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Articles by I.O. Igwilo
Total Records ( 3 ) for I.O. Igwilo
  I.O. Igwilo , T.J. Ogoke , D.O. Ogbu , S.N. Igwilo , E. Obi and M.S. Abdulsalami
  Moringa oleifera is a multipurpose tree, cultivated in the tropics and sub-tropics for its nutritional and therapeutic properties. The raw matured seeds which have been reported to be used as food and clarifying agent of turbid water caused growth retardation in albino rats and this might have been due to its anti-nutritional contents. Owing to these adverse factors, the effect of soaking the seeds for 30 min and then compounding it as feeds was done. Its effect on growth rate and the level of some biochemical parameters on rats were investigated. The Wistar albino rats were fed for 21 days and their weights measured at 2 days interval. Aspartate and Alanine transaminases, Alkaline phosphatase and total bilirubin levels were assayed using Automated Vitros 350 Chemistry Analyzer. The growth rates of rats fed with the commercial rat pellets, Casilan diet and the processed Moringa seed diet had a range of 80.06±3.54 to 100.98±5.37, 66.70±7.54 to 55.23±7.47 and 52.99±4.15 to 35.47±2.26, respectively. The parameters assayed for the group that received the processed Moringa seed diet are 144.00±16.80 (AST), 41.00±7.05 (ALT), 66.50±8.80 (ALP) and 12.45±1.18 (Total Bilirubin). The one-way ANOVA statistical analysis indicated that there was no significant change in the parameters of all the groups at 95% significance level (p<0.05). Hence, the soaked Moringa oleifera seed did not support growth in the animal subjects and also did not pose a threat to the liver. However, it is better to develop more suitable processing methods to improve the seed’s nutritional capabilities.
  I.O. Igwilo , F.C. Ezeonu , J.O. Ezekwesili-Ofili , S.N. Igwilo , C.I. Nsofor , M.S. Abdulsalami and E. Obi
  The evergreen plant, Moringa oleifera (Lam) has been known to have both medicinal and nutritional properties, thus its wide use in traditional medicine in Africa and Asia. The roots, in particular, have been reported to possess antibiotic, anti-tumour and anti-oxidative activities. This study therefore seeks to determine the levels of the anti-nutritional factors and other proximate analyses in the roots of a local cultivar of Moringa oleifera which might be responsible for such activities. The concentrations of oxalates and phytates in the roots were determined using the methods of Munro and Bassir and Griffith and Thomas, respectively. The methods of Association of Analytical Chemists (AOAC) were used to estimate the amount of tannins, saponins and cyanogenic glycosides while the Technicon sequential Multi-sample amino acid analyzer (TSM) was used determine the amino acid concentration in the roots. Tannins (45 mg 100 g-1) and oxalates (17.08 mg 100 g-1) were present in the roots at higher levels while saponins (4.20 mg 100 g-1), cyanogenic glycosides (2.72 mg 100 g-1) and phytates (0.07 mg 100 g-1) occurred at much lower levels. The roots contained (Mean±Standard Error of mean) %crude lipid (6.33±1.64), %crude proteins (5.02±1.52), %carbohydrates (76.75), %ash (4.97±0.53) and %moisture (6.93±0.58). The roots lacked the water-soluble vitamins pyridoxine, riboflavin and thiamine but contained ascorbic acid (48.13 mg 100 g-1) and niacin (5.83 mg 100 g-1). This study has shown that Moringa oleifera roots are rich in anti-nutritional factors and that is why they are widely used in traditional medicine in Africa, Asia and Americas for its medicinal importance.
  H.A. Ogbunugafor , F.U. Eneh , A.N. Ozumba , M.N. Igwo-Ezikpe , J. Okpuzor , I.O. Igwilo , S.O. Adenekan and O.A. Onyekwelu
  Oil was extracted from Moringa oleifera Lam (Moringaceae) seeds collected from Enugu, South-East Nigeria and evaluate its physico-chemical and antioxidant properties in comparism to palm oil. M. oleifera seeds gave oil yield of 41.47%. Refractive index, melting point (oC) and acid value (mg KOH g-1) of M. oleifera oil were 1.471±0.00, 28±0.00, 3.80±0.28 while palm oil had 1.473±0.00, 31±0.00, 6.20±0.35 respectively. Similarly, iodine (I2 100 g-1), saponification (mg KOH g-1) and peroxide (mMol kg-1) values obtained for M. oleifera oil were 85.30±0.25, 171.90±0.56 and 8.10±0.07 whereas palm oil had 34.70±0.13, 210.50±0.00 and 13.40±0.28 respectively. Total phenol (mg Gallic Acid Equivalent g-1), total flavonoids (mg Rutin Equivalent g-1) and total antioxidant capacity (mg Ascorbic Acid Equivalent g-1) were 40.17±0.01, 18.24±0.01, 37.94±0.02 for M. oleifera oil and 62.32±0.04, 33.13±0.03, 68.27±0.02 for palm oil respectively. M. oleifera oil and palm oil showed a concentration dependent DPPH free radical scavenging and reducing power capabilities. This study has shown that Moringa Oleifera gave high oil yield, which has good antioxidant capacity with potential for industrial, nutritional and health applications, therefore large scale cultivation of this economic plant could be used as poverty alleviation strategy in Nigeria.
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