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Articles by Rui Wang
Total Records ( 11 ) for Rui Wang
  Jun Wang , Rui Wang and Xiao-Ye Yang
  For oral application biocontrol of animal parasitic nematode in ruminants, ion beam implantation nematode-trapping fungi must has the capacity to survive the passage through the digestive tract and be efficient in reducing infective larvae of nematodes in the faeces. Ion beam induced mutation the spores of Arthrobotrys oligospora of nematode-trapping. The mutant of the genetic stability is breeded. The fungi were cultured in bottles with corn kernels as a growth media and spores of different doses was respectively administered orally to each group of sheep naturally infected with gastrointestinal nematodes. The control group did not receive fungi. The faeces of these experimental animals were colleted and fecal cultivations carried out. Tested the fungal germination, growth, reproduction and predation livestock parasitic nematode larvae in laboratory. Test the efficacy of a Arthrobotrys oligospora N mutant in nematode-trapping larvae after passage through the digestive tract of sheep. In this research, these capacities were evaluated. The results indicated that ion beam implantation nematode-trapping fungi is a positive mutation. Mutant spores through the digestive tract of sheep can kill livestock parasite nematode larvae in vitro. These results indicate the potential of Arthrobotrys oligospora N mutant as a biological control agent for sheep nematodes. This study showed that such biotechnology can be explored for improving the effectiveness of the use of fungal infections to control livestock parasitic nematodes. This research represents the first application of nematode-trapping fungi in eukaryotic microorganisms.
  Bo Liu , Zheng Qin , Rui Wang , You-Bing Gao and Li-Ping Shao
  The aim of this study is to solve the target assignment of coordinated distributed multi-agent systems. Earlier methods (e.g., neural network, genetic algorithm, ant colony algorithm, particle swarm optimization and auction algorithm) used to address this problem have proved to be either too slow or not stable as far as converging to the global optimum is concerned. To address this problem, a new algorithm is proposed which combines heuristic ant colony system and decentralized cooperative auction. Based on ant colony system, the decentralized cooperative auction is used to construct ants` original solutions which can reduce the numbers of blind search and then the original solutions are improved by heuristic approach to increase the system stability. The performance of the new algorithm is studied on air combat scenarios. Simulation experiment results show present method can converge to the global optimum more stably and faster by comparing the original methods.
  Bin Zeng , Lu Yao and Rui Wang
  For energy efficient routing scheme in the wireless sensor networks, it is important to use sub-optimal paths supported by relay nodes to increase the survivability and lifetime of networks. However, through routing algorithms play an important role, they will not be able to make good performance without an appropriate deployment of relays. First, the co-design problem, relay routing problem and relay deployment problem are formulated together with the end to end network energy consumption. Second, an optimal routing Tree algorithm considering network traffic, are proposed in order to significantly reduce the network energy consumption through the efficient utilization of the deployed relay nodes. Third, the relay deployment problem is considered to find the optimal locations for a given number. A traffic-aware relay deployment algorithm is proposed by adopting the network routing and traffic information. Our algorithms has been evaluated through a series of simulations and compared with state-of-the-art approaches. The results show that they lead to significant improvement on the network energy consumption over the existing traffic-unaware strategies.
  Wentao Sun , ChunLing Lei , Sihai Zhao , LiLun Wang , ChunChao Bi , Rui Wang , XiaoLiang Zhou , Enqi Liu and MingXia Chen
  Observation on morphological and ultrastructural changes of retinopathy in diabetic rabbit long been fed with high sucrose and fat fodders. Use alloxan to establish diabetic rabbit model. Feed the rabbits with high sucrose and fat fodders conduct a Fluorescein Fundus Angiography test (FFA) every 3~4 months to observe morphological changes in retina. Conduct Transmission Electron Microscopy (TEM) examination on eyeball of the model 10~12 months later so as to get wise to the ultrastructural changes in the rabbit retina. The FFA indicated that with the protraction of the disease, Fluorescein Isothiocyanate (FITC) leakage and neo-vasulariztion shadows similar to that of human were seen in the diabetic rabbit model. TEM examination showed that no abnormality was seen in the ultrastructure of the rabbit retina of normal group. The retina of the diabetic rabbit exhibited microfilament and focal dissolving, myelin sheath structure loosening and partial demyelination changes, pericyte mitochondrion tumefaction, endothelial cell layering, space increasing, mitochondrion tumefaction on the nerve fiber layer. Glycogenosome accumulation was seen in the outer nuclear layer retina cells.
