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Bulletine of UASVM Animal Science and Biotechnologies
Year: 2010  |  Volume: 67  |  Issue: 1  |  Page No.: 484 - 484

Morphometric Characterization of Mouse Embryos Obtained by In Vitro Fertilization

Ada CEAN, Nicolae PACALA, Alexandra BOLEMAN, Gabi DUMITRESCU and Dorel DRONCA    

Abstract: In vitro embryo production is a biotechnology with great potential for acceleration of the genetic progress for animal species, but in the same time it’s an important tool for research. Although there is considerable progress in the improving this technique, there is still a difference between the morphology of in vitro and in vivo produced embryos. Because of this, scoring of in vitro obtained embryos is difficult, since the majority of the existent systems for scoring embryo quality are based on information obtained from morphological parameters from in vivo produced embryos (Erenus et al., 1991, Hnida C., et al., 2004). The aim of this paper was to obtain embryos by in vitro fertilization (IVF) and morphometric characterize them. For in vitro fertilization we used the protocol described by Nagy A. (2003). The morphometric parameters taken in consideration were: the zona pelucida thickness, inner diameter, outer diameter, inner and outer perimeter of the embryo. The measurements were made with Quick Photo Micro 2.2 software. The fecundation rate was assessed at 24 hours after fecundation (54.7%). All embryos and unfertilized oocytes were measured. For embryos pellucid zone thickness was 9.09±0.47 μm, for oocytes 8.02±2.25 μm; for embryos the inner diameter was 99.98±3.80 μm, for oocytes 99.34±1.76 μm; for embryos outer diameter 116.18±2.70 μm and for oocytes it was 114.38±2.46 μm; the inner perimeter for embryos was 314.2±3.12 μm and for oocytes it was 314±1.93 μm; the outer perimeter for embryos was 369.3±5.97 μm and for oocytes 362.33±3.93 μm. The data suggests an increase of the embryos dimension compared with oocytes that were not fertilized. It can also be noticed that for embryos the thickness of the pellucid zone was higher (9.09 μm) compared with the unfertilized oocytes (8.02 μm).

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