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Articles by M. Bernardo Filho
Total Records ( 6 ) for M. Bernardo Filho
  A.R. Souza , A. Silva , T. Freitas , R. Freitas , B. Mazziotti , M.L. Gomes , G.F. Dir and M. Bernardo Filho
  Although biological effects of ultrasound must be avoided for safe diagnostic applications, the ultrasound ability to disrupt cell membranes has attracted interest as a method to facilitate drug and gene delivery. It is concerned that many natural or synthetic medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Over the last few decades, application of ultrasound has been attempted to enhance transdermal transport of several drugs, a method referred to as phonophoresis. It has been related that natural and synthetic products in folk or clinical medicine have been widely used by human beings due to its curative properties. However, sometimes the biological effects of these products are not fully known. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural and synthetic products are capable of reducing the efficiency of this radiolabeling. The purpose of this study was to investigate the effects of phonophoresis on the radiolabeling of blood elements with 99mTc in an in vivo study. The animals of the therapeutical group under anesthesia with ether, the rats had been submitted to the therapy with ultrasound of high frequency (0.8 to 1Mhz) in continuous way, in the dose of 0.5 W/cm , during 3 min, with perpendicular and circular movements of 2.5 cm of diameter 2 on the back during 6 days. It was used as half of contact between the transducer and the skin the proper gel (diclofenac) for ultrasound. After that, samples (0.5mL) of blood were incubated with stannous chloride (SnCl ) 2 and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that in the treated group was observed a reduce on the radiolabeling in IF-P (74.74%? 1.35 to 62.47.26%? 5.35). In the light of the results we suggest that the phonophoresis allows the attainment of higher local concentrations of diclofenac may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process of plasma proteins.
  T. F. Silva , R. F. Silva , A.L.C. Silva , M. L. Gomes and M. Bernardo Filho
  Human beings have been widely used natural products as medicines. However, there are many reports about their undesirable toxicological effects. Chitosan is a kind of polysaccharide derived from the chitin (copolymer of "-(164)-D-glucosamine and $-(164)-N-acetil-D-glucosamine) that it is found in abundance in the nature, mainly crustaceans. It Had to the basic character, attributed to the presence of the grouping amine in the repeated units, and to its biodegradability, these two polymers come sufficiently interesting in scientists and technologists, who have discovered diverse applications, especially in the biomedical area. It is concerned that many natural remedies may contain potentially toxic ingredients and contaminants such as heavy metals. Red Blood Cells (RBC) and plasma proteins labeled with technetium-99 m (99 mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radiolabeling. The aim of this study was to assess the effect of a chitosan extract on the labeling of blood elements with 99 mTc. In the preparation of the extracts it was used 310mg of chitosan diluted in 10mL of saline solution (NaCl 0.9%). Samples (0.5 mL) of blood from Wistar rats were incubated with 0.1 mL of the extracts during 1 hour. After that, the samples were incubated with stannous chloride (SnCl ) and 99 mTc. The blood was centrifuged and Plasma (P) and RBC were isolated. P and RBC were also precipitated with Trichloroacetic acid (TCA) 5% and Soluble (S) and Insoluble (I) Fraction (F) was determined. The results have shown that the extract has not altered the radiolabeling . It was described that some extracts as Fucus vesiculosus, Paullinia cupana, Mentha crispa L were able to alter the radiolabeling of blood elements. In the light of the results obtained we suggest that the referred extract has an antioxidant properties.
  R. F. Silva , T. F. Silva , G. F. Dir? , M. L. Gomes , J. F. de Oliveira , E. A. C. Lima , R. L. Jales , M. T. J. A. Catanho and M. Bernardo Filho
  Medicinal herbs are widely used for the human beings, however, there are many reports about their undesirable toxicological effects. Preparations from Arnica (Arnica montana) flowers have been used in traditional medicine since a long time for the treatment of inflammatory diseases. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radiolabeling. The aim of this work was to assess the effect of an extract of Arnica (infusion) on the labeling of blood elements with 99mTc. In the preparation of the extract it was used 200mg of the leaves of Arnica in 10mL of saline solution (NaCl 0.9%). Samples (0.5mL) of blood from Wistar rats were incubated with 0.1 mL of the extract during 1 hour. After that, the samples were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the extract has reduced the radiolabeling in IF-P (from 75. 91%?3. 58 to 67.26%?7.44). It was described that some extracts as Fucus vesiculosus, Paullinia cupana, Mentha crispa L and Coffea arabica were able to alter the radiolabeling. In the light of the results obtained we suggest that the referred extract may alter the efficiency of labeling of IF-P due to its capacity (i) to oxidize the stannous ion, (ii) to complex with stannous and pertechnetate ions to form double salts, (iii) to compete by the same binding sites to pertechnetate ion or (iv) by the generations of reactive species of Oxygen with direct action on the labeling process.
