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Articles by Nicolae PACALA
Total Records ( 7 ) for Nicolae PACALA
  Dorel DRONCA , Nicolae PACALA , Ioan BENCSIK , Marian BURA , Teofil OROIAN , Vasile CIGHI , Mihaela IVANCIA , Gabi DUMITRESCU , Daniela GOINA , Adela MARCU , Ada CEAN and Liliana BOCA
  Non additive genetic component that is the base of a quantitative character has no predictability, this is the reason for which the present paper is proposing to appreciate the special combinatory capacity for average nests weight and average body mass of the descendants at the age of 21 days, in a specific crossing scheme combining three rabbit breeds common in Timis County breeders New Zealand White, Large Chinchilla and Californian. The data obtained, statistic presented confirm a good special combinative capacity for this character, reason for which the crossing scheme is recommended for producing meat individuals destined for slaughterhouses. This scheme provides an exploitation of the high frequency of the heterozygote at maternal forms and at the final hybrids.
  Ada CEAN , Nicolae PACALA , Alexandra BOLEMAN , Gabi DUMITRESCU and Dorel DRONCA
  In vitro embryo production is a biotechnology with great potential for acceleration of the genetic progress for animal species, but in the same time it’s an important tool for research. Although there is considerable progress in the improving this technique, there is still a difference between the morphology of in vitro and in vivo produced embryos. Because of this, scoring of in vitro obtained embryos is difficult, since the majority of the existent systems for scoring embryo quality are based on information obtained from morphological parameters from in vivo produced embryos (Erenus et al., 1991, Hnida C., et al., 2004). The aim of this paper was to obtain embryos by in vitro fertilization (IVF) and morphometric characterize them. For in vitro fertilization we used the protocol described by Nagy A. (2003). The morphometric parameters taken in consideration were: the zona pelucida thickness, inner diameter, outer diameter, inner and outer perimeter of the embryo. The measurements were made with Quick Photo Micro 2.2 software. The fecundation rate was assessed at 24 hours after fecundation (54.7%). All embryos and unfertilized oocytes were measured. For embryos pellucid zone thickness was 9.09±0.47 μm, for oocytes 8.02±2.25 μm; for embryos the inner diameter was 99.98±3.80 μm, for oocytes 99.34±1.76 μm; for embryos outer diameter 116.18±2.70 μm and for oocytes it was 114.38±2.46 μm; the inner perimeter for embryos was 314.2±3.12 μm and for oocytes it was 314±1.93 μm; the outer perimeter for embryos was 369.3±5.97 μm and for oocytes 362.33±3.93 μm. The data suggests an increase of the embryos dimension compared with oocytes that were not fertilized. It can also be noticed that for embryos the thickness of the pellucid zone was higher (9.09 μm) compared with the unfertilized oocytes (8.02 μm).
  Alexandra IVAN , Nicolae PACALA , Ada CEAN and Dorel DRONCA
  During the last few years our group has focus on assessing mammalian embryos viability in order to improve methods currently used in different assisted reproductive techniques: embryo transfer procedures, in vitro embryo production, cultivation, and cryopreservation techniques. Besides morphometric characterization of in vivo and in vitro produced embryos, after embryo recovery/fertilization and during the in vitro cultivation we also applied different intravital staining tests. Fluoresceine diacetate (FDA) is a non fluorescent analog of fluoresceine, which can easily penetrate the cell membrane of the living cells and under cellular esterase activity is hydrolyzed to fluoresceine, which emit green fluorescence while exposed to a fluorescence source of 494-518 nm wave length. The FDA metabolism depends on the esterase activity and at the same time on the plasmatic membrane integrity. Propidium iodide (Pi) poses the capacity to penetrate the cell membrane of the nonviable cells, binds inside the DNA molecules and show a red fluorescence while the cells are exposed to a fluorescence microscope at 515-560 nm (Lakowicz, 2006). 0.5 mg/ml FDA were used together with 0.1 mg/ml Pi to form a mixture that allows us to observe at the same time both living and death cells within the embryo. The embryos recovered at 48 hours after hCG administration, were evaluated by morphological and intravital staining tests. By morphological criteria 18% of the embryos were evaluated as poor and very poor quality while applying the FDA -Pi, 13.79% of the embryos were scored as no viable, differences being statistically significant. After quality scoring the embryos were cultivated for 72 hours in M16 culture media to study their future developmental capacity. After 24 hours of in vitro cultivation on M 16 media the differences between the proportions of embryos evolving to the morula stage were distinct significant (χ2 test, p≤0.01), 61.3% for the control group comparing to 38.7% of the FDA and Pi group. After 48 hours of cultivation, the percentage of embryos, which developed was more reduce compared to the retarded embryos (χ2 test, p≤0.001), only for the control group embryos evolved to the hatched blastocyst stage (13.33%). For the FDA-Pi experimental group, embryos evolved in a small proportion to the expand blastocyst FDA and Pi (4.34%). Embryos developing up to blastocyst stage, indicates their ability to metabolize the staining dyes (Fluorescein diacetate), but the high percentage of retarded embryos shows a decreasing until stopping of developmental capacity after staining.
