Abstract: A total of 8 samples of cow raw milks were collected from different areas of Khartoum state were analyzed for bacterial load. A total of 23 strains of lactic acid bacteria were isolated, out of which 12 strains were cocci and 11 strains were facultatively heterofermentative lactobacilli. Lactic acid bacteria were identified on the basis of phenotypic characters as Lactococcus lactis, Enterococcus faecalis, Enterococcus faecium, Enterococcus durans, Lactobacillus paracasei subsp. paracasei, Lactobacillus plantarum and Lactobacillus rhamnosus.