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Journal of Animal and Veterinary Advances
Year: 2011  |  Volume: 10  |  Issue: 19  |  Page No.: 2567 - 2570

Correlation Analysis Between mRNA Expression of AMPDI Gene and IMP Contents in Rabbits

Ying-He Qin, Wei Wang, Jia Yao, Jian Wang, Xiao-Mao Guo and Song-Jia Lai    

Abstract: Meat flavor, one of the most important characteristic of meat quality in farm animals is significantly determined by the Inosine Monophosphate Acid (IMP) content. However, the genetic regulation underlying IMP synthesis has largely remained unknown at the present. In this study, researchers cloned the AMPD1 gene in rabbit and investigated the mRNA expression in longissimus dorsi muscle at 40, 70 and 90 days old, respectively using real-time PCR method. The association between mRNA expression and IMP content was subsequently studied among 48 individuals from two breeds of Tianfu black rabbit (n = 24) and Harbin albino rabbit (n = 24). Total 899 bp fragment containing entire coding region was obtained for rabbit AMPD1 gene. There is no significant difference of IMP content between the two breeds rabbits in the three studied ages (p>0.05). The slaughter age has significant effect on the IMP content with the highest value at 90 days old (3.41±0.29) and the lowest values at 40 days old (2.50±0.39). Researchers detected the negative correlation between IMP content and AMPD1 mRNA expression (r = -0.916, p<0.05). The AMPD1 gene have not significant effects on IMP contents. The results suggested the potential function of AMPD1 to determine the IMP content in rabbit which is significant to conduct the MAS for improving the meat quality.

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