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Articles by Li Zhang
Total Records ( 16 ) for Li Zhang
  Li Zhang , Yuzhu Luo , Jiang Hu , Xiu Liu , Shaobin Li and Wei Yan
  The Calpain3 (CAPN3) gene, located within the confidence interval of the Quantitative Trait Loci (QTL) affecting meat tenderness, being regarded as a candidate gene for meat tenderness in cattle. In this study, SNPs in exon 23-intron 23 region of the yak CAPN3 was investigated from 1032 yak with Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP). Three Single-Nucleotide Polymorphisms (SNPs), resulting in three novel alleles were detected in this region, respectively. These variations included one synonymous in exon 23 and two SNPs in intron 23. Allele C was the most common allele (63.18%) in all of yak. These SNPs are the first report in yak CAPN3 and also suggest further analysis is required to explore the relations between variation sites and meat tenderness.
  Zhiqiang Fan and Li Zhang
  Architectural level reliability-based risk analysis is important for finding risky components during the early stage of developing Ship Command and Control Systems (SCCSs). Therefore, an Architecture Modeling Method (AMM) for supporting reliability-based risk analysis of SCCSs is needed. In previous studies, a generic framework was proposed to derive AMMs for large-scale software-intensive systems. The framework has been applied to derive an AMM (named as SAMM) for supporting architecture description during the development process of SCCSs. In this study, based on the framework, an AMM is defined to support reliability-based risk analysis of SCCSs by extending SAMM, named as SAMM4RRA. SAMM4RRA contains one architecture viewpoint and 11 models and an UML/SysML-based architecture description language. An industrial application of SAMM4RRA, along with the subsequent application of the derived SAMM4RRA architecture model (i.e., a deployed SCCS prototype) was conducted to evaluate SAMM4RRA. Results show that SAMM4RRA meets all the requirements for describing the architecture of SCCSs with the purpose of supporting reliability-based risk analysis.
  Li Zhang and Xiangdong Song
  In order to reduce the number of observations to signal when the shifts occurs in the process, this study proposed the Exponentially Weighted Moving Average (EWMA) median control charts with Variable Sampling Size (VSS). Give the distribution function of the median value assume that the distribution of observations from a process is normally distributed. Then using the Markov chain method to establish a model to calculate the average number of observations to signal (ANOS) of median control chart. Finally, compare the ANOS of the new model with that of the conventional fixed sampling size EWMA control chart and VSS EWMA mean control chart. The data showed that, the designed new model can effectively reduce the ANOS when the process is out of control. That is to say, the new model effectively improves the efficiency of the monitoring process, shifts can be discovered in a shorter period of time which make a great significance to the actual production.
  Song Li , Dongmei Ma , Xiaojing Du , Shuhua Zhou , Yali Song and Li Zhang
  Background and objective: Diosgenin is a steroidal sapogenin known for its pharmacological properties including anti-inflammatory effects. This study was aimed to evaluate the in vitro anti-photoaging and anti-inflammatory effects of diosgenin on ultraviolet B (UVB) radiation-induced damage on human dermal fibroblasts. Materials and Methods: The UVB-induced HDF cells were treated with diosgenin at concentrations 5 and 10 μM, respectively. Cytotoxicity induced by UVB radiation on pre-treated and post-treated HDF cells were tested. The levels of pro-inflammatory cytokines in HDF cells were determined using ELISA method. Western blot analysis was performed to determine the expressions of inflammatory mediators NF-kβ and MMPs. The concentrations of reduced glutathione (GSH) and malondialdehyde (MDA) were determined in the cell homogenates. Results: Diosgenin significantly (p<0.01) reduced the cytotoxicity induced by UVB on HDF cells. Pro-inflammatory cytokines and expressions of inflammatory mediators were significantly reversed (p<0.01) by diosgenin in a concentration dependent manner. Diosgenin was able to increase the level of GSH and prevent MDA formations. Conclusion: The results proved that diosgenin has protective effects on HDF cells against UVB-induced inflammation. Therefore, this active compound can be recommended for pharmaceutical formulations against UV induced skin inflammation.
