Abstract: The objective of the present study was to investigate the effect of camel milk on activities and gene expression of hepatic antioxidant enzymes in rats intoxicated with carbon tetrachloride (CCl4). Therefore, twenty four rats were used in the current study. Rats were divided into four groups, the first and second groups were received water and camel's milk, respectively whereas rats of both third and fourth groups were injected with CCl4 and received water and camel's milk, respectively. After 5 weeks, liver tissues were collected for biochemical analysis of the activities and gene expression of antioxidant enzymes. Rats supplemented with camel milk alone showed no significant difference in all examined parameters compared to control rats. Liver damage and oxidative stress were evident in untreated CCl4 intoxicated rats as indicated by significant elevation of hepatic enzymes, significant elevation in thiobarbituric acid reactive substance (TBARS), significant reduction in reduced glutathione level (GSH), significant reduction in the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-S transferase (GST) and finally significant down-regulation of antioxidant enzymes gene expression compare to control. Administration of camel milk along with CCl4 caused amelioration in CCl4-induced effects compare to the untreated CCl4 intoxicated rats via up-regulation of antioxidant enzyme gene expression, activation of the expressed genes and increasing the availability of GSH. Conclusively, camel milk exerted its protective effect against CCl4 induced hepatic toxicity by modulating the extent of lipid peroxidation and augmenting antioxidant defense system at activity and gene expression levels.