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Asian Journal of Animal and Veterinary Advances
Year: 2009  |  Volume: 4  |  Issue: 3  |  Page No.: 148 - 159

Biomarkers Identified by Proteomic Study of Spleen Lymphocyte from Broilers Infected with Salmonella gallinarum after Feeding Korean Mistletoe (Viscum album coloratum)

Hyun-Kyung So, P.K. Mandal, O. Baatartsogt, Hee-Kyong Lim, Chi-Ho Lee, Jun-Heon Lee and Kangduk Choi    

Abstract: To find the alternative for antibiotic this study was carried out to investigate the differentially expressed proteome between Salmonella gallinarum infected and uninfected control in the spleen lymphocytes of ROS broiler chicks fed with Korean mistletoe using proteomic approach. Total four protein spots were detected with differential expression from the chicken spleen lymphocyte in 2DE gels after silver staining. These proteins were characterized by MALDI-TOF MS and MS/MS. Two known proteins were up-regulated viz., Fatty Acid Binding Protein (FABP) and MRP-126 and 2 proteins were down regulated viz., ribosomal protein12, pyruvate kinase. In this experimental fowl typhoid infection in broilers fed with Korean mistletoe through proteomics approach significant differential expression of four proteins were found which appears to be candidate molecules for fowl typhoid.

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