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Articles by N. Kataria
Total Records ( 10 ) for N. Kataria
  N. Kataria , A.K. Kataria , N. Pandey , R. Maan , A. Joshi , L.N. Sankhla , M. Chaturvedi , R. Pachauri and P.K. Pilania
  An investigation was carried out in 1235 male and female goats of Marwari breed belonging to arid tracts in India to find out the role of serum magnesium as a biomarker of oxidative stress. The samples were collected during moderate and hot ambiences from apparently healthy animals and from the animals having gastrointestinal parasites, Peste des Petits Ruminants (PPR), mange and those affected by drought. The mean value recorded during moderate ambience was considered as control. The mean value of serum magnesium during hot ambience of animals having gastrointestinal parasites, PPR, mange and those affected by drought were significantly (p≤0.05) lower than control mean value. Minimum value was observed in drought affected animals. The control mean value of serum magnesium was 1.24±0.003 m mol L-1 and the lowest value in drought affected animals was 0.49±0.002 m mol L-1. Simultaneously, serum catalase was also determined in each case to assess oxidative stress because it is considered as a potent marker of oxidative stress. Significantly higher (p≤0.05) values of serum catalase in each case revealed the presence of oxidative stress in animals. A concomitant decrease in serum magnesium reflected towards its antioxidant property. It was concluded that oxidative stress resulted in lowering in the serum magnesium levels however, impact varied. Low serum magnesium in gastrointestinal parasitised, PPR, mange and drought affected animals could be the outcome of oxidative stress to the animals and therefore it can be opined that dietary deficiency of magnesium is not the only reason behind the low magnesium levels but oxidative stress mechanism also play pivotal role. Serum magnesium can be used effectively as one of the biomarkers to assess oxidative stress.
  B.S. Saini , N. Kataria and L.N. Sankhala
  In the present investigation, the Extracellular Fluid (ECF) volume and serum electrolytes were determined in healthy adult female Marwari sheep during control, thirst, drinking and recovery periods. The control mean value of ECF volume was 9.187±0.332. Advancement in thirst period resulted in gradual deficit in ECF volume and on days 5 of thirst period 35.278% change was observed in ECF volume. Immediately after rehydration, replenishment was only 13.824%. Even on day 10 of recovery period, replenishment was not complete in ECF volume. The mean values of various serum electrolytes viz., calcium, phosphorus, magnesium, chloride and sodium increased significantly (p≤0.05) as the days progressed except potassium which decreased significantly (p≤0.05). On day 5 of thirst period, the values of calcium, phosphorus, magnesium, chloride and sodium were 37.892, 45.654, 61.687, 34.454, 21.805 and 24.428% higher, respectively than the corresponding mean value during control period. Drinking resulted in lowering of all the electrolytes than their respective day 5 thirst period values except potassium which showed an increase at hour 1/2 of drinking period. At 72 h of drinking period all the serum electrolytes showed non-significant (p>0.05) differences from respective control mean values. In present study no clinical signs of oedema, staggering or other illness was observed after thirst period and drinking periods. It was concluded that a thirst period of 5 days caused a state of dehydration resulting in water loss from ECF compartment. Owing to slow replenishment in the ECF volume, close monitoring of the dehydrated animals is essential to replenish fluid volume along with fluid therapy for a longer period.
  R. Maan , N. Kataria , P.K. Pilania , A. Sharma , S. Arora , A. Joshi , L.N. Sankhala , S.K. Sharma , N. Mohammad , P. Nathawat and A.K. Kataria
  Serum Superoxide Dismutase (SOD) profiling was carried out in the Marwari breed of sheep from arid tracts in India. Animals were categorized according to sex and age. Blood samples were collected during moderate, extreme hot and cold ambiences to harvest sera. Mean value of serum SOD during moderate ambience was 171.76±2.72 kU L-1. Moderate mean value was considered as control. The mean value of serum SOD was significantly (p≤0.05) higher during hot and cold ambiences as compared to control. Mean value was 2.57 times higher in hot ambience and 1.54 times higher in cold ambience as compared to control. The sex and age effects were significant (p≤0.05) in all ambiences. The mean values were significantly (p≤0.05) higher in male animals than female animals. In each ambience the age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2.5-4.5 years of age. Increased SOD activities during extreme ambiences indicated the development of oxidative stress. Present study generated data of SOD which can be used as reference values for future studies and for diagnostic purposes. The pattern of variation of SOD activity indicated modulation of adaptive mechanisms to protect the body from changes in ambient temperatures and on this basis use of antioxidants in the animals during changing environments is recommended.
