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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 12  |  Page No.: 2048 - 2053

Molecular Cloning and Characterization of a Novel Gene STXBP6 from Chinese Banna Mini-Pig Inbred Line (BMI)

Jinlong Huo, Pei Wang, Jing Leng, Hailong Huo, Lixian Liu, Yue Zhao, Rui Wang and Yangzhi Zeng    

Abstract: The complete CDS sequence of Banna Mini-pig Inbred Line (BMI) gene STXBP6 was amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the conserved sequence information of the rat, mouse or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and located on Chromosome 7 of pig and assigned to accession number JF750402. Sequence analysis revealed that the BMI STXBP6 encodes a protein of 210 amino acids that has high homology with the Syntaxin Binding Protein 6 (STXBP6) of four other species rat (100%), mouse (99%), cattle (99%) and human (99%). The phylogenetic tree analysis revealed BMI STXBP6 has a closer genetic relationship with the rat STXBP6 than with those of mouse, cattle and human. Analysis by RT-PCR showed that BMI STXBP6 gene was over-expressed in lung, moderately expressed in midbrain, ovary, diencephalon, spleen, nerve fiber, stomach, small intestine, large intestine, skin, muscle and fat, weakly expressed in kidney and heart and almost not expressed in lymph node, cerebrum, liver and pancreas. The experiment will establish a foundation for further insight into this swine gene.

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