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Articles by S. Laohasiriwong
Total Records ( 5 ) for S. Laohasiriwong
  N. Sinsiri and S. Laohasiriwong
  This investigation was carried out at the Experimental Farm, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand to evaluate intensity of infection of Pseudocercospora Leaf Spot disease of cowpea cultivars, grown under field conditions with the use of Yasothon soil series (Oxic Paleustults). The experimental design used was a strip split plot design with four replications. The experiment consisted of three factors, i.e., with and without infector rows (Factor A), with and without inoculation and inoculated dates, i.e. none inoculation, inoculated at days 15 and 30 after sowing of seeds in main plots (Factor B) and three cowpea cultivars of KVC7, KKU25 and IT81D-1228-14-1 (Factor C). The results showed that the use of infector rows of cowpea plants (KKU25) being sown at 15 days before sowing seeds of cowpea cultivars in main plots gave a similar intensity of disease as those infector rows being sown at 30 days before sowing seeds of cowpea cultivars in main plots but significantly higher than without infector rows. Pathogenic disease being inoculated to the cowpea plants at 15 days after the sowing of seeds in main plots gave significantly higher intensity of disease than none inoculation. The establishment of infector rows at 15 days ahead of the sowing of seeds in main plots together with an inoculation of the disease at 15 days after the sowing of seeds in main plots gave the best results in evaluating amount of the infected disease. Thus infector rows surrounded the main plots are required whenever trials on disease evaluation are taken place.
  N. Sinsiri and S. Laohasiriwong
  This experiment was carried out in the dry season (February-April, 2004) at the Experimental Farm, Mahasarakham University, Northeast Thailand to investigate the effect of different rates of indole-3-acitic acid (IAA) growth regulator in inducing root formation of detached leaves of MSU1, KVC#7 and IT84E-1-108 cowpea cultivars under tunnel conditions. The experiment was laid in a strip plot design with four replications and each replication was duplicated four times for four sampling periods. IAA levels used were 0, 250, 500, 750, 1,000, 1,500 and 2,000 mg L-1 of distilled water, thus the experiment consisted of 21 treatments. The results showed that root length, number of both roots and root hairs were highly affected by IAA treatments and the best IAA level was found with level 3 (500 mg L-1). The effects due to IAA levels and cultivars were highly significant and the effects due to an interaction between factors A (cultivars) and B (IAA levels), in most cases, were highly significant. Detached leaf technique with the use of IAA growth regulator could be considered as one of the most appropriate techniques for the preparation of plant samples for the test on disease resistance of cowpea cultivars.
  N. Sinsiri and S. Laohasiriwong
  The experiment was carried out at the Experimental Farm, Department of Agricultural Technology, Mahasarakham University, Mahasarakham Province in the 2005 (February to April) for F1 offsprings and also at the Experimental Farm, Khon Kaen University, Khon Kaen Province, Northeast Thailand for F2 offsprings (July to October), i.e., four experiments were carried out, each location had two experiments. The research aimed to investigate inheritance characteristics of F1 and F2 offsprings derived from full diallele crosses of five cowpea cultivars (20 pairs of crosses) against a pathogenic disease of Pseudocercospora cruenta (Sacc,) Deighton. The first two experiments were carried out under field and glasshouse conditions at Mahasarakham University for F1 offsprings and the other two experiments were carried out at Khon Kaen University for F2 offsprings. A Chi square test method was used to justify dominant genes on the infection of the disease. The results showed that the cowpea plants of F1 and F2 offsprings gave only one pair of dominant gene that had its complete dominant effect over recessive gene of the cowpea offsprings.
  A. Poledate , S. Laohasiriwong , P. Jaisil , N. Vorasoot , S. Jogloy , T. Kesmala and A. Patanothai
  The objective of this study was to determine relative importance of gene effects for PBNV incidence and PBNV severity evaluated at 30, 40, 50 and 60 days after planting. Eight generations of three crosses involved three parental lines were evaluated for disease incidence (percentage of infected plants) and disease severity under natural occurrence of PBNV infection in a randomized complete block design with six replications. Evaluations were carried out at 30, 40, 50 and 60 Days After Planting (DAP). The analysis followed Hayman’s model and Gamble’s notations were used to describe parameters of gene effects. Joint scaling test was used to determine adequacy of the model. Additive gene effect was the most important contribution to genetic variation in generation means for both disease incidence and disease severity in the cross ICGV 86388 x IC 10. Selection for lower disease incidence and disease severity in this cross is promising. Additive gene effect and additive x additive epistatic gene effect were also important but in lower magnitude in the cross ICGV 86388 x KK 60-1 for disease incidence at 60 DAP. The presence of significant dominance gene effect in this cross for disease incidence might hinder the progress from selection. The consistent and significant additive gene effect for disease severity might provide a better selection strategy. Additive gene effect was significant for disease incidence only in the cross IC 10 x KK 60-1 at 60 DAP. Additive x dominance epistatic gene effect was also significant at 40 DAP, but no genetic parameter was significant for disease severity. This cross is considered less promising.
  N. Sinsiri and S. Laohasiriwong
  This glasshouse experiment was carried out in the 2004 at the Department of Agricultural Production, Faculty of Technology, Mahasarakham University, Mahasarakham 4400, Northeast Thailand to compare amounts of infection of Pseudocercospora leaf spot disease in leaves of cowpea cultivars being screened between detached leaf and plastic growth pouches techniques. The experiment was laid in a factorial arranged in a Randomized Complete Block Design (RCBD) with four replications. KVC7, IT81D-1228-14-1 and KKU25 cowpea cultivars were used as factor A, detached leaf and plastic growth pouches techniques were used as factor B and four levels of the concentrations of the spores of the disease, i.e., 0, 1x104, 5x104 and 1x105 of disease forming units mL-1 of distilled water were used as factor C. The results showed that KVC7 and IT81D-1228-14-1 cultivars possessed immune and high resistant properties against the disease, respectively, whereas KKU25 cultivar ranked as a susceptible cultivar. Detached leaf technique gave higher amount of infection of the disease than plastic growth pouches technique and the differences were highly significant. A plentiful amount of infected disease was attained with an inoculation rate of 1x105 forming units mL-1 of distilled water, which was considered too high and the most appropriate concentrations for use lie on both concentrations, i.e., 1x104 and 5x104 forming units mL-1 of distilled water. An interaction among factors AxBxC was severely found with KKU25 cultivar, particularly on day 28 after inoculation, whilst other two cultivars gave a minute amount of disease incidences (%) where detached leaf technique gave much higher disease incidences than plastic growth pouches technique, thus detached leaf technique should be of higher advantages.
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