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Articles by Rusli Ibrahim
Total Records ( 2 ) for Rusli Ibrahim
  Sobri Hussein , Rusli Ibrahim , Anna Ling Pick Kiong , Nor`aini Mohd Fadzillah and Siti Khalijah Daud
  The first successful micropropagation protocol for an important recalcitrant medicinal plant in Southeast Asia, Eurycoma longifolia Jack or Tongkat Ali as known in Malaysia was developed. In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. Only cotyledon explants were able to form embryogenic callus in the presence of 1.0 mg L -1 (w/v) of 2,4-dichlorophenoxyacetic (2,4-D). A higher yield (60%) of embryogenic callus was obtained when the Type 4 method dissected cotyledon explants were cultured in basal MS medium containing 0.5 mg L -1 (w/v) of kinetin and 1.0 mg L -1 (w/v) of 2,4-D. The highest number of somatic embryos (45±2) was observed in the same medium formulation with the addition of 1.0 g L -1 (w/v) activated charcoal. Subsequent transfer of these mature somatic embryos in basal MS media supplemented with 1.0 mg L -1 (w/v) of kinetin produced a 90% of plantlet regeneration. The differences between the embryogenic and non-embryogenic callus were also determined based on the histological studies.
  Sobri Hussein , Rusli Ibrahim and Anna Ling Pick Kiong
  This study reported the first successful adventitious shoots regeneration from the root and stem explants of E. longifolia. Generally, stem explants appeared to have a lower regeneration capability when treated with the cytokinins alone compared to the root explants. In the root explant, the best medium formulation determined was basal Juglans medium (DKW) supplemented with 1.0 mg L-1 of kinetin + 1.0 mg L-1 of zeatin whereas in the stem explants, basal woody plant medium (WPM) enriched with 2.0 mg L-1 of BAP + 2.0 mg L-1 of zeatin was found to be the best medium formulation in increasing the regeneration rate. Stem and root explants that were 2.0 cm distant from one another has been identified as the most suitable position for attaining the maximum percentage of adventitious shoots formation. Rootlets induction of adventitious shoots formed was also achieved and the highest induction rate was attained in basal MS medium supplemented with 0.5 mg L-1 of Indole-3-butyric acid. Acclimatization of the in vitro plantlets produced survived well with no morphological differences from the parent plants after two months of transplantation to the soil.
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