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Articles by Rasedee Abdullah
Total Records ( 16 ) for Rasedee Abdullah
  Lawan Adamu , Noraniza Mohd Adzahan , Rasedee Abdullah and Bashir Ahmad
  Problem statement: Endurance horses are generally exposed to severe stress during endurance competitions. These horses are however conditioned to cover the long distances of the competitions at moderate speeds. Approach: This study was conducted in Malaysia to determine the post-race hematological, blood electrolyte and biochemical and physical parameters of horses of 40, 80 and 120 km endurance race categories and eliminated and horses that completed the races with good performance. Whole blood, plasma and serum samples were collected after each race. Results: After physical examination 7 (9.72%) 120 km-, 48 (66.67%) 80 km- and 17 (23.61%) 40 km-race horses were eliminated. Eight horses, all from the 80 km-race category completed the race with good performance. The mean heart of the good performance horses (74.2±13.9 beats min-1) was higher than that of the eliminated 40 km- (68.2±14.7 beats min-1), 80 km-race (62.9±9.7 beats min-1) horses. The blood lactate concentration of good performance horses was 9.2±2.2 mmol L-1, which was significantly higher than in the 40 km- (6.0±2.9 mmol L-1), 80 km- (6.7±3.2 mmol L-1) and the 120 km-race (6.4±1.6 mmol L-1) horses. The blood glucose concentration was lower in the good performance horses (1.6±0.9 mmol L-1) than the eliminated 40 km- (5.7±1.9 mmol L-1), 80 km- (4.3±2.4 mmol L-1) and the 120 km-race (5.0±1.4 mmol L-1) horses. Conclusion: The study showed that eliminated horses exhibited poorer glucose utilization than good performance horses, which may have resulted in poor lactate production. Thus the blood lactate and glucose concentrations of horses during training may be used to predict their performance in endurance races.
  Nagi A. Alhaj , Mariana N. Shamsudin , Hana F. Zamri and Rasedee Abdullah
  The antimicrobial activity of N. sativa essential oil obtained by supercritical fluid extraction by carbon dioxide was investigated against Gram Positive and Gram negative strains, isolated from clinical specimens. Best conditions for Black cumin oil extraction are obtained at 400 bar, 40°C and a solvent flow rate of 25 g min-1. The seed extracts were prepared by supercritical fluid extraction method. Filter paper discs impregnated with varying concentrations of N. sativa extract were tested by the disk diffusion method. Methicillin Resistant Staphylococcus Aureus (MRSA) ATCC strain (700968), E. coli ATCC strain (25922), E. coli 0157 ATCC strain (12799), Extended-Spectrum Beta-Lactamase (ESBL) Klebsiella pneumoniae ATCC strain (700603), Carbapenam Resistant acenitobacter Baumanii (CRAB) clinical strain and Vibrio cholerae 01 Ogawa and 0139 Bengal clinical strains were investigated. The inhibition zones of the Mueller Hinton agar in different extract concentrate ion showed that at 25 mg 20 µL-1, 50 mg 20 µL-1 and 100 mg 20 µL-1, the inhibition zones increased accordingly in S. aureu. However, N. sativa was found to be inactive against ESBL producers (E. coli and K. pneumoniae).
  Nagi A. ALHaj , Mariana N. Shamsudin , Norfarrah M. Alipiah , Hana F. Zamri , Ahmad Bustamam , Siddig Ibrahim and Rasedee Abdullah
  Problem statement: Seeds of Nigella sativa L., commonly known as black seed, have been used in traditional medicine by many Asian, Middle Eastern and Far Eastern Countries to treat headache, coughs, abdominal pain, diarrhea, asthma, rheumatism and other diseases. The seeds of this plant are the most extensively studied, both phytochemically and pharmacologically. The aqueous and oil extracts of the seeds have been shown to possess especially nowadays in pharmaceutical antioxidant, anti-inflammatory, anticancer, analgesic, antimicrobial activities and medicinal and cosmetic applications, sanitary, cosmetic, agricultural and food industries. Approach: The aim of this study was to formulate a new delivery system for dermal and cosmetic application by the incorporation of Nigella sativa essential oil into solid lipid nanoparticles SLN. SLN formulations were prepared following the high-pressure homogenization after starring and ultra-trax homognization techniques using hydrogenated palm oil Softisan 154 and N. sativa essential oil as lipid matrix, sorbitol and water as surfactants. The SLN formulation particle size was determined using Photon Correlation System (PCS). Results: The change of particle charge was studied by Zeta Potential (ZP) measurements, while the melting and re-crystallization behavior was studied using Differential Scanning Calorimetry (DSC). Data showed a high physical stability for both formulations at various storage temperatures during 3 months of storage. In particular, average diameter of N. sativa essential oil-loaded SLN did not vary during storage and increased slightly after freeze-drying the SLN dispersions. Conclusion: Therefore, obtained results showed that the studied SLN formulations are suitable carriers in pharmaceutical and cosmetic fields.
