithophila only exhibited moderate activity against MDR strains. The extract of Caulerpa sp., Gracilaria corticata and Valaniopsis paachanima exhibited week antimicrobial activity. The extract of Grateloupia lithophila inhibited the growth of both MDR and Non-MDR Staphylococcus aureus. Moreover, extract of Grateloupia lithophila inhibited the growth of Non-MDR E. coli, Pseudomonas aeruginosa and Klebsiella. From our findings, the most potent antimicrobial seaweed extract was Grateloupia lithophila which showed maximum inhibitory activity against MDR and Non-MDR Staphylococcus aureus. This study recommends extracts of Grateloupia lithophila can also be used as antibacterial substance for treating multidrug resistant microbes causing acquired infections.