  N. Kataria , Abhimanu , S. Arora , A. Sharma , R. Maan , S. Soren , A.K. Kataria , N. Mohammad , P.K. Pilania , L.N. Sankhala , M.K. Thori , J.S. Gaur and A. Meena
  A study was carried out in male and female calves of Rathi breed belonging to arid tracts from India to find out status of free radical scavenging enzymes during hot ambience. Blood samples were collected during moderate and hot ambiences to harvest sera. Maximum ambient temperature ranged from 44.5-46.5°C during hot ambience. All calves were managed in similar conditions and were apparently healthy. Free radical scavenging enzymes included Superoxide Dismutase (SOD), Glutathione Reductase (GR) and Catalase (CAT). The moderate (control) mean values of serum SOD, GR and CAT were 173.00±1.43, 11.00±0.09 and 74.80±1.00 kU L-1, respectively irrespective of gender and age. Similar pattern of significant change (p≤0.05) was observed by all the three enzymes in heat stressed calves being higher during hot ambience. The mean values were 2.67, 2 and 1.41 times higher, respectively in heat stressed calves during hot ambience. The sex and age effects were significant (p≤0.05) in moderate and hot ambiences for all the three enzymes. The mean values were significantly (p≤0.05) higher in male animals than female animals. In each ambience the age effect showed a significant (p≤0.05) increase in the mean values being highest in the calves of 6 months to 1 year of age. Results clearly indicated that status of free radical enzymes changed in heat stressed calves probably to scavenge higher number of free radicals formed during hot ambience. All the three enzymes used are important biomarkers of oxidative stress. Present study provided data which can be used as reference values to assess the extent of oxidative stress. This will help in timely protection of calves from ensuing health disorders.
  N. Kataria , J. Bargujar , P.K. Pilania , N. Arya , T. Bhati , N. Mohammed , P. Nathawat , M. Mathur , S. Choudhary , S. Asopa , Abhimanu and A.K. Kataria
  To evaluate stress and metabolic adjustments in buffaloes with Post Parturient Haemoglobinuria (PPH), certain serum enzymes viz. Gamma-Glutamayl Transferase (GGT), Glucose-6-Phosphatase (Glc-6-Pase), aldolase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, malate dehydrogenase, arginase and 5'nucleotidase enzymes were determined. Simultaneously haemoglobin and serum calcium, phosphorus, glucose and proteins were also determined. Blood samples were collected from adult female healthy and buffaloes with post parturient haemoglobinuria. In affected group the mean values of all the parameters were compared with respective healthy mean values. Significantly (p≤0.05) increased serum GGT activity in buffaloes with PPH indicated stress. Activities of the enzymes related to glycolysis and hexose monophosphate shunt were found to be decreased in affected animals. Activities of other enzymes like Glc-6-Pase, GDH, MDH, ARG and 5'NT were found increased significantly (p≤0.05) in the affected cases. This pattern was suggestive of metabolic adjustments where rate of glycolytic pathway decreased and rate of gluconeogenesis was observed to be increased in the affected cases. Correspondingly, serum glucose concentration of affected animals was significantly (p≤0.05) higher. Serum calcium showed non significant (p>0.05) difference whereas serum phosphorus, proteins and haemoglobin levels decreased significantly (p≤0.05) in affected cases. Decreased levels of phosphorus and disturbed calcium to phosphorus ratio suggested that phosphorous deficiency plays a key role in causing haemoglobinuria in buffaloes. Pattern of variation in the activities of the enzymes related to various metabolic processes revealed the metabolic adjustments. The data obtained in the present investigation support the concept that sustained PPH stress may represent an integrating mechanism underlying metabolic adjustments for homeostasis. Better understanding between PPH stress and metabolic homeostasis can be instrumental in the design of novel therapeutic strategies.