  Lingyang Xu , Hangxing Ren , Xihui Sheng , Li Zhang , Caihong Wei and Lixin Du
  Gene pyramiding aims to design superior trait through selecting and combining favorite target alleles into a single genotype, thus it was advocated for designing breeding programs via selecting and pyramiding optimal combinations of alleles. In this study, we investigated selection for gene pyramiding design given the animal segregating population and the target trait was controlled by major genes. The admixed population was used as the base population. The mating parents were selected via detecting the favorite genes or linked markers and offspring were produced by the discrete recombination of parents. The phenotypic values were predicted by the genotype-phenotype model. Three selection strategies-genotypic selection, simple phenotypic selection and phenotypic selection integrating molecular information were developed. In genotypic selection, we only considered the favorite allele frequencies and base population sizes in admixed population and in phenotypic selection, we used genotype-phenotype model including trait heritability, gene effect and gene interaction effects to predicate phenotypic values. In each generation, we calculated population hamming distance, average superior genotype frequency and average phenotypic value to comprehensively measure the progress of gene pyramiding. The strategy requires minimum generations to gain gene pyramiding were defined as the optimization strategy. Examples were given for four target genes in order to compare the progress of gene pyramiding. The results indicate that gene pyramiding breeding process was greatly affected by the selection strategy. The gene effect and gene interaction effects information affect the selection of optimal genotype combinations and more precise molecular information was needed to guide the design of effective gene pyramiding breeding programs.
  Lingyang Xu , Fuping Zhao , Hangxing Ren , Jian Lu , Li Zhang , Caihong Wei and Lixin Du
  AGPBSim (Animal Gene-Pyramiding Breeding Simulation) is an individual-based, genetical information integrated simulation program. It was developed to investigate gene-pyramiding breeding given base population sizes, initial allele frequencies and selection strategies. The process of gene-pyramiding breeding was measured using population hamming distance, superior genotype frequency and average phenotypic values. AGPBSim is high flexible at various levels: four cross schemes and three selection strategies and trait architecture using various genotype-phenotype models were integrated in gene pyramiding breeding simulation. The GUI design of AGPBSim can facilitate design of gene-pyramiding breeding strategies by performing virtual breeding simulation on this platform.
  Jianzhen Lv , Zijie Wang , Li Zhang , Hai-Lian Wang , Yuande Liu , Chunyang Li , Jiagang Deng , Yi-Wang and Jin-Ku Bao
  Mangiferin, a C-glucosylxanthone (1, 3, 6, 7-tetrahydroxyxanthone-C2-β-D-glucoside) purified from plant sources was shown to have in vitro growth-inhibitory and apoptosis-inducing activity against MCF-7 cells and it also possessed anti-tumor property on MCF-7 xenograft mice in vivo. Mangiferin triggered G2/M phase cell-cycle arrest via down-regulating cdc2-cyclinB1 singling pathway and induced apoptotic cell death through inhibiting PKC-NFκB pathway in human breast carcinoma MCF-7 cells. In addition, mangiferin had anti-cancer effects in vivo and it could decrease the volume and weight of subcutaneous tumor mass obviously as well as expanded lifespan of xenograft mice. With the molecular mechanisms of mangiferin-induced anti-tumor activities were gradually clarified, traditional Chinese medicine would become potential anti-neoplastic drugs in future cancer therapeutics.
  Lei Zhang , Xiao Lv and Li Zhang
  This study presents a characteristic analysis based on stroboscopic mapping modeling method and fixed points theory in current-fed Contactless Power Transfer (CPT) system. Circulation influence of resonant converter caused by bypass diode in the switch tube and rectifier in secondary is taken into account for loss analyzing, so that the main loss of system is analyzed and quantified. Besides, the piecewise linear stroboscopic mapping model of system is established for analyzing the characteristics of soft switching frequency, output voltage and efficiency when load varies. Simulation is conducted to verify the validity of the proposed model to analysis the output characteristic of current-fed CPT systems.
