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Pakistan Journal of Biological Sciences
Year: 2007  |  Volume: 10  |  Issue: 1  |  Page No.: 65 - 71

Effects of Different Rates of Indole-3-Acitic Acid on Root Formation of Detached Leaves of Cowpea (Vigna unguiculata L. Walp)

N. Sinsiri and S. Laohasiriwong    

Abstract: This experiment was carried out in the dry season (February-April, 2004) at the Experimental Farm, Mahasarakham University, Northeast Thailand to investigate the effect of different rates of indole-3-acitic acid (IAA) growth regulator in inducing root formation of detached leaves of MSU1, KVC#7 and IT84E-1-108 cowpea cultivars under tunnel conditions. The experiment was laid in a strip plot design with four replications and each replication was duplicated four times for four sampling periods. IAA levels used were 0, 250, 500, 750, 1,000, 1,500 and 2,000 mg L-1 of distilled water, thus the experiment consisted of 21 treatments. The results showed that root length, number of both roots and root hairs were highly affected by IAA treatments and the best IAA level was found with level 3 (500 mg L-1). The effects due to IAA levels and cultivars were highly significant and the effects due to an interaction between factors A (cultivars) and B (IAA levels), in most cases, were highly significant. Detached leaf technique with the use of IAA growth regulator could be considered as one of the most appropriate techniques for the preparation of plant samples for the test on disease resistance of cowpea cultivars.

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