  Jinlong Huo , Pei Wang , Jing Leng , Hailong Huo , Lixian Liu , Yue Zhao , Rui Wang and Yangzhi Zeng
  The complete CDS sequence of Banna Mini-pig Inbred Line (BMI) gene STXBP6 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the rat, mouse or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and located on Chromosome 7 of pig and assigned to accession number JF750402. Sequence analysis revealed that the BMI STXBP6 encodes a protein of 210 amino acids that has high homology with the Syntaxin Binding Protein 6 (STXBP6) of four other species rat (100%), mouse (99%), cattle (99%) and human (99%). The phylogenetic tree analysis revealed BMI STXBP6 has a closer genetic relationship with the rat STXBP6 than with those of mouse, cattle and human. Analysis by RT-PCR showed that BMI STXBP6 gene was over-expressed in lung, moderately expressed in midbrain, ovary, diencephalon, spleen, nerve fiber, stomach, small intestine, large intestine, skin, muscle and fat, weakly expressed in kidney and heart and almost not expressed in lymph node, cerebrum, liver and pancreas. The experiment will establish a foundation for further insight into this swine gene.
  Rui Wang , Shan Cong , Ming Cang , Yuzhen Ma , Jianxun Wen and Dongjun Liu
  Bovine ES cells have been isolated but validated bovine ES cell lines have not yet been successfully established. Here, researchers investigated the factors that affect the derivation, proliferation and maintenance of bovine Embryonic Cell (bESC)-like cells including type of feeders (Mouse Embryonic Fibroblasts (MEFs) vs. Bovine Embryonic Fibroblasts (BEFs)), feeder density and inner cell mass isolation procedure. We found that the MEFs work better than BEFs on the process and best at a cell density of 1.25x105 cells/cm2, naturally hatched blastocysts had a short adherence time and high rate of adherence. They were more conducive to bESC-like cell isolation and culture. Culture medium supplemented with IGF promoted proliferation of bESC-like cells but was unfavorable for the maintenance of pluripotency. The findings provide information on the establishment of culture systems for bESC-like cells.
  Li Qiu , Chao Sun , Hu Yang , Chunli Li , Zhang Feng , Yahui Zhao , Xiaoye Liu , Rui Wang , Min Duan , Xinglong Wang and Zengqi Yang
  This study aims to establish a rapid and sensitive multiplex PCR Method for Salmonella detection and the differentiation of virulent strains. Four sets of primers that target hisJ, spvR and fliC were designed and the PCR reaction conditions were then optimized. Based on the detection of reference Salmonella strains and other reference bacterial genera, the PCR Method was able to detect hisJ, spvR, fliC-c and fliC-i in Salmonella. The minimum detectable DNA concentration of the method is 100 pg. The results using the reference strains were consistent with previous research. In addition, this method can also be used in identifying S. typhimurium and S. arizona. Researchers successfully developed a convenient and useful multiplex PCR Method for detecting Salmonella and monitoring virulent strains.
  Guo-Fang Zhang , Yong Wang , Mei-Yu Cai , Da-Min Dai , Ke Yan , An-Shan Ma , Peng Chen , Rui Wang , Ping Li , Jian-Hua Yi , Feng-Qi Zhao , Ji-Zhen Li and Xue-Zhong Fan
  Eight alkaline and alkaline-earth compounds derived from 2,4-dinitroimidazole (2,4-HDNI) were synthesized and characterized by elemental analysis, FT-IR, and TG-DSC, of which K(2,4-DNI) (1), Rb(2,4-DNI)(2,4-HDNI)(H2O)2 (2), and Ba(2,4-DNI)2(H2O)4 (3) were characterized by single-crystal X-ray diffraction analysis. All the three compounds were crystallized from water, but 1 was an anhydrate. The coordination number in 1 and 3 is 10, but in 2 is 9. The 2,4-DNI adopts either η3, η2, or η1 coordination modes depending on the metal cation. In 3, there is an inversion center located on the metal. Only in 2 does water play an important role for the construction of the structure; in 3 it is a hydrogen bonding participant. TG-DSC analyses of 1 were also performed, and non-isothermal decomposition reaction kinetics were obtained.
