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Articles by Xiao-Mao Guo
Total Records ( 2 ) for Xiao-Mao Guo
  Ying-He Qin , Wei Wang , Jia Yao , Jian Wang , Xiao-Mao Guo and Song-Jia Lai
  Meat flavor, one of the most important characteristic of meat quality in farm animals is significantly determined by the Inosine Monophosphate Acid (IMP) content. However, the genetic regulation underlying IMP synthesis has largely remained unknown at the present. In this study, researchers cloned the AMPD1 gene in rabbit and investigated the mRNA expression in longissimus dorsi muscle at 40, 70 and 90 days old, respectively using real-time PCR method. The association between mRNA expression and IMP content was subsequently studied among 48 individuals from two breeds of Tianfu black rabbit (n = 24) and Harbin albino rabbit (n = 24). Total 899 bp fragment containing entire coding region was obtained for rabbit AMPD1 gene. There is no significant difference of IMP content between the two breeds rabbits in the three studied ages (p>0.05). The slaughter age has significant effect on the IMP content with the highest value at 90 days old (3.41±0.29) and the lowest values at 40 days old (2.50±0.39). Researchers detected the negative correlation between IMP content and AMPD1 mRNA expression (r = -0.916, p<0.05). The AMPD1 gene have not significant effects on IMP contents. The results suggested the potential function of AMPD1 to determine the IMP content in rabbit which is significant to conduct the MAS for improving the meat quality.
  Wei Wang , Xiao-Mao Guo , Jian Wang and Song-Jia Lai
  Meat products normally contain low omega-3 fatty acids and high ratio of n-6/n-3 fatty acids may contribute to the prevalence of many diseases. In order to obtained in such beef with high levels of omega-3 fatty acids will be more healthy to meet people’s diet, researchers generated the fat-1 transgenic simmental crossbred cattle expressing the Caenorhabditis elegans c fat-1 gene encoding an omega-3 fatty acid desaturase that converts omega-6 to omega-3 fatty acids and the gene is absent in mammals. In this study, researchers successful product the embryo of fat-1 transgenic simmental crossbred cattle using OSM. The results of Western Blotting and fluoroscopic examination have proved that the fat-1 gene has been into genome of clone embryo and translated into proteins which could be used to embryo transfer.
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