Evaluation of Lesion Scoring and Aniline Hydroxylase Activity in Hepatocarcinogenesis Rats Treated with Strobilanthes crispus
The effect of 5% w/v of Strobilanthes crispus (SC) extract and glycyrrhizin in diethylnitrosamine and acetylaminofluorene induced hepatocellular carcinoma, which is a vital mechanism in cancer treatment, was studied in male Sprague-Dawley rats. The obtained results have shown a significant, increase (p<0.05) of liver microsome Aniline Hydroxylase (AH) in cancer group rats after 12 weeks. Treatment with glycyrrhizin caused decrease in liver AH activity compared to control. Meanwhile, treatment with SC caused overall decrease in liver AH activity almost near to control groups. Meanwhile, microscopic observation of the lesion score during hepatocarcinogenesis revealed that cells of cancer group without treatment were severely necrotic at week 12. However, S. crispus treatment was reduced the severity in cancer group rats at week 12. The result also indicate that SC only ameliorated the cancer incidence in the liver, however did not fully recover the liver tumor similar to the normal cells. This might be due to short experimental duration.
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