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Mohd Syamil Mohd Yusof , Abdul Rahman Omar , Abdul Aziz Saharee , Abd Wahid Haron , Rasedee Abdullah and Mohd Zamri-Saad
  Haemorrhagic Septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and water buffaloes caused by Pasteurella multocida, serotype B:2 in tropical countries. The limitations associated with accurate predictions of mortality, survival levels and the detection of the presence of the organism from various organs of infected animals. Hence, this study used mouse model to evaluate the pattern of mortality and bacterial recovery from organs. Twenty-four mice were randomly divided into two groups. Infected group were inoculated orally with 109 colony forming unit of P. multocida type B, the group 2 were negative controls. The mice were observed for 5 days post-inoculation. At necropsy, visceral organs of dead animals were subjected for the confirmation using Polymerase Chain Reaction (PCR). The results showed that mortality rate was significantly different (p<0.05) between the infected and control groups. Within infected group, highly significant difference (p<0.05) was observed where 12.5% of the mortality rate was recorded within 24 h and 62.5% within 48 h post-infection. The survival rate, in infected group, was found to be around 25%. In diagnosis, P. multocida type B was detected from all organs of animals that did not survive. In contrast, P. multocida type B was neither recovered nor detected from the organs of mice which survived until the end of the experimental period (120 h). The results of this study indicated that manipulation of the organism in experimental animals provided clear information of the incidence of the disease in the field.
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Acute Phase Protein (APP) investigations of serum or plasma following natural or experimental infection frequently reveal substantial alterations in the APPs, several of which are of veterinary importance in the assessment of herd health. The present study of the experimental nature was conducted to evaluate the acute phase protein responses; haptoglobin, Serum Amyloid A (SAA) and serum albumin in relation to infection with Pasteurella multocida type B and its immunogens; lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) in calves. Eight clinically healthy, non-pregnant and non-lactating Brangus cross calves weighing 150±50 kg were used in this study. The calves (n = 8) were divided into 4 groups of 2 calves in each group. The control group was inoculated with sterile Phosphate Buffered Saline (PBS) whereas group 2 were inoculated with wild-type P. multocida type B:2 and group 3 and 4 were inoculated with LPS and OMP respectively. Blood samples were collected via jugular vein-puncture at 3 h intervals for APPs analysis. APPs were quantified by commercially available ELISA methods. Moribund animals were euthanized while the surviving animals were killed after 48 h. The results revealed that there were statistically significant differences (p<0.05) between APPs concentrations throughout the experimental period in challenged groups compared to control groups. Over 7-fold increase was observed in Hp concentrations with mean maximum levels of 1.316±0.558 ng mL-1, 1.521±0.687 ng mL-1 after experimentally induced with OMP and LPS respectively. SAA increased less than 3-fold with mean levels of 2.187±0.880 ng mL-1, 2.421±0.432 ng mL-1, 2.657±0.099 ng mL-1 in almost all challenged groups; Pasteurella Multocida, OMP and LPS respectively. In contrast, for the negative APP, the albumin levels of groups LPS and OMP did not show significant difference (p>0.05) with mean levels of 32.677±1.556 and 36.185±2.239 U L-1, respectively. While P. multocida group (22.193±2.727 U L-1) showed statistically significant difference (p<0.05) than the negative control group (34.233±6.900 U L-1). In conclusion, the findings of this study indicated that APPs; SAA and haptoglobin are sensitive biomarkers to explore host response in relation to Haemorrhagic Septicaemia infections in clinical settings.
