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Pakistan Journal of Biological Sciences

Year: 2000  |  Volume: 3  |  Issue: 5  |  Page No.: 842 - 845

Plant Regeneration and Expression of Beta-glucuronidase Gene in Hypocotyl Tissues of Chickpea (Cicer arietinum L.)

Tayyab Husnain, Tahira Fatima, Rafi-ul-Islam and Sheikh Riazuddin

Abstract

In order to develop an efficient protocol for inserting novel genes in chickpea plants, conditions of regeneration and transformation of tissues were optimized. In case of in vitro plant regeneration of Cicer arietinum variety 6153, the hypocotyl tissue showed somatic embryogenesis and regenerated shoots in two basal media with addition of NAA and BAP. Different auxins seemed to have different effects on the differentiation and maturation of embryos. The replacement of one auxin NAA with another IAA induced the germination of embryos. Elaborate rooting of regenerated shoots in two basal media containing IBA was obtained with a frequency of 80 percent. Conditions of biolistic transformation were also optimized for transient expression of marker gene using a homemade particle acceleration gun. The beta-glucuronidase gene was introduced into hypocotyl tissue for transient expression and upto 58 percent of hypocotyl showed transient expression. These results provide the basic information for the transformation of chickpea via somatic embryogenesis.

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