  Li Zhang and Thien-Fah Mah
  Bacteria growing in biofilms are more resistant to antibiotics than their planktonic counterparts. How this transition occurs is unclear, but it is likely there are multiple mechanisms of resistance that act together in order to provide an increased overall level of resistance to the biofilm. We have identified a novel efflux pump in Pseudomonas aeruginosa that is important for biofilm-specific resistance to a subset of antibiotics. Complete deletion of the genes encoding this pump, PA1874 to PA1877 (PA1874-1877) genes, in an P. aeruginosa PA14 background results in an increase in sensitivity to tobramycin, gentamicin, and ciprofloxacin, specifically when this mutant strain is growing in a biofilm. This efflux pump is more highly expressed in biofilm cells than in planktonic cells, providing an explanation for why these genes are important for biofilm but not planktonic resistance to antibiotics. Furthermore, expression of these genes in planktonic cells increases their resistance to antibiotics. We have previously shown that ndvB is important for biofilm-specific resistance (T. F. Mah, B. Pitts, B. Pellock, G. C. Walker, P. S. Stewart, and G. A. O`Toole, Nature 426:306-310, 2003). Our discovery that combining the ndvB mutation with the PA1874-1877 gene deletion results in a mutant strain that is more sensitive to antibiotics than either single mutant strain suggests that ndvB and PA1874-1877 contribute to two different mechanisms of biofilm-specific resistance to antibiotics.
  Jing Sun , Shu-Yi Deng , Li Zhang , Juan He , Long Jiang , Zong-Wan Mao and Liang-Nian Ji
  Two copper(II) complexes with naphthalene ring [Cu(L1)(H2O)](ClO4)2 · H2O (1) or [Cu2(L2)(ClO4)(H2O)3](ClO4)3 · H2O (2) (L1 = 1-[bis(pyridine-2-ylmethyl)aminomethyl]naphthalene and L2 = 1,4-di[bis(pyridine-2-ylmethyl)aminomethyl]naphthalene) were synthesized. Structural characterization of complex 2 by X-ray crystallography showed that the cations form a double-forficiform structure and each Cu(II) ion is bound by an in-plane N3Ow-coordination. Thermal melting curves and fluorescence spectroscopes of complex-DNA binding indicate that both complexes can efficiently interact with calf thymus DNA and that the binding ability of complex 2 is greater than that of complex 1. Viscosity measurements suggest that complex 2 partially intercalates between DNA base pairs via the naphthalene ring, whereas complex 1 most likely interacts with DNA through the electrostatic binding. In the presence of H2O2 and ascorbic acid, dinuclear complex 2 was more efficient than mononuclear complex 1 in cleaving double-stranded circular DNA into linear DNA. The interaction modes between the complexes and DNA were also discussed.
  Ya-Dan Hou , Zhi-Fu Guo , Ying Yi , Hui-Nan Li , Hao-Ge Li , Li-Jing Chen , Hui Ma , Li Zhang , Jing-Wei Lin and Ming Zhong
  We investigated the changes in the concentrations of protein, soluble sugar, proline, malondialdehyde and the antioxidative enzyme activities of superoxide dismutase, peroxidase, catalase of three wheat (Triticum aestivum L.) cultivars, DN1, Mironovskaya 808 (M808) and Chinese Spring (CS) under cold stress and exogenous abscisic acid treatment in the growth chamber. The results on physiological indices suggested that DN1 and M808 were higher than that of CS on cold resistance. We also found that abscisic acid had significant impacts on cold resistance, suggesting that low concentration of abscisic acid could improve cold acclimation of wheat under low temperature and the high concentration could inhibit cold acclimation. Therefore, physiological changes in wheat seedlings are useful to better understand chilling stress responses in plants which promise to improve cold resistance of crops at low temperatures.