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Pasteurella multocida is an opportunistic pathogen with veterinary and human significance. The species diverse and complexity between the types exist with respect to antigenic variation, host predilection and pathogenesis. The aim of the present study was to determine hematological and serum concentration of biochemical values in calves having infected with Pasteurella multocida type B and its immunogens; Lipopolysaccharide (LPS) and Outer Membrane Protein (OMP). A total of eight clinically healthy calves were divided into four groups of 2 calves in each group. Group 1 were inoculated with sterile Phosphate Buffered Saline (PBS). Group 2 were inoculated with wild-type P. multocida type B: 2 while Group 3 and 4 were inoculated with LPS and OMP extracted from Pasteurella multocida type B, respectively. Blood samples were collected for haematological and biochemical analysis. Results indicated significant differences in the mean values of Prothrombin Time (PT), Partial Thromboplastin Time (PTT) and thrombocyte levels in infected groups 2-4 compared to the control group. There was significant decrease in the mean concentration of Alanine Transaminase (ALT) for group 2 (20.607±1.639 U L-1), group 3 (22.067±1.617 U L-1) and group 4 (40.327±8.351 U L-1). Conversely, there was no significant difference in Alkaline Phosphatase (ALP) for group 2 (149.857±17.327 U L-1) but a significant decrease for groups 3 (114.147±20.901 U L-1) and group 4 (128.971±17.068 U L-1). There was no significant differences in the serum levels of Gamma-glutamyltranspeptidase (GGT) and almost all tested electrolytes in infected groups. In conclusion, our results provide evidence in support of using some haematological and biochemical markers for early detection of individuals at risk for wild type of Pasteurlla multocida.
  Roghayeh Abbasalipourkabir , Aref Salehzadeh and Rasedee Abdullah
  Recently more focus has been put to the development of innovative drug-delivery systems that includes liposomes and solid lipid nanoparticles (SLNs). An in vitro study was conducted to determine the effect of solid lipid nanoparticle on the human breast cancer cell lines (MCF-7 and MDA-MB231). The SLNs based on palm oil were prepared using the high pressure homogenization method and were characterized by the particle size and polydispersity index (particle sizer), zeta potential (zetasizer), ultrastructure [transmission electron microscopy (TEM)] and MTT assay and natural red assay. Homogenization of solid lipid nanoparticles at 1000 bar for 20 cycles produced particles with 145.00±3.39 nm in size and zeta potential of -19.50±1.80 mv. The SLNs were generally round and uniform in shape. The cytotoxicity of the components of the SLN formulation was low: SLN with 1% oleyl alcohol displayed no significant cytotoxicity effect on breast cancer cells. In the light of these findings, SLN stabilized with 30% lecithin and 1% oleyl alcohol as nonionic co-surfactant in aqueous phase was found to be safe for cells and the acceptable for the incorporation lipophilic drugs.
  Lawan Adamu , Noraniza Mohd Adzahan , Rasedee Abdullah and Bashir Ahmad
  Metabolic crises are the convoluted and injurious physiological changes observed in eliminated endurance horses during endurance races. Therefore, this study aims to evaluate the changes in biochemical hematological and cytokine in endurance horses with metabolic crises. The 32 endurance horses between the ages of 6-20 years and weighing between 350-450 kg were examined to be clinically healthy pre-ride. The 16 horses were eliminated post-race as a result of metabolic crises. Blood sample were collected at pre and post ride. The blood samples were analyzed for biochemical, hematological and cytokine. The parameters were assessed using one-way analysis of variance. There were significant increases in RBC, Hb and PCV (p<0.0001). There were significant increases in MCV (p<0.0104) and a significant decrease in MCHC (p<0.0039). There were significant increases in WBC segmented neutrophil and monocytes (p<0.0001). Band neutrophil (p<0.0003) and lymphocytes (p<0.0043). There was a significant increase in IL-6 (p<0.0004). Significant decreases were observed in sodium and potassium (p<0.0001) and chloride (p<0.0004) while significant increases were also assessed in total protein, urea, lactate and creatine kinase (p<0.0001) AST (p<0.0049) glucose (p<0.0207) plasma protein and uric acid (p<0.0014). In conclusion metabolic crises are the major causes of eliminations and poor performance in endurance horses. Thus, this study aims to evaluate the biochemical, hematological and cytokine as indicative of metabolic crises and poor performance in endurance horses.