  A. Joshi , N. Kataria , S. Sharma , A. Sharma , S. Arora , Abhimanu , R. Maan , S. Asopa , N. Arya , S. Khan and A.K. Kataria
  Present investigation was launched to detect oxidative stress in pregnant and non-pregnant Murrah buffaloes during extreme ambiences. Serum oxidase and peroxidase profiling was used as a tool to detect oxidative stress. Blood samples of apparently healthy adult female Murrah buffaloes were collected to harvest sera during extreme hot cold and moderate ambiences. Moderate mean value of serum oxidase was 50.32±1.21 UL-1 and of serum peroxidase was 49.51±1.11 mUL-1. Extreme hot and cold ambiences resulted into a significant (p≤0.05) rise in the activities of serum oxidase and peroxidase. Animals were classified as non pregnant milch, pregnant milch and pregnant dry and activities of both the enzymes were compared. It was observed that mean values of both the enzymes in non-pregnant milch, pregnant milch and pregnant dry differed significantly (p≤0.05) from each other, respectively in all the ambiences. Non pregnant milch animals showed lowest activities whereas pregnant dry animals exhibited highest activities of both the enzymes. Multipara animals showed significantly (p≤0.05) higher activities than primipara. Serum oxidase and peroxidase are considered as important markers to assess oxidative stress. Increased activities of both the enzymes reflected the presence of oxidative stress in the buffaloes. It was concluded that extreme ambiences resulted into oxidative stress affecting the animals of all the physiological states. In each ambience the magnitude of increase was greater in pregnant dry animals. Trend was similar in all the ambiences but impact was maximum during hot ambience. Multipara animals showed greater changes than primipara. It can be stated that multipara and pregnant dry animals require proper care and supplementation of immuno-nutrients in order to protect them from the ensuing risk of oxidative stress. Further serum oxidase and peroxidase activities can be used effectively in detection of oxidative stress.
  R. Maan , N. Kataria , S. Sharma , S. Arora , A. Sharma , A. Joshi , N. Mohammad , P.K. Pilania and L.N. Sankhala
  Serum Monoamine Oxidase (MAO) activities were determined in the Marwari breed of sheep from arid tracts in India during extreme ambient temperature periods. Animals were categorised according to sex and age. Blood samples were collected during moderate, extreme hot and cold ambient temperatures to harvest sera. The overall mean value of MAO was 288.88±5.10 U L-1 which was obtained from 630 animals irrespective of sex and age. The range was 102-600 U L-1. Further mean values were determined according to ambient temperatures irrespective of sex and age. Moderate overall mean value was 162.14±4.61 U L-1 which was obtained from 210 animals during moderate ambience. The mean value of serum MAO was significantly (p≤0.05) higher during hot and cold ambient temperatures as compared to moderate mean value. The sex and age effects were significant (p≤0.05) in all ambient temperatures. The mean values were significantly (p≤0.05) higher in male animals than female animals. Age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2.5-4.5 years of age. The sex and age effects were significant (p≤0.05) in all ambient temperatures. Pattern of variations in serum MAO activities indicated that oxidative stress developed in sheep due to extreme ambient temperatures. Impact of hot ambient temperature was greater than cold ambient temperature.
