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Asian Journal of Plant Sciences
Year: 2005  |  Volume: 4  |  Issue: 5  |  Page No.: 472 - 485

Micropropagation of Eurycoma longifolia Jack via Formation of Somatic Embryogenesis

Sobri Hussein, Rusli Ibrahim, Anna Ling Pick Kiong, Nor`aini Mohd Fadzillah and Siti Khalijah Daud    

Abstract: The first successful micropropagation protocol for an important recalcitrant medicinal plant in Southeast Asia, Eurycoma longifolia Jack or Tongkat Ali as known in Malaysia was developed. In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. Only cotyledon explants were able to form embryogenic callus in the presence of 1.0 mg L -1 (w/v) of 2,4-dichlorophenoxyacetic (2,4-D). A higher yield (60%) of embryogenic callus was obtained when the Type 4 method dissected cotyledon explants were cultured in basal MS medium containing 0.5 mg L -1 (w/v) of kinetin and 1.0 mg L -1 (w/v) of 2,4-D. The highest number of somatic embryos (45±2) was observed in the same medium formulation with the addition of 1.0 g L -1 (w/v) activated charcoal. Subsequent transfer of these mature somatic embryos in basal MS media supplemented with 1.0 mg L -1 (w/v) of kinetin produced a 90% of plantlet regeneration. The differences between the embryogenic and non-embryogenic callus were also determined based on the histological studies.

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