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Journal of Animal and Veterinary Advances
Year: 2012  |  Volume: 11  |  Issue: 13  |  Page No.: 2307 - 2312

Effect of Enterococcus faecium 1 (EF1) on Antioxidant Functioning Activity of Caco-2 Cells under Oxidative Stress

W.F. Li, Z.W. Cui, I.R. Rajput, Y.L. Li, H.Z. Wu and D.Y. Yu    

Abstract: The free radical scavenging systems remove most peroxide which shows antioxidantion capacity of body and lactic acid producing bacteria have capacity to support the body in the mechanism. The present study was initiated to investigate the antioxidantion functioning property of Enterococcus faecium 1 (EF1) to Caco-2 cells under oxidative stress condition. The cells were cultured and randomly divided into 4 groups, the control group (T0), the oxidative stress group (T1), Tert-Butyl Hydroquinone (TBHQ) with addition of H2O2 (T2) and EF1 with combination of H2O2 (T3). The results showed that Total Antioxidation Capacity (T-AOC), Catalase (CAT), Superoxide Dismutase (SOD) activities, Glutathione (GSH) contents in the cultured supernatant and SOD activity of the cells lysate at 12 h increased (p<0.05) in T3 as compared to T1. The supernatant of cells cultured at 12 h significantly improved the SOD, GSH-Px activities and GSH contents in T3. While, Anti Superoxide Anion Free Radical (ASAFR), CAT, SOD and Glutathione Peroxidase (GSH-Px) activities (p<0.05) increased in supernatant at 48 h, conversely Malondialdehyde (MDA) contents was significantly reduced (p<0.05). The SOD activity and GSH contents of cells lysates at 48 h showed similarly reduced (p<0.05). The comparative findings of T3 to T2 supernatant and lysate of cells at 48 h showed significant increase in T-AOC, CAT, SOD, GSH-Px activities and GSH contents of supernatant and in lysate POD activity and GSH contents significantly increased. While, decline (p<0.05) was observed in the MDA contents in supernatant and lysates of T3. The findings revealed that Enterococcus faecium 1 could increase the antioxidation functioning activity of Caco-2 cells under oxidative stress condition.

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