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Articles by M.A. Hossain
Total Records ( 10 ) for M.A. Hossain
  M.O. Raihan , A. Brishti , S.M. Tareq , M. Khalequeuzzaman , M.F. Islam and M.A. Hossain
  Cancer is recognized primarily as a disease of uncontrolled cell division. Hence, all efforts are directed toward the identification of antiproliferative compounds. Accordingly, regression of tumor size and increase of survival time has been recognized as the primary objective end point of effectiveness in preclinical and clinical testing for the discovery of a new anticancer drug. Dried roots from P. zeylanica were powdered and extracted with methanol. The root extract then at the dose of 20, 30 and 40 mg kg-1 day (i.p.) was evaluated for antiproliferative activity against Ehrlich Ascites Carcinoma (EAC) cells in swiss albino mice. The experimental parameters like tumor cell count, mean survival time and increase in life span were evaluated to assess antiproliferative activity. The extract was administered intrapretoneally for 14 consecutive days to EAC cell bearing group of mice. Bleomycin at the dose of 0.3 mg kg-1 (i.p.) was used as a positive control. It has been found that the root extract at the dose of 40 mg kg-1 day (i.p.) significantly (p<0.05) decreases tumor weight, increases life span and reduces tumor cell growth rate in comparison to those of EAC bearing mice receiving no extract (negative control) in a dose-dependant manner. In vitro antioxidant and cytotoxic activity of the same extract were also assessed to link the finding with the strong antiproliferative activity.
  R. Alam , M.Z. Karim , M. Fida Hasan , M.A. Hossain and A.B.M. Mafizur Rahman
  The study was conducted with 45 Sugarcane clones grown at zero-N and 120 kg N ha-1 levels in order to evaluate their possible BNF (biological nitrogen fixation) capability under field conditions. Field evaluation led to identification of some clones viz., I 153/94, Co 846, B 34-104 and Isd 28 that demonstrated considerable BNF capability. The yields of these clones at zero-N were 54.30, 61.48, 53.69 and 63.97 t ha-1 but at 120 kg N ha¯1 it was 59.72, 65.75, 57.15 and 70.59 t ha-1, respectively. The non BNF clones Isd 2/54 and Isd 18 showed poor yield at zero-N (31.38 and 28.17 t ha-1) while at 120 kg N ha-1 it was 54.61 and 61.58 t ha-1. The BNF capable clones performed almost equally well under both zero-N and 120kg N ha-1 as demonstrated through number of tiller (000 ha-1), number of millable cane (000 ha-1) and leaf nitrogen content at 120 and 200 days after plantation (DAP). Under aseptic culture condition, root extract and cane juice from the BNF- endowed clones showed the presence of gram negative bacterium that have been subjected to further studies.
  M.A. Hossain , A.F. Islam and P.A. Iji
  This study was undertaken to investigate the growth performance and energy utilization of broiler chickens fed on conventional diets, based on soybean or canola, with fishmeal (SBM50 and Can50, respectively) or on Vegetable Protein (VP) diets without fishmeal (SBM75 and Can75). Feed intake was highest (p<0.001) on the SBM50 and Can50 diets and lowest on SBM75 diet. Birds in the SBM50 and Can50 diet groups were heavier (p<0.001) than the SBM75 and Can75 diet groups. Birds on SBM50 and Can50 diets achieved superior Feed Conversion Ratio (FCR), while birds on Can75 diet were the poorest. Mortality of birds was unaffected (p>0.05) between dietary groups. The Apparent Metabolizable Energy (AME) intake on the SBM50 and Can50 diets was higher (p<0.001) than in the VP. Fat intake was highest (p<0.001) on Can50 diet group while protein intake was unaffected by treatment. Heat Production (HP) was identical, but net energy of production (NEp) was improved (p<0.05) in the birds on SBM50 and Can50. Whole body energy as well as fat (p<0.05) and protein contents were also increased (p<0.01) in the SBM50 and Can50 dietary groups. Birds on SBM50 and Can50 diet groups retained higher energy as fat (REf) (p<0.05) and as protein (REp) (p<0.01). The efficiencies of utilization of ME for energy (kRE), protein (kREp) and fat (kREf) retentions were unaffected. The results demonstrated that birds on the conventional diets (SBM50; Can 50) utilized energy better and as such grew faster than the birds on VP (SBM75; Can75) diets.
