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Articles by P. Hanachi
Total Records ( 9 ) for P. Hanachi
  P. Hanachi , S.H. Kua , R. Asmah , G. Motalleb and O. Fauziah
  Normal biochemical processes in human body may produce free radicals. These free radicals can, in turn, lead to oxidative stress related disease. This study examined the antioxidant activity of Berberis vulgaris fruit extract and its cytotoxic effect on human liver cancer cell line (HepG2).The antioxidant activity of Berberis vulgaris Fruit Extract (BFE) was assay by β-carotene bleaching and 1-diphenyl-2-picrylhydrazyl (DPPH) assay. The screening of cytotoxic effect was carried out by the microculture tetrazolium salt (MTT) assay on the human liver cancer cell line (HepG2). The BFE with concentration of 5-140 μg mL-1 was used. The control group cell was without any treatment. Intracellular Alkaline Phosphatase (ALP) activity is determined by p-nitrophenyl phosphate. The concentration of 5 μg mL-1 was chosen for this test. In β-carotene bleaching, ascorbic acid showed the mean total antioxidant activity of 96.16±5.09%, f ollowed by BHT (66.71±2.52) and BFE (59.91±8.64). In DPPH, the EC50 of ascorbic acid was 0.252±0.000 mg mL-1, BHT (0.612±0.009 mg mL-1) and BFE (0.685±0.033 mg mL-1). The IC50 of BFE was found 106.0±10.1 μg mL-1. Beside reduction in cell proliferation the crude extract was capable of enhacing the intracellular protein content in cell cancer line by one fourth while intracellular alkaline phosphatase activity increased by 7 fold. The results showed that processed commercial Berberis vulgaris exhibited antioxidant properties, has the ability of reducing cell viability and may had the potential of enhancing the ALP activity probably through structural changes.
  M.E.T. Manal , P. Hanachi , I. Patimah , I.A. Siddig and O. Fauziah
  This study evaluates the chemopreventive effect of an aqueous extract of fresh leaves of Azadarichta indica against liver cancer. Twenty male rats (Spraque dawley), 150-250 g, were acclimatized for 1 week before use. The rats were divided randomly into 4 groups; cancer control group (C), cancer treated with 5% Azadarichta indica extract group (CAI), normal control group (N) and normal treated with 5% Azadarichta indica extract group (NAI) and each group contains 5 rats. Rats in group C and CAI were induced cancer by intraperitoneal injection of 200 mg kg-1 diethyl nitrosamine (DEN) as hepatocarcinogenesis initiator and then followed by 2-acetylaminofluorene as promoter of hepatocarcinogenesis (0.02% in food) for 2 weeks. The rats were then left for 2 weeks. The rats in group N and NAI were injected once intraperitoneally with corn oil and act as control. The plant was fed orally to CAI and NAI groups. Serum concentration of alpha-fetoprotein (AFP) as liver tumor marker was measured. Glutathione S-transferase (GST) and glutathione peroxidase (GPx) were measured in the serum and liver cytosol. The results of this study revealed that there is a significant difference (p≤0.05) between C and CAI groups in serum alpha-fetoprotein and, in serum and liver cytosol levels of glutathione S-transferase and glutathione peroxidase. While there was no significant difference (p0.05) between NAI and N group in GST and GPx level. As a conclusion from this study that the consumption of 5% of NEEM leaves aqueous extract resulted in complete inhibition of chemically induced hepatocarcinogenesis in Spraque dawley rats.
  P. Hanachi , S. Golkho and M. Norrozi
  This study was done to identify the relation of demographic factors , Zn > 70 and < 70 mg dL-1 in pregnant women with, red and white blood cells count (RBC, WBC) in 22 weeks of gestation. The pregnant women selected from Reproductive Research Center, Imam Hospital in Tehran, Iran. The subjects selected were recruited into the study after obtaining informed consent. A questionnaire was filled by participant in this study. Sixty five pregnant women were randomly selected after analyze their serum Zn level, divided 2 group with Zn > 70 and < 70 mg dL-1 both group were consist from viewpoint of age, social economic situation and BMI. The blood serum samples were analyzed to assess zinc status and antioxidant and Hb, RBC, WBC. The results of this study revealed that, the pregnant women had mean age of 20.5 ±3.6, with first pregnancy 22.3 ±3.53 age, BMI 23.2 ± 5.52. About 32.6% of them had high school and 56.8% had diploma and only 10.6% had university education. However, none of the socio-demographic factors were significantly associated with the rate of Zn in 2 groups. The mean of zinc in group of Zn < 70 and Zn > 70 was 58.8 ±10.1 and 112.7 ±21.8 mg dL-1, respectively. There was significant (p<0.05) relation in total antioxidant and Zn level in both groups. Micronutrient interactions are particularly important on antioxidant during pregnancy. In view of this, there is need for proper, adequate and balanced micronutrient supplementation during pregnancy to affect a healthy outcome.
  H.M. Jalalian , L.A. Latiff , S.T. Syed Hassan , P. Hanachi and M. Othman
  The present study focuses on the role of information technology in blood safety policies. In order to reach to higher levels of blood safety we have to put our maximum force in the recruitment of safe blood donors via public education. Innovative use of mass media in recruitment of blood donors, especially the youth and those of higher education level and disseminating the basic information about blood donation can be our success key. There is an urgent need to find new ways of recruiting blood donors rather than traditional methods of donor education. Effectiveness of internet based educational intervention in other disciplines of health has already been proven; however, few researches exist concerning internet application for online recruitment of safe blood donors. Finally, internet users` population and blood donors are similar in many aspects of their socio-demographic determinants and it is reasonable to deduce that online education and dissemination of knowledge of safe blood donation can be considered an efficient modus of recruitment of voluntary non-remunerated blood donors.