  Lawan Adamu , Noraniza , Rasedee Abdullah and Bashir Ahmad
  Metabolic crisis is the complex and detrimental physiological changes observed in eliminated endurance horses during endurance events. Therefore, this study aims to appraise the alterations in biochemical, hematological and physical parameters of endurance horses with metabolic crisis. About 174 endurance horses between the ages of 6-20 years were examined to be clinically healthy pre-ride and 115 were eliminated post-race as a result of metabolic crisis. Blood sample was collected and physical examinations were conducted at pre and post ride. The blood sample was analyzed for biochemical and hematological parameters. The parameters were assessed using one-way analysis of variance. Metabolic crisis leads to significant increases in heart rate (p<0.0001), capillary refill time (p<0.005) and significant decrease in gut motility (0.006). There were significant increases in RBC, Hb and PCV at (p<0.0001). There were also significant increases in WBC (p<0.0002), band neutrophil (p<0.012), segmented neutrophil (p<0.0001) and monocytes (p<0.011). Significant decreases were seen in sodium (p<0.0196) and chloride (p<0.0001). Significant increases were also assessed in plasma protein (p<0.0001), albumin (p<0.0001), lactate (p<0.0001), creatine kinase (p<0.0033), uric acid (p<0.0017), gamma glutamyl transferase (p<0.0472), urea (p<0.0014), triglyceride (p<0.0041), total protein (p<0.0001), icterus index and bilirubin (p<0.0024). Thus, these changes could be indicative of metabolic crisis and poor performance in endurance horses.
  Abdinasir Yusuf Osman , Faez Firdaus Jesse Abdullah , Abdul Aziz Saharee , Abd Wahid Haron , Jasni Sabri and Rasedee Abdullah
  Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis, a chronic infectious disease of sheep and goats. Despite the growing interest of this organism, little is known about the causality and effect of this organism on haematological and biochemical values. The purpose of this research, therefore was to determine haematological and serum biochemical features in mice inoculated with whole bacterium and exotoxin (PLD) extracted from C. pseudotuberculosis. The study was performed on apparently healthy mice of 2-3 weeks old (n = 64). The mice were divided equally into 3 groups; namely whole bacterium, exotoxin (PLD) and control group. Mice of whole bacterium group were exposed intraperitoneally to 1 mL of the inoculums containing 109 Colony-Forming Unit (CFU)/mL of live C. pseudotuberculosis. Exotoxin group were exposed intraperitoneally with a single dose of exotoxin (PLD) extracted from C. pseudotuberculosis. Mice served as a control group were challenged intraperitoneally to 1 mL of Phosphate-Buffered Saline (PBS), pH 7. Following post-infection, the mice were subjected for blood sample collection using cardiac puncture method for haematological and biochemical analysis. The results of this study revealed that there were significant decrease (p<0.05) in Hb, thrombocytes count and significant increase in WBC, neutrophils, monocytes counts in the infected group. Biochemically, there were highly significant increase (p<0.05) in the mean levels of CK, AST, ALP and ALT. The mean level of albumin in exotoxin group was significantly lower (p<0.05) compared to C. pseudotuberculosis and control groups while globulin level was significantly higher in C. pseuodotuberculosis group after 48 h. In serum electrolytes, mean level of potassium and phosphate were significantly (p<0.05) higher in infected groups compared to control group while there was no significant (p<0.05) difference in the levels of sodium, calcium and chloride. In conclusions, the present study of experimental nature showed that C. pseudotuberculosis and its exotoxin (PLD) cause disturbances in blood factors and electrolytes and pointed out that t hese haematological and biochemical alterations should be taken into account in the context of diagnosis and treatment of valuable infected hosts in order to prevent further consequences.