  A. Sharma , N. Kataria , N. Arya , M. Mathur , R. Maan , A. Joshi , Abhimanu , L.N. Sankhala , T. Bhati and A.K. Kataria
  A study was conducted to assess modulations in gluconeogenesis in Marwari goat of both sexes ageing 6 months to 4.5 years during moderate, hot and cold environmental temperature periods. Liver samples were collected to determine Phosphoenolpyruvate Carboxykinase (PEPCK), Glucose-6-Phosphatase (Glc-6-Pase), Aspartate amino Transferase (AST) and Alanine Aminotransferase (ALT) enzymes. Moderate mean values of liver tissue PEPCK, Glc-6-Pase, AST and ALT were 562.00±7.25 U g-1, 160.00±4.25 mU g-1, 3.6±0.02 U g-1 and 2.6±0.01 U g-1, respectively. The mean values were significantly (p≤0.05) higher during hot and cold environmental temperatures in comparison to moderate mean value in each case. The sex and age effects were significant (p≤0.05) in all the environmental temperatures for all the four enzymes and pattern of variation was similar. The mean values were significantly (p≤0.05) higher in female animals than male animals. Age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 2-4.5 years of age. Variation in the activities of all the enzymes studied clearly indicated the greater effect of hot environmental temperature on the modulation of gluconeogenesis. Hot period related change was maximum in the activity of PEPCK (4.29 fold) followed by Glc-6-Pase (3.5 fold), ALT (2.92 fold) and AST (2.38 fold). PEPCK and Glc-6-Pase are important enzymes of gluconeogenesis. Enhanced activities of these two enzymes showed adaptive response to combat the stress of environmental temperature through increased glucose production.
  P.K. Pilania , S. Solanki , N. Mohammed , S. Asopa , R. Maan , A. Joshi , L.N. Sankhala , M. Mathur , M.K. Thori , J.S. Gaur , A. Meena and N. Kataria
  An investigation was carried out to assess oxidative stress in goats of Marwari breed affected from gastrointestinal parasitism and pneumonia belonging to farmers’ stock of arid tract of Rajasthan State, India. The animals were grouped into healthy and affected. All the blood samples were collected in sterile tubes with anticoagulants for erythrocyte separation and determination of erythrocytic Catalase (CAT) and Superoxide Dismutase (SOD) as biomarkers of oxidative stress. Activities of both the enzymes were significantly (p≤0.05) higher in affected animals in comparison to healthy lot. Erythrocytic catalase activity was 2.37 times higher in goats having gastrointestinal parasitism and 3.06 times higher in goats affected with pneumonia. Erythrocytic superoxide dismutase activity was 4.77 times higher in goats having gastrointestinal parasitism and 6.40 times higher in goats affected with pneumonia. It was observed that goats affected with pneumonia showed higher magnitude of enzyme activities in comparison to those having gastrointestinal parasites. Magnitude of change was greater in SOD activities than CAT activities. It can be concluded that gastrointestinal parasites and pneumonia served to produce oxidative stress in the goat. Catalase and superoxide dismutase are considered as potent biomarkers of oxidative stress. They are also named as antioxidant enzymes. Increased activities of both these enzymes signified the presence of oxidative stress in order to combat excessive production of free radicals. Antioxidant supplementation is recommended in cases affected with conditions like parasites and pneumonia to protect the animals from oxidative stress and ensuing damage to cellular machinery.
  N. Mohammed , S. Solanki , T. Bhati , P. Nathawat , S.K. Sharma , S. Kumar , A. Joshi , P.K. Pilania , L.N. Sankhala , M. Mathur , A. Ahuja , N. Kataria and A.K. Kataria
  The present study was carried out on 17 positive cases (horses) of colic. Two cases died during the treatment. Ten apparently healthy equines (horses) were also included in the study which constituted control group. Clinico-physiological observations included clinical signs, temperature, pulse, respiratory rate per min and colour of mucosa and were noted daily before start of treatment. Laboratory diagnosis of equine colic was done by determining haematological parameters. Blood samples from control group as well as equines suffering from colic were subjected to haematological examinations comprising of haemoglobin, packed cell volume, total erythrocyte count, total leucocyte count, differential leucocyte count and platelet count. There was great variation in duration and severity of clinical manifestations. In all the cases abdominal pain, loss of appetite, depression and change in colour of mucous membrane from light pink to dark pink was present. Pulse rate, respiration rate and capillary refill time were increased highly significantly (p<0.01) than the mean values from control group but temperature was within normal range and non-significantly (p>0.05) changed. In present study haemoglobin, PCV and TEC counts was increased significantly (p<0.05) on 1st day (before treatment) in comparison to healthy control group and after-treatment group. There was non significant (p>0.05) difference in their values between control group and after treatment group. Pattern of variation observed in the present study regarding haematological parameters of colicky cases can contribute in generating data for future studies and in laboratory diagnosis.
 
 
 
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