  Yi Tang , Huanxiu Li , Zesheng Yan , Jia Lai , Qian Luo and Li Zhang
  The effects of growth regulators, Thidiazuron (TDZ), silver nitrate (AgNO3), triacontanol and glutaminate on adventitious buds induction from anther culture of bitter melon (Momordica charantia L.) were investigated. It was found that triacontanol was advantageous for buds development and adventitious buds differentiated from anther culture of bitter melon first time. On medium supplemented with glutaminate 0.1 mg L-1, protuberant structures differentiated on the surface of callus. AgNO3 and TDZ has no significant effect on promoting adventitious buds formation from anther culture of bitter melon.
  Heng Pan , Cherry Luo , Runsheng Li , Aimin Qiao , Li Zhang , Marjelo Mines , Alfred M. Nyanda , Jingwu Zhang and Guo-Huang Fan
  The chemokine receptor CXCR4-mediated signaling cascades play an important role in cell proliferation and migration, but the underlying mechanisms by which the receptor signaling is regulated remain incompletely understood. Here, we demonstrate that CXCR4 was co-immunoprecipitated with cyclophilin A (CyPA) from the lysate of HEK293 cells stably expressing CXCR4. Although both the glutathione S-transferase-CXCR4 N- and C-terminal fusion proteins were associated with the purified CyPA, truncation of the C-terminal domain of CXCR4 robustly inhibited the receptor co-immunoprecipitation with CyPA in intact cells, thereby suggesting a critical role of the receptor C terminus in this interaction. Ligand stimulation of CXCR4 induced CyPA phosphorylation and nuclear translocation, both of which were inhibited by truncation of the C-terminal domain of CXCR4. CyPA was associated with transportin 1, and knockdown of transportin 1 by RNA interference (RNAi) blocked CXCL12-induced nuclear translocation of CyPA, thereby suggesting a transportin 1-mediated nuclear import of CyPA. CyPA formed a complex with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which underwent nuclear export in response to activation of CXCR4. Interestingly, the CXCR4-mediated nuclear export of hnRNP A2 was blocked by RNAi of CyPA. Moreover, CXCR4-evoked activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was attenuated by CyPA RNAi, by overexpression of a PPIase-deficient mutant of CyPA (CyPA-R55A), and by pretreatment of the immunosuppressive drugs, cyclosporine A and sanglifehrin A. Finally, CXCL12-induced chemotaxis of HEK293 cells stably expressing CXCR4 or Jurkat T cells was inhibited by CyPA RNAi or CsA treatment.
  Ziqing Wang , Li Zhang , Aimin Qiao , Kurt Watson , Jingwu Zhang and Guo-Huang Fan
  Many cancer cells display down-regulated major histocompatibility complex (MHC) class I antigen (MHC-I), which seems to enable them to evade immune surveillance, whereas the underlying mechanisms remain incompletely understood. Here, we demonstrate that ligand (CXCL12) stimulation of CXCR4, a major chemokine receptor expressed in many malignant cancer cells, induced MHC-I heavy chain down-regulation from the cell surface of the human epithelioid carcinoma HeLa cells, the human U251 and U87 glioblastoma cells, the human MDA-MD 231 breast cancer cells, and the human SK-N-BE (2) neuroblastoma cells. Activation of CXCR4 also induced MHC-I down-regulation in human peripheral blood mononuclear cells. The internalized MHC-I heavy chain molecules were partially co-localized with Rab7, a later endosomal marker. Activation of CXCR4 induced ubiquitination of MHC-I heavy chain, and mutation of the C-terminal two lysine residues (Lys-332, Lys-337) on one of the MHC-I alleles, HLA.B7, blocked CXCR4-evoked ubiquitination and down-regulation of HLA.B7. Moreover, purified GST-conjugated CXCR4 C terminus directly associated with the purified His-tagged β2-microglobulin (β2M), and MHC-I heavy chain was co-immunoprecipitated with CXCR4 in a β2M-dependent manner. This interaction appears to be critical for CXCR4-evoked down-regulation of MHC-I heavy chain as evidenced by the data that MHC-I heavy chain down-regulation was inhibited by either truncation of the CXCR4 C terminus or knockdown of β2M. All together, these findings shed new light on the role of CXCR4 in tumor evasion of immune surveillance via inducing MHC-I down-regulation from the cell surface.