  Wei Li , Ya-Hu Gao , Min Chang , Ya-Li Peng , Jia Yao , Ren-Wen Han and Rui Wang
  Neuropeptide S (NPS), a recently identified bioactive peptide, was reported to regulate arousal, anxiety, motoring and feeding behaviors. NPS precursor and NPS receptor mRNA were found in the amygdala, the ventral tegmental area (VTA) and the substantia nigra, the area thought to modulate rewarding properties of drugs. In the present study, we examined the influence of NPS on the rewarding action of morphine, using the unbiased conditioned place preference (CPP) paradigm. Morphine (1, 3 and 6nmol, i.c.v.) induced a significant place preference. For testing the effect of NPS on the acquisition of morphine CPP, mice were given the combination of NPS and morphine on the conditioning days, and without drug treatment on the followed test day. To study the effect of NPS on the expression of morphine CPP, mice received the treatment of saline/morphine on the conditioning days, and NPS on the test day, 15min before the placement in the CPP apparatus. Our results showed that NPS (0.3–10nmol) alone neither induced place preference nor aversion, however, NPS (1 and 3nmol) blocked the acquisition of CPP induced by 3nmol morphine, and acquisition of 6nmol morphine-induced CPP was also reduced by NPS (6 and 10nmol). Moreover, the expression of CPP induced by 6nmol morphine was also inhibited by NPS (0.1, 1 and 10nmol). These results revealed the involvement of NPS in rewarding activities of morphine, and demonstrated the interaction between NPS system and opioid system for the first time.
  Ren-Wen Han , Min Chang , Ya-Li Peng , Lian-yong Qiao , Xin-Qiang Yin , Wei Li and Rui Wang

Neuropeptide S (NPS), the endogenous ligand of NPS receptor (NPSR), regulates many biological functions, including arousal, anxiety, locomotion and food intake. NPSR mRNA is expressed in several regions of central autonomic network through which the brain controls visceromotor and other responses essential for survival. However, the role of NPS/NPSR system in regulating gastrointestinal motor is still unknown. Here, we studied the effects of NPS on distal colonic transit in mice. Intracerebroventricular (i.c.v.) injection of NPS (1–1000 pmol) inhibited fecal pellet output and bead expulsion in a dose-dependent manner. However, intraperitoneal injection of NPS (1000 and 10 000 pmol) did not affect fecal pellet output and bead expulsion. In vitro, NPS (0.1–10 μM) also did not modulate distal colonic contractions. Furthermore, i.c.v. co-administration of [D-Val5]NPS, a pure and potent NPSR antagonist, dose-dependently antagonized the inhibitory effects of NPS on fecal pellet output and bead expulsion. In conclusion, our results firstly indicate that central NPS inhibits distal colonic transit through the activation of central NPSR, which implicate that NPS/NPSR system might be a new target to treat function disorder of distal colon.

  Kai Li , Alex Casta , Rui Wang , Enerlyn Lozada , Wei Fan , Susan Kane , Qingyuan Ge , Wei Gu , David Orren and Jianyuan Luo
  Werner syndrome is an autosomal recessive disorder associated with premature aging and cancer predisposition caused by mutations of the WRN gene. WRN is a member of the RecQ DNA helicase family with functions in maintaining genome stability. Sir2, an NAD-dependent histone deacetylase, has been proven to extend life span in yeast and Caenorhabditis elegans. Mammalian Sir2 (SIRT1) has also been found to regulate premature cellular senescence induced by the tumor suppressors PML and p53. SIRT1 plays an important role in cell survival promoted by calorie restriction. Here we show that SIRT1 interacts with WRN both in vitro and in vivo; this interaction is enhanced after DNA damage. WRN can be acetylated by acetyltransferase CBP/p300, and SIRT1 can deacetylate WRN both in vitro and in vivo. WRN acetylation decreases its helicase and exonuclease activities, and SIRT1 can reverse this effect. WRN acetylation alters its nuclear distribution. Down-regulation of SIRT1 reduces WRN translocation from nucleoplasm to nucleoli after DNA damage. These results suggest that SIRT1 regulates WRN-mediated cellular responses to DNA damage through deacetylation of WRN.
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