  A.L. Latiffah and P. Hanachi
  This study aimed to determine the association between anthropometric measurement and dyslipidemia and their association with hypertension among older people. A cross sectional study was done among the residents in two old folks homes (Rumah Seri Kenangan Cheras and Little Sisters of the Poor) in urban Malaysia using a pre-tested guided questionnaire. Measurements taken were the blood pressure level, height and weight measurement and lipid profile. There were 92 respondents participated in the study. The prevalence of hypertension was 51.1%. The study found significant association between anthropometric measurement and hypertension although there was no difference in waist hip ratio measurement among hypertensive and normotensive individuals. Level of total cholesterol, LDL-C and triglycerides were significantly higher among hypertensive individuals compared to normotensive individuals. The prevalence rate of hypertension among residents in this study is higher compared to the national prevalence rate among the older adults. Appropriate invention programmes should be reinforced to reduce the complications of hypertension especially among institutionalized elderly.
  P. Hanachi and S. Golkho
  The purpose of this study was to determine the effect of soy phytoestrogens and exercise on lipid profiles and menopausal symptoms in menopausal women. In this study, 37 postmenopausal women (mean age = 52.2 ± 4.6 years) were randomly assigned to soymilk (n = 15), exercise+soymilk (n = 12) and control (n = 10) which were provided daily for 3 months. Subjects were given soymilk (Maxsoy, Co.) which contained 12.5 g of soy protein with Genistein, 13 mg and Daidzein, 4.13 mg day-1. Menopausal symptoms was measured using the Kupperman index, a standard measure of climacteric symptoms that has been validated in menopausal women at baseline and 3 months of daily use. Total Antioxidant Status (TAS) of serum was measured using the Ferric Reducing Ability of Plasma (FRAP) assay. Soymilk supplementation, Soymilk and moderate exercise significantly (p<0.05) improved TAC level. Vasomotor symptoms were improved (p<0.05) in soymilk consumption and moderate exercise and soymilk groups compared with control group. Vaginal problem, sexual symptoms and vasomotor significantly reduced (p<0.05) after soymilk consumption. The soymilk treatment and exercise+soymilk decreased total cholesterol and triglyceride levels compared with control group. This study suggests that soymilk isoflavones for 3 months treatment may be a safe and effective alternative therapy for menopausal symptoms and may offer a benefit to enhance antioxidant status to improve women health and quality of life.
  A.L. Latiffah , P. Hanachi and S. Khania
  The aim of this study was to determine the prevalence of hypertension and its associated risk factors among University Putra Malaysia (UPM) retirees in Klang Valley, Malaysia. This study was conducted among UPM retirees aged 50 years and above. Seventy-three respondents (males n = 62, females n = 11) were selected randomly and interviewed using a pre-designed questionnaire to collect information on the above variables. Blood pressure and anthropometric parameters measurement were done using standardized technique. Prevalence of hypertension was 32.88% in males and 36.36% in females, with the highest proportion among those aged 70 years and above (75%). Isolated systolic hypertension was detected in 50% hypertensive subjects. The mean systolic blood pressure was 133.73±16.35 mmHg with a significant difference observed in age group and various ethnics (p<0.05). The mean diastolic blood pressure was 81.99±9.46 mmHg. Among hypertensive subjects, 70.83% were aware of their hypertension and 82.35% (14) were currently under treatment. Among ever diagnosed hypertensive, 73.33% were currently under treatment and a mere 40.91% achieved blood pressure control. Factors found to be significantly associated with hypertension were literacy (χ2 = 16.51, df = 2, p<0.05) and previous job (χ2 = 8.82, df = 1, p<0.05). The prevalence of hypertension in this study was high. It is highly recommend that proper screening of blood pressure should be emphasized among the retirees to increase their awareness on the importance of blood pressure control and to improve lifestyle modification of hypertension.
  O. Fauziah , P. Hanachi , S. Yogespiriya and R. Asmah
  The effect of 5% w/v of Strobilanthes crispus (SC) extract and glycyrrhizin in diethylnitrosamine and acetylaminofluorene induced hepatocellular carcinoma, which is a vital mechanism in cancer treatment, was studied in male Sprague-Dawley rats. The obtained results have shown a significant, increase (p<0.05) of liver microsome Aniline Hydroxylase (AH) in cancer group rats after 12 weeks. Treatment with glycyrrhizin caused decrease in liver AH activity compared to control. Meanwhile, treatment with SC caused overall decrease in liver AH activity almost near to control groups. Meanwhile, microscopic observation of the lesion score during hepatocarcinogenesis revealed that cells of cancer group without treatment were severely necrotic at week 12. However, S. crispus treatment was reduced the severity in cancer group rats at week 12. The result also indicate that SC only ameliorated the cancer incidence in the liver, however did not fully recover the liver tumor similar to the normal cells. This might be due to short experimental duration.
  S. GolKhoo , A.R. Ahmadi , P. Hanachi , F. Barantalab and M. Vaziri
  The HPLC system separated completely isofavonoids such as daidzein (15.2 min) and genstein (17.3 min). Initially, the concentrations of major isoflavone Genistein and Daidzein in the tested soy milk were determined. Commercial soy milk samples were analyzed for isoflavones and two major isoflavones detected: genistein 25.86 (mg L-1) ±0.66 SD and daidzein 8.25 (mg L-1) ±1.13 SD. Concentrations of genstein in soy milk were higher than daidzein. The results obtained in this study can serve as a basis for estimating amount of soy milk can be consumed by people as related to its main isoflavone content.
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