  Faez Firdaus Jesse Abdullah , Lawan Adamu , Abdinasir Yusuf Osman , Mohd Zamri Saad , Zunita Zakaria , Rasedee Abdullah and Abdul Aziz Saharee
  Haemorrhagic Septicaemia (HS) is a killer disease of cattle and buffalo of economic importance in Asia and Africa. There is insufficient information on the responses of Balb c mice as animal model in respect of immunogens and Acute Phase Proteins (APP) profiles. Therefore, the present study aims to evaluate the acute phase protein profiles in mice associated with the infection of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Two hundred healthy Balb/c mice of 8-10 weeks old were used in this study. They were divided into four equal groups of 50 mice each. Mice of group 1 were inoculated intra-peritoneal with 1.0 mL sterile Phosphate Buffered Saline (PBS) pH 7, group 2 were inoculated with 1.0 mL of 109 colony forming unit (cfu) of P. multocida B: 2. Mice of groups 3 and 4 were inoculated intra-peritoneal with 1.0 mL of LPS and 1.0 mL of OMP, respectively. Acute phase proteins analysis were done using two sites Enzyme Linked Immunoassay (ELISA) highly sensitive test kits. The data was analyzed using SPSS. Haptoglobin concentration increased significantly in group 3 and 4 (p<0.05) following inoculation with immunogens compared to control group. Mice in group 3 and 4 showed significantly (p<0.000) 3 times higher concentrations of SAA and significantly (p<0.037) 1.3 times increased concentrations of SAA, respectively compared to the control group. There was no significant changes in the concentrations of fibrinogen in group 2 (p = 0.177), group 3 (p = 0.088) and group 4 (p = 0.359). C-reactive protein in groups 2 and 3 showed significantly (p<0.05) higher levels than the control group. Albumin showed significant increase (p<0.05) in group 2 compared to the control group. There were significant changes in the concentrations of acute phase proteins and clinical responses post inoculation with immunogens indicating adverse pro-inflammatory reactions in mice in the present study.
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.
  Nagi A. Al-Haj , Lai L. Suang , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by Lipopolysaccharides (LPS), an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study was designed to further elucidate genes involved in the biosynthesis pathway of LPS in melioidosis agent followed with selected gene product expression with essential function for survival and virulence melioidosis agent. Expression of Bplps0013/lpxA and Bplps0007/rfaF successful expressed the entire proteins in 2 h with sizes of approximately 29 kDa and 43.7 kDa, respectively. The baseline information provided through the present research can be a preliminary approach towards the development of effective therapeutics against melioidosis.
  Nagi A. AL-Haj , E. Amghalia , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Methicillin-resistant Staphylococcus aureus strains have appeared in countries worldwide and continue to be one of the most common hospital pathogens and it has become increasingly prevalent in community-acquired infections and provided strong evidence for the independent origins of health care-associated Methicillin-resistant Staphylococcus aureus and community-acquired. It has been shown that methicillin-susceptible S. aureus strains become MRSA strains by the acquisition of a staphylococcal cassette chromosome mec element carrying the mecA gene, which is responsible for methicillin resistance and has become essential for the characterization of Staphylococcus aureus clones in epidemiological studies. The objective of this study to identify the staphylococcal cassette chromosome mec types of methicillin-resistant Staphylococcus aureus isolated from different Malaysian Hospitals. PCR amplification and sequencing analysis were performed to determine the SCCmec type of MRSA. The present research successfully established molecular characteristics of local MRSA contribute as initial database of these isolates in order to fully understand the epidemiology, microbiology and pathophysiology of these infections.
  Nagi A. AL-Haj , E. Amghalia , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  For centuries honey had a valued place in traditional medicine, being used in the treatment of wounds and diseases of the gut. The scientific community has now rekindled interest in the therapeutic use of honey in modern medicine and a number of published reports support its use in certain medical conditions, including burns and wounds. The aim of the present study to the effectiveness of the antimicrobial activity of honey against Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive Staphylococcus aureus (MSSA) isolates collected from various Malaysian hospitals. Thirty isolated of Staphylococcus aureus were found to be resistant to routinely used higher antibiotics. Using an agar incorporation technique the sensitivity of these strains to honey was tested by the method of minimum inhibitory concentration. All the tested strains of Staphylococcus aureus showed inhibition with honey at concentrations of 25 and 30%. The present study recommended that the multidrug-resistant Staphylococcus aureus infection particularly wound and burns honey may be useful for controlling infection.
 
 
 
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