  R. Baksha , R. Alam , M.Z. Karim , S.K. Paul , M.A. Hossain , M.A.S. Miah and A.B.M.M. Rahman
  Multiple shoots were obtained from shoot tip explant of sugar-cane (Saccharum officinarum) cultured on MS medium supplemented with BAP (0.5-2.0 mg l-1), Kn (0.1-0.5 mg l-1) and IBA (0.1-0.5 mg l-1). Roots were induced in in vitro regenerated shoots on half MS medium supplemented with 5.0 mg l-1 NAA, IBA and IAA. Plant regeneration from shoot tip was the highest on MS medium supplemented with BAP 2.0 mg l-1 and IBA 0.5 mg l-1. The reported experimental findings present a method of plant regeneration of sugar-cane variety Isd 28 through shoot tip culture. The plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 70.
  M.A. Hossain , A.F. Islam and P.A. Iji
  A study was conducted to investigate the effects of dietary protein sources and microbial enzyme supplementation on amino acid and mineral digestibility, tissue protein contents and endogenous enzyme activities of broiler chickens. Ross 308 day-old male broiler chicks (n = 160) were assigned randomly to four dietary treatments, each replicated five times, eight chicks per replicate, in a 2 x 2 factorial design. Two basal diets were formulated with soybean (SBM) and canola (CM) meals at a ratio of 75:25, respectively, along with basal grains and fed to the birds as such or supplemented with enzymes from 1 to 35 days. Enzymes had a positive effect on the gross responses (feed intake, body weight and feed conversion ratio) of the broiler chickens at 35 days. However, feed intake was significantly different (p<0.001) between protein sources but this did not affect (p>0.05) body weight and feed conversion ratio (FCR) between 1 and 35 days. Tissue protein contents and endogenous enzyme activities at 21 days (except for maltase) were unaffected by dietary sources and microbial enzyme supplementation. The activity of maltase was higher (p<0.05) on CM diets than on SBM diets, but this effect was absent when diets were supplemented with enzymes. The digestibility of threonine and lysine, measured at 35 days, was significantly (p<0.01) higher on SBM diets than on CM diets. Similarly the digestibility of valine, isoleucine and leucine was also higher (p<0.05) on SBM diet than in CM diet. Addition of enzymes also significantly (p<0.05) increased the digestibility of histidine, threonine, lysine and isoleucine, but not the digestibility of other amino acids measured at this period (35d). Enzymes had no effect (p>0.05) on mineral digestibility of broilers over the test period. The digestibility of Cu, Zn and Mg was increased (p<0.05) on CM diet, whereas Ca digestibility was higher on SBM diet at 35 days. It can be concluded that the improved growth of the birds might be a result of increased digestibility of amino acids and increase in feed consumption.
  Z. Rehena , M.N. Islam and M.A. Hossain
  An assessment was conducted in Sirajganj district of Bangladesh to determine the pond fishery resources and sustainable development of fisheries. Researchers observed 123 (56%), 59 (27%) and 37 (17%) of ponds were culture, semi-culture and non-culture ponds and on the basis of the season 192 (88%) of perennial and 27 (12%) of seasonal ponds were found out of 219 during this study period. It was observed that the highest number of ponds 175 (80%) was personal pond which occupied by the single owners and 44 (20%) was leased in pond which privately occupied by the multiple ownership. The range of cost for lease was varied from Tk. 6,000-8,000 acre-1. The average pond fish production from 219 sampling ponds in Sirajganj district was 80.51 kg decimal-1. Therefore, by using sustainable development model, pond fisheries production can be increased to a considerable level and contributes to the national economy.
  M.A. Hossain , M.T. Hossain and N. Absar
  The lectin, MSL-1 was subjected to various chemical modifications in order to ascertain the amino acid residues responsible for their hemagglutinating activity. Modification of MSL-1 with acetic anhydride blocked nineteen amino groups and five tyrosine residues per molecule of lectin and decreased complete hemagglutinating activity. De-O-acetylation regenerated four of the tyrosine residues and resulted in a recovery of 80% activity. The presence of inhibitory saccharide galactose, a significant protecting effect was observed and only 1.49 tyrosine residues and fifteen amino groups were found to be modified with significant retention of hemagglutinating activity. The treatment of lectin with citraconic anhydride showed that nineteen amino were modified with the loss of 25-30% hemagglutinating activity. Modification of lectin with N-acetyl imidazole resulted in acetylation of fifteen amino groups and six tyrosine residues per molecule. De-O-actylation also regenerated 4.5 tyrosine residues with the retention of 75% of its hemagglutinating activity. When modification was conducted in the presence of galactose, about 3.5 tyrosine residues were protected from modification with 80% hemagglutinating activity. Successive addition of NBS to MSL-1 solution resulted in the modification of five tryptophan residues per molecule of lectin at pH 6, 5 and 4, respectively with the loss of 20-30% hemagglutinating activity. With DEPC at pH 7.2, eight histidine residues were modified and in the presence of inhibitory saccharide galactose, five histidine residues were protected from modification with the retention of 90% hemagglutinating activity. This was further confirmed from the finding that the activity was regenerated when His-modified MSL-1 was treated with hydroxylamine. The overall modification studies indicated that four tyrosine and five histidine residues were located at the saccharide-binding site of the lectin. However, modification of tryptophan and lysine had no effect on the hemagglutinating activity.
