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Articles by Tahira Fatima
Total Records ( 2 ) for Tahira Fatima
  Tayyab Husnain , Tahira Fatima , Rafi-ul-Islam and Sheikh Riazuddin
  In order to develop an efficient protocol for inserting novel genes in chickpea plants, conditions of regeneration and transformation of tissues were optimized. In case of in vitro plant regeneration of Cicer arietinum variety 6153, the hypocotyl tissue showed somatic embryogenesis and regenerated shoots in two basal media with addition of NAA and BAP. Different auxins seemed to have different effects on the differentiation and maturation of embryos. The replacement of one auxin NAA with another IAA induced the germination of embryos. Elaborate rooting of regenerated shoots in two basal media containing IBA was obtained with a frequency of 80 percent. Conditions of biolistic transformation were also optimized for transient expression of marker gene using a homemade particle acceleration gun. The beta-glucuronidase gene was introduced into hypocotyl tissue for transient expression and upto 58 percent of hypocotyl showed transient expression. These results provide the basic information for the transformation of chickpea via somatic embryogenesis.
  Khurram Bashir , Muhammad Rafiq , Tahira Fatima , Tayyab Husnain and Sheikh Riazuddin
  In present studies, level of toxicity of hygromycin was optimized for local inbred line of maize (Zea mays L.). The immature embryos were isolated and cultured on N6 media modified with 1.0 mg L-1 2,4-D, 25 mM L-Proline and 100 mg L-1 Casein Hydrolysate and containing different concentrations of hygromycin. These immature embryos were observed for callus formation on working concentrations of 0, 20, 40, 60, 80, 100 and 120 mg L-1 hygromycin. The results showed that 97.3 38.5 and 32.5% calli survived on media containing 0, 20 and 40 mg L-1 hygromycin. Hygromycin concentration of 60 mg L-1 and above was effective for BR-6 as no callus formation was observed at this concentration or the immature embryos were completely dead. It was also observed that although 32.8% calli survived on media containing 40 mg L-1 hygromycin, these calli were slower in growth, smaller in size and pale in color as compared to calli containing (hph) gene on same media. On the basis of these results 40 mg L-1 hygromycin seems suitable for identification of putative transformants.
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