  Qiong Wang , Xue Zhang , Li Zhang , Feng He , Guowei Zhang , Milan Jamrich and Theodore G. Wensel
  The dynamics of G protein-mediated signal transduction depend on the two-dimensional diffusion of membrane-bound G proteins and receptors, which has been suggested to be rate-limiting for vertebrate phototransduction, a highly amplified G protein-coupled signaling pathway. Using fluorescence recovery after photobleaching (FRAP), we measured the diffusion of the G protein transducin α-subunit (Gαt) and the G protein-coupled receptor rhodopsin on disk membranes of living rod photoreceptors from transgenic Xenopus laevis. Treatment with either methyl-β-cyclodextrin or filipin III to disrupt cholesterol-containing lipid microdomains dramatically accelerated diffusion of Gαt in its GTP-bound state and of the rhodopsin-Gαβγt complex but not of rhodopsin or inactive GDP-bound Gαβγ. These results imply an activity-dependent sequestration of G proteins into cholesterol-dependent lipid microdomains, which limits diffusion and exclude the majority of free rhodopsin and the free G protein heterotrimer. Our data offer a novel demonstration of lipid microdomains in the internal membranes of living sensory neurons.
  Lin Yang , Li Zhang , Qiuyu Wu and Douglas D. Boyd
  We previously described the novel zinc finger protein ZKSCAN3 as a new "driver" of colon cancer progression. To investigate the underlying mechanism and because the predicted structural features (tandem zinc fingers) are often present in transcription factors, we hypothesized that ZKSCAN3 regulates the expression of a gene(s) favoring tumor progression. We employed unbiased screening to identify a DNA binding motif and candidate downstream genes. Cyclic amplification and selection of targets using a random oligonucleotide library and ZKSCAN3 protein identified KRDGGG as the DNA recognition motif. In expression profiling, 204 genes were induced 2-29-fold, and 76 genes reduced 2-5-fold by ZKSCAN3. To enrich for direct targets, we eliminated genes under-represented (<3) for the ZKSCAN3 binding motif (identified by CAST-ing) in 2 kilobases of regulatory sequence. Up-regulated putative downstream targets included genes contributing to growth (c-Met-related tyrosine kinase (MST1R), MEK2; the guanine nucleotide exchanger RasGRP2, insulin-like growth factor-2, integrin β4), cell migration (MST1R), angiogenesis (vascular endothelial growth factor), and proteolysis (MMP26; cathepsin D; PRSS3 (protease serine 3)). We pursued integrin β4 (induced up to 6-fold) as a candidate target because it promotes breast cancer tumorigenicity and stimulates phosphatidyl 3-kinase implicated in colorectal cancer progression. ZKSCAN3 overexpression/silencing modulated integrin β4 expression, confirming the array analysis. Moreover, ZKSCAN3 bound to the integrin β4 promoter in vitro and in vivo, and the integrin β4-derived ZKSCAN3 motif fused upstream of a tk-Luc reporter conferred ZKSCAN3 sensitivity. Integrin β4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation. We also demonstrate vascular endothelial growth factor as a direct ZKSCAN3 target. Thus, ZKSCAN3 regulates the expression of several genes favoring tumor progression including integrin β4.
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