  Dorel DRONCA , Nicolae PACALA , Ioan BENCSIK , Ioan OPREA , Teofil OROIAN , Vasile CIGHI , Mihaela IVANCIA , Gabi DUMITRESCU , Ada CEAN and Liliana BOCA
  Integration of the Romania in the EU is imposing a special attention to hunting animal populations. The aim of the present paper was to study the quantitative evolution of the hunting population for the 13 species, in the period 2006-2010, in the hunting terrain 15 Alios, from Forest Domain-Timisoara, with a total surface of 8464 ha. The conclusion was that the numeric evolution of the species studied is normal, keeping a balance in between populations as a conclusion of natural and artificial selection. It was also noticed that there is a good correlation between the numbers of individuals from the species studied and the biogenic capacity of the hunting fond studied.
  Dorel DRONCA , Nicolae PACALA , Ioan BENCSIK , Marcel MATIUTI , Teofil OROIAN , Vasile CIGHI , Razvan POPA , Mihaela IVANCIA , Ada CEAN , Silvia ERINA and Liliana BOCA
  Integration of the Romania in the UE is imposing a special attention to hunting animal populations. The aim of the present paper is to study the quantitative evolution of the hunting population for the 13th species, in the period 2006-2010, in the hunting terrain 59 Giarmata, from Forest Domain-Timisoara, with a total surface of 8417 ha. The results showed that from the 13th studied species only 7 were identified from which 5 species kept the effectives constant, fact that demonstrates the existence of a balance between the number of individuals and the biogenic capacity of this hunting terrain. In return the European hare (Lepus europaeus P.) and Common Pheasant (Phasianus colchicus L.) registered on the entire studied period a decrease of the effectives with 33.9% and 18% respectively.
  Dorel DRONCA , Nicolae PACALA , Ioan BENCSIK , Marcel MATIUTI , Teofil OROIAN , Vasile CIGHI , Razvan POPA , Ada CEAN , Daniela TOBA and Liliana BOCA
  Non additive genetic component that is the base of a quantitative character has no predictability, this is the reason for which the present paper is proposing to appreciate the special combinatory capacity for total gain and average daily gain recorded the finishing period (30-80 days), in a specific crossing scheme combining three rabbit breeds common in Timis County breeders New Zealand White, Large Chinchilla and Californian. The data obtained, were statistically analyzed and demonstrates a good special combinative capacity for these characters, reason for which the crossing scheme is recommended for producing of meat individuals destined for slaughterhouses. This scheme provides an exploitation of the high fervency of the heterozygote at maternal forms and at the final hybrids.
  Ileana MICLEA , Nicolae PACALA , Marius ZAHAN , Andrea HETTIG , Iulian ROMAN and Vasile MICLEA
  As defense against oxidative stress, living systems employ antioxidants that they either produce or take up from the environment. Among these α-tocopherol and ascorbic acid are some of the most important and have been shown to protect cell components against oxidation. The goal of this research was to establish whether supplementation with α-tocopherol or/and ascorbic acid could improve viability and maturation of porcine oocytes. Pig oocytes were cultured for 44-45 hours at 37°C in 5% CO2 atmosphere in M199 containing 20 μM α-tocopherol or/and 750 μM ascorbic acid. Afterwards, cumulus oophorus expansion was assessed and oocytes were denuded. Viability and the presence of the first polar body were assessed by fluorescent staining with 3’, 6’ fluorescein diacetate and Hoechst 33258. Differences between treatments were analyzed by the analysis of variance and interpreted using the LSD test. Supplementation with 750 μM ascorbic acid and the combination of α- tocopherol and ascorbic acid resulted in significantly greater (p<0.05) percentages of COCs that were scored as 3. Overall, cumulus expansion was promoted by the 750 μM ascorbic acid treatment. The percentages of oocytes with a visible first polar body were highest when ascorbic acid and the antioxidant mix were added to the maturation media. However, these supplementations had a negative effect on oocyte viability. This was maximal if 20 μM α-tocopherol was present the medium. The ch indicates that 20 μM α-tocopherol has a positive influence on preserving oocyte viability while 750 μM ascorbic acid and the antioxidant combination promote cumulus oophorus expansion and the formation of the first polar body.
 
 
 
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