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Research Journal of Biological Sciences
Year: 2012  |  Volume: 7  |  Issue: 2  |  Page No.: 69 - 72

Additives Promote Adventitious Buds Induction from Anther Culture of Bitter Melon (Momordica charantia L.)

Yi Tang, Huanxiu Li, Zesheng Yan, Jia Lai, Qian Luo and Li Zhang    

Abstract: The effects of growth regulators, Thidiazuron (TDZ), silver nitrate (AgNO3), triacontanol and glutaminate on adventitious buds induction from anther culture of bitter melon (Momordica charantia L.) were investigated. It was found that triacontanol was advantageous for buds development and adventitious buds differentiated from anther culture of bitter melon first time. On medium supplemented with glutaminate 0.1 mg L-1, protuberant structures differentiated on the surface of callus. AgNO3 and TDZ has no significant effect on promoting adventitious buds formation from anther culture of bitter melon.

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