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Articles by Yasunori Wada
Total Records ( 2 ) for Yasunori Wada
  Ken Onda , Yukiko Emura , Aya Saito , Tooru Ikeyama , Reiichiro Sato , Hideharu Ochiai , Kazuyuki Kaneko , Tsunenori Iriki and Yasunori Wada
  Acute stress following surgical castration of calves is a common animal welfare concern. Administration of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) can reduce post-castration stress but treatments vary in cost and efficacy. In this study, the effectiveness of sodium salicylate in relieving stress during invasive castration of calves was assessed on the basis of changes in serum cortisol levels and was compared with the effectiveness of ketoprofen. Twenty male Holstein calves were divided into 4 groups: a sham-operated untreated group, a castrated untreated group and 2 castrated groups receiving NSAIDs (sodium salicylate or ketoprofen). NSAIDs were injected intravenously immediately before administering local anesthetic. Blood samples were collected at regular time intervals for measurement of cortisol concentrations. Serum cortisol concentrations of the calves that were administered sodium salicylate were lower than those of the calves that were not administered any NSAID at 1, 1.5 and 2 h after castration and higher than those of the calves that were administered ketoprofen at 2 h after castration (p<0.05). Although, the analgesic and anti-inflammatory effects of sodium salicylate are weaker than those of ketoprofen, the use of salicylic acid derivatives in surgical castration is useful from the viewpoint of animal welfare and entails a significantly lower cost.
  Ken Onda , Reiichiro Sato , Nobue Oba , Eriko Hashimoto , Hideharu Ochiai , Sachiko Arai , Kazuhiro Kawai , Kazuyuki Kaneko , Seigo Itoh and Yasunori Wada
  Leptin is secreted mainly from adipocytes, regulates energy metabolism and influences obesity and many other mechanisms. Milk contains higher concentrations of leptin than blood and mammary epithelial cells produce leptin. The present study examines leptin gene expression in the mammary glands of lactating and non-lactating cows using RT-PCR. The amplified PCR products were sequenced. Researchers found that unlike the mouse, the leptin mRNA is not expressed at all in the non-lactating mammary gland and is sometimes not expressed in the lactating mammary gland of cows. Sequencing analysis confirmed two leptin precursors like those of human and mouse. One precursor consisted of 167 amino acids and the other consisted of 166 amino acids with a deletion at glutamine 49. This study suggests that leptin may be associated with proliferation of mammary epithelial cells to prepare subsequent lactation during pregnancy.
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