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by
O. Baatartsogt |
Total Records (
4 ) for
O. Baatartsogt |
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H.K. Lim
,
K. Choi
,
P.K. Mandal
,
O. Baatartsogt
,
C.H. Lee
,
J.H. Lee
and
H.B. Kim
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This study was carried out to investigate the differentially
expressed genome between S. gallinarum infected and uninfected
control in the spleen lymphocytes of Ross broiler chicks using microarray
analysis. GeneChip Chicken Genome Array containing 32,773 transcripts
corresponding to over 28,000 chicken genes for simultaneous expression
was used. The signal intensity of each gene was normalized and expressed
in fold change. A large numbers of genes were found with differential
expression majority of which are still unknown in chicken genome. Thirty
one known genes were found to have differential expression of which, 25
were up-regulated and 5 were down regulated. Majority of the up-regulated
genes belong to immune response system viz., IL8, IL1B, IL10, IL18,
IL17A, IL15, transferrin, IFNg, TLR2, TNFRSF1b, TNFRSF15 and the down
regulated genes were B-FIV, B-LA, SDF1, B-LBI, belonging to MHC-I and
II and CD1d. To validate the expression of these genes RT-PCR was done
using primers of 12 selected genes′ with total mRNA isolated from
spleen lymphocytes which has confirmed the similar pattern of expression
of all the genes as in microarray. The findings in this study have lead
to the identification of novel genes which may be useful in further studies
to understand the patho-physiology of fowl typhoid towards development
of diagnostics and therapeutics. |
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Hyun-Kyung So
,
P.K. Mandal
,
O. Baatartsogt
,
Hee-Kyong Lim
,
Chi-Ho Lee
,
Jun-Heon Lee
and
Kangduk Choi
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To find the alternative for antibiotic this study was carried out to investigate the differentially expressed proteome between Salmonella gallinarum infected and uninfected control in the spleen lymphocytes of ROS broiler chicks fed with Korean mistletoe using proteomic approach. Total four protein spots were detected with differential expression from the chicken spleen lymphocyte in 2DE gels after silver staining. These proteins were characterized by MALDI-TOF MS and MS/MS. Two known proteins were up-regulated viz., Fatty Acid Binding Protein (FABP) and MRP-126 and 2 proteins were down regulated viz., ribosomal protein12, pyruvate kinase. In this experimental fowl typhoid infection in broilers fed with Korean mistletoe through proteomics approach significant differential expression of four proteins were found which appears to be candidate molecules for fowl typhoid. |
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O. Baatartsogt
,
K. Choi
,
P.K. Mandal
,
H.K. Lim
,
J.D. Oh
and
S.H. Chang
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This research was done to study the protein expression
profile of synovial cells of knee using proteome analysis during the development
of experimental Post-Traumatic Arthritis (PTA) in swine. PTA was induced
by transection of the Anterior Cruciate Ligament (ACL) of left knee in
three piglets. Articular cartilage and synovial tissues were obtained
after 0, 2, 5 and 8 weeks for histopathologic examination. After sacrificing
the piglets at 8 weeks, synovial tissues were collected for 2-dimensional
electrophoresis and mass spectrometric analysis. Histopathologic examination
at 8 weeks showed overt chronic inflammation indicating the development
of ETA. Through proteome analyses more than 1,500 protein spots were identified
in which 7 differentially expressed protein spots were observed in ACL-transacted
synovial tissue. Five proteins were down-regulated (cytoskeletal β
actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α and an
unnamed protein product) and two proteins were up-regulated (α-B
crystalline and Smooth Muscle Protein (SMP) 22α). These results showed
that proteins that are related to cellular organization and signal transduction
are down-regulated and those that are related to cell rescue, defense
and stress are up-regulated. Therefore, the proteome analysis of synovial
tissue provided us new candidate molecules which may be useful to understand
the pathogenesis for diagnostic and therapeutic studies of Post Traumatic
Arthritis (PTA). |
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O. Baatartsogt
,
K. Choi
,
P.K. Mandal
,
Hee-kyong Lim
,
Guan-Hao Li
,
Hong-Sik Kong
,
Dae-Hyun Hahm
,
Chi-Ho Lee
and
Jun-Heon Lee
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To get insights into pathological pathways of Post Traumatic
Arthritis (PTA) this study was done on gene expression profile of synovial
cells of knee using gene chip analysis developing experimental post traumatic
arthritis (ETA) in swine. ETA was induced by transection of the Anterior
Cruciate Ligament (ACL) of left knee in 3 piglets. Articular cartilage
and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic
examination. Synovial cells collected after sacrificing the piglet at
8 weeks, were used at 5 passage for gene expression profiling using Affimetrix
Gene Chip. Histopathologic examination showed overt chronic inflammation
indicating the development of ETA. Through genome analyses it was observed
that 87 known genes were up-regulated and 76 known genes were down regulated.
By analyzing, it was found that many genes with differential expression
are related to inflammation, immune response, lipid binding, cell adhesion,
growth activity and muscle development. The present study provided an
insight into the TA related gene expression pattern. The genome analysis
of synovial cells provided us new candidate molecules which may be useful
to understand the pathogenesis of Post Traumatic Arthritis (PTA). The
established porcine model may serve as in vivo disease model for
further research on traumatic arthritis to elucidate molecular pathogenesis. |
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