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Articles by W.F. Li
Total Records ( 3 ) for W.F. Li
  Q. Huang , Y.L. Li , X. Xu , Y. Huang , Z.W. Cui , D.Y. Yu and W.F. Li
  The aim of this study is to investigate the effects of Bacillus subtilis BS02 on viability and immune responses of RAW 264.7 cells. RAW 264.7 cells were stimulated with and without B. subtilis spores for 12 h in the treatment group and the control group, respectively. Cytotoxic effect of B. subtilis BS02 on macrophages was measured by cell viability and LDH cytotoxicity assay. Immune responses of macrophage elicited by B. subtilis BS02 were analyzed by measuring the activities of Acid Phosphatase (ACP) and Lactate Dehydrogenase (LDH), the production of Nitric Oxide (NO) and inducible Nitric Oxide Synthase (iNOS) and the secretion of inflammatory cytokines. The results showed that after 12 h incubation, B. subtilis BS02 spores had no influence on viability of RAW 264.7 cells; ACP and LDH activities, the production of NO and iNOS, the levels of inflammatory cytokines [Tumor Necrosis Factor-alpha (TNF-α), Interferon-gamma (IFN-γ), Interleukin-1 beta (IL-1β), IL-6, IL-8, IL-10 and IL-12] were significantly higher than that in the control group (p<0.01). These results indicate that B. subtilis BS02 is not only safe for RAW 264.7 cells but also can activate macrophage immune function.
  W.F. Li , Z.W. Cui , I.R. Rajput , Y.L. Li , H.Z. Wu and D.Y. Yu
  The free radical scavenging systems remove most peroxide which shows antioxidantion capacity of body and lactic acid producing bacteria have capacity to support the body in the mechanism. The present study was initiated to investigate the antioxidantion functioning property of Enterococcus faecium 1 (EF1) to Caco-2 cells under oxidative stress condition. The cells were cultured and randomly divided into 4 groups, the control group (T0), the oxidative stress group (T1), Tert-Butyl Hydroquinone (TBHQ) with addition of H2O2 (T2) and EF1 with combination of H2O2 (T3). The results showed that Total Antioxidation Capacity (T-AOC), Catalase (CAT), Superoxide Dismutase (SOD) activities, Glutathione (GSH) contents in the cultured supernatant and SOD activity of the cells lysate at 12 h increased (p<0.05) in T3 as compared to T1. The supernatant of cells cultured at 12 h significantly improved the SOD, GSH-Px activities and GSH contents in T3. While, Anti Superoxide Anion Free Radical (ASAFR), CAT, SOD and Glutathione Peroxidase (GSH-Px) activities (p<0.05) increased in supernatant at 48 h, conversely Malondialdehyde (MDA) contents was significantly reduced (p<0.05). The SOD activity and GSH contents of cells lysates at 48 h showed similarly reduced (p<0.05). The comparative findings of T3 to T2 supernatant and lysate of cells at 48 h showed significant increase in T-AOC, CAT, SOD, GSH-Px activities and GSH contents of supernatant and in lysate POD activity and GSH contents significantly increased. While, decline (p<0.05) was observed in the MDA contents in supernatant and lysates of T3. The findings revealed that Enterococcus faecium 1 could increase the antioxidation functioning activity of Caco-2 cells under oxidative stress condition.
  W.F. Li , B. Deng , Z.W. Cui , L.Q. Fu , N.N. Chen , X.X. Zhou , W.Y. Shen and D.Y. Yu
  The effect of Bacillus additive on immunity and antioxidant activities of grass carp (Ctenopharyngodon idellus) was studied in this experiment. Animals of the control group were fed the basal diet and those of treatment group were fed the basal diet augmented with Bacillus additive at a dose of 105 Colony Forming Units per gram (CFU g-1) feed. Data showed that serum immunity parameters including IgM, complement C3 and Alkaline Phosphatase (AKP) significantly increased (p<0.05) by 30.77, 34.62 and 107.79% in the treatment group. However, there was no significant difference in serum total protein, globulin, albumin, A/G ratio, Lysozyme (LZM), Glutamic Oxaloacetic Transaminase (GOT) and Glutamic Pyruvic Transaminase (GPT) activities. Serum antioxidant parameters such as serum Glutathione (GSH), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GSH-Px) activities significantly (p<0.05) enhanced by 32.76, 41.75, 20.06 and 11.30%, respectively and hepatopancreatic antioxidant parameters including SOD activity and GSH increased significantly (p<0.05) by 33.83 and 25.86%. In addition, GSH-Px and Malondialdehyde (MDA) content in hepatopancreatic decreased by 20.69% (p<0.05) and 25.39% (p<0.05) individually and the anti-superoxide anion (anti-O2¯) activity in hepatopancreatic decreased by 32.21% (p<0.05) in the treatment group. These results indicate that the Bacillus addtive can promote the immunity and antioxidant function of grass carp.
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