Abstract: The antibacterial activity of parsley (Petroselinum crispum) and Prunus mahaleb seed ethanolic extracts were examined using agar disc diffusion method against eleven bacteria (Bacillus anthracis, Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus, Bacillus licheniformis, Brucella melitensis, Escherichia coli,Salmonella typhi, Proteus mirabilis, Bordetella bronshiseptica, Pseudomonas aeruginosa). These extracts had inhibitory effect at various concentrations (0.1, 0.2, 0.3 and 0.4 g mL-1) against Gram-positive and Gram-negative bacteria. Prunus mahaleb ethanolic extract had antibacterial activity against P. mirabilis, B. anthracis and S. aureus. B. licheniformis was the most sensitive organism to the parsley ethanolic extract. Both of the extracts had inhibitory effect against Br. melitensis, E. coli and B. licheniformis in low concentrations (0.1 and 0.2 g mL-1). Based on the results of this study, both plants could be considered as disinfectants or antiseptics, thus confirming their use in folk medicine.