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Articles by Nuzulia Irawati
Total Records ( 2 ) for Nuzulia Irawati
  Nurhayati , Nuzulia Irawati , Eryati Darwin and Nur Indrawaty Lipoeto
  Background and Objective: Geohelminth is a common cause of chronic infections in humans. Worm infections have been shown to have a protective effect against some diseases. The effect is related to the ability of the worm to modulate the host immune response through Th2. The aim of study was to investigate the correlation between IL-10 levels and blood cholesterol and glucose in geohelminth positive human subjects with mature immune systems. Materials and Methods: Stool examination for geohelminth was done by direct method using iodine and by Kato Katz method. IL-10 levels were measured by indirect enzyme-linked immunosorbent assays (ELISA) method. Total plasma cholesterol levels were examined by CHOD-PAP method and fasting blood glucose was measured using hexokinase method. Results: All subjects infected with geohelminth classified as a mild infection. There was no correlation between IL-10 levels and EC/GC (p>0.05) but there was a negative correlation between IL-10 levels with TC levels (p<0.01) and between IL-10 levels and FBG levels (p<0.01). Conclusion: A negative correlation between IL-10, TC and FBG further strengthens support for the role of Th2 especially IL-10 to low cholesterol and blood glucose levels in geohelminth infections.
  Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi
  Aedes aegypti is the major vector of DHF virus in the tropical and subtropical. The DHF prevention depends on vector control because the vaccine is still in development. Microsatellite has become on effective marker to obtain information about genetic diversity and analyze the structure of genetic population. The aim of this study was to isolate and characterize A. aegypti microsatellite markers, the Dengue Hemorrhagic Fever (DHF) vector in West Sumatra. Sequences containing microsatellites were obtained by enrichment method. Stages of works were as follows: isolation of A. aegypti genomic DNA, restriction with enzymes, ligation with adapters and hybridization by using microsatellite motifs. Furthermore, the candidate fragment contained motifs cloned on plasmid pGEM T easy vector using E. coli DH 5α with blue-white colony screening. The results showed 46 clones were successfully extracted from a total of 152 clones and became microsatellite motifs with repetition: (GA)3, (CTA)3, (GA)3 (TAAG)3, (ACTT)3 (TC)3 (AC)3. Eight pairs of primer were successfully designed from sequences containing microsatellite motifs with feasible flanking regions. The primer evaluation used 32 DNA samples of A. aegypti from 8 cities (population) in West Sumatra. These markers have been successfully amplified 9-17 alleles with amplification products ranging from 129-306 bp, with a high degree of polymorphism. Aedes aegypti microsatellite markers obtained can be used to analyze the structure of genetic population of A. aegypti and the obtained results were the additional microsatellite markers type of A. aegypti than what had previously existed.
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