  B.C. Roy , M.A. Hossain and M.A.I. Khan
  The experiment was conducted to findout the transplanting effects on growth and yield of five modern varieties and to identify suitable varieties for late T. Aman season during 2000 and 2001. The varieties used were BR23 and BRRI dhan33, BRRI dhan37, 38 and 39. Results showed that the planting rice in the month of August was found to be very much risky due to tidal water pressure which mostly led to total crop damage. Early September was the suitable transplanting time of modern varieties for higher grain yield. The results indicated that the varieties like BR23, BRRI dhan 37 and 38 could easily be transplanted up to the 4th week of September with a reasonably higher yield of about 3 t ha 1 in the southern region (tidal non-saline area) of Bangladesh.
  M.A. Rahman , M.A.I. Khan , S.M. Jobair Hossain , M. S. Hossain , M.A. Hossain and S.S. Haque
  The chromosomes were identified individually and standard karyotype of Lycopersicon esculentum cv. Oxheart was formulated. Roots were collected from germinating seeds and were pre-treated in a saturated aqueous solution of monobromonaphthalene. After iron-alum mordanting, standard hematoxylin method was followed. 2n = 24 chromosomes were found. Karyotype analysis was carried out following a quantitative method using scatter diagram technique. Only two chromosomes were identified individually and the rest were morphologically characterized. One of the two identified chromosomes had mean total length 3.1μ and mean arm ratio 1.3 whereas, the other one had mean total length 2.5μ and mean arm ratio 2.0. The length of the prometaphase chromosomes ranged from 1.3 to 3.1μ. The karyotype of the studied tomato genotype consists of 8 metacentric and 4 sub-metacentric chromosomes. One pair of satellited chromosomes was also observed.
  M.A. Hossain , M.R. Akanda , M. Mostofa and M.A. Awal
  Background: Lead (Pb) is one of the toxic substances equally important like other toxic heavy metals. Lead (Pb) has extensive commercial and industrial use despite of public health hazard. Therefore, the work reported here was conducted to detect the therapeutic application of garlic (Allium sativum) on hematological parameters in lead-induced broiler chickens. Materials and Methods: Three hundred and fifty commercial broiler chickens were grouped into 5 such as T0, T1, T2, T3 and T4 consisting of 70 birds each where T0 served as control. T1 was provided with lead acetate at 100 mg kg-1 b.wt., T2 had 100 mg kg-1 lead acetate+1% garlic supplement, T3 was fed with 100 mg kg-1 lead acetate+2% garlic supplement and T4 had 100 mg kg-1 lead acetate+4% garlic supplement with the aim to determine the hematological changes in lead exposed chickens. Results: The analysis of variance in different groups were statistically significant (p<0.01). The mean values of erythrocyte, Hb and PCV values significantly reduced from 2.217±0.020-2.062±0.047, 7.697±0.247-6.172±0.198 and 25.183±0.8122-23.532±1.001, respectively in group T1. The ameliorating effects of garlic in heavy metal lead (Pb) revealed most significant (p<0.01) increased erythrocyte, hemoglobin and Packed Cell Volume (PCV) values from 2.16±0.034-2.46±0.077, 8.362±0.262-10.44±0.26 and 23.64±0.90-30.68±0.75 in group T3, respectively. Similarly Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC) were also resulted increased mean values from 121.12±5.28-131.50±3.72, 38.83±1.38-47.86±1.76 and 14.03±0.89-17.55±1.03, respectively in group (T3). Present study also revealed the increased values of leukocytes that might be attributed to the inflammatory process of leukocytes caused by lead (Pb). Significantly (p<0.01) decreased values of leukocytes were observed in group T3 compared to group T2 and T4 due to the ameliorative action of garlic in lead exposed chickens. Garlic in lead (Pb) exposed chickens could be considered as a potent inhibitor of lymphocytic proliferation that was evident in the present study by matured numbers of leukocytes. Conclusion: The consumption of certain percentages of garlic indicated that it might be capable of enhancing elimination of toxic effects on hematological changes in lead exposed chickens.
 
 
 
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