  M.L. Gomes , E.A.C. Lima , S.M.S. Souza , L.C. Bernardo , M.E. Penas , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural or synthetic drugs can alter the bioavailability of radiopharmaceuticals used in diagnostic imaging in nuclear medicine. The knowledge of such altered bioavailability is important in making diagnostic of diseases and to try to understand biological effect of drugs in specific organs. The use of natural products has been increasing in the world. Echinacea is a natural product widely used for the treatment of respiratory tract infections. The aim of this study was to evaluate the effect of the echinacea on the bioavailability of the radiopharmaceutical 99mTc-sodium pertechnetate (Na99mTcO4) and on the mass of organs to try to verify possible undesirable actions of the studied natural product. An extract of Echinacea was daily administered by intragastric via into Wistar rats during 10 days. Na99mTcO4 (3.7MBq) was administered by ocular plexus via and 10 minutes after the animals were sacrificed. Blood and various organs were removed, their mass determined, their radioactivity counted in a well counter and the percentages of the injected dose per gram of organ (%ID/g) calculated. The results showed that in the treated animals: (i) the mass of the organs was not modified; (ii) the %ID/g decreased in lungs. The effect of echinacea on the Na99mTcO4 bioavailability in the lung was statistically significant (Student test, p<0.05) and it could be explained by the metabolization or therapeutic action of this herb in this organ.
  T. F. Silva , R. F. Silva , M. L. Gomes , J. F. de Oliveira , E. A. C. Lima , R. L. Jales , M.T. J. A. Catanho and M. Bernardo Filho
  Human beings have been widely used natural products as medicines. However, sometimes the biological effects of these products are not fully known. Sacred Bark (Rhamnus purshina) has been used as a folk medicine for treating constipation, inflammation, tumors and asthma. It is concerned that many natural remedies may contain potentially toxic ingredients and contaminants such as heavy metals. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radiolabeling. The aim of this work was to assess the effect of an extract of sacred bark on the labeling of blood elements with 99mTc. In this study it was analyzed the heated and no heated extract of sacred bark. In the preparation of the extracts it was used 200mg of sacred bark diluted in 10mL of saline solution (NaCl 0.9%). Samples (0.5mL) of blood from Wistar rats were incubated with 0.1 mL of the extracts during 1 hour. After that, the samples were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the heated extract has reduced the radiolabeling in BC (from from 94.76%? 0.66 to 89.80%? 4.23). It was described that some extracts as Fucus vesiculosus, Paullinia cupana, Mentha crispa L were able to alter the radiolabeling. In the light of the results obtained we suggest that the referred extract when it is heated may induce the generation of activity metabolites with oxidant properties and/or reactive species of Oxygen with direct action on the labeling process.
  G.F. Dire , E.A.C. Lima , M.L. Gomes , S. Moreno , M.V.C. Faria , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. It is concerned that many natural medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radio labeling. The aim of this work was to assess the oxidant or antioxidant action of the agents presents in the extract of Sechium edule verifying its effects on the labeling of blood elements with 99mTc and on the inhibition of the activity of acetyl cholinesterase (AchE). A freshly extract of Sechium edule was administered to Wistar rats during 15 and 60 days. After that, samples (0.5ml) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the referred extract was able to reduce the radio labeling in BC to 15 days of treatment (from 98.77%± 0.45 to 90.35%± 5.04) to 60 days of treatment (from 98.77%± 0.45 to 53.53%± 0.91), in IF-BC (from 90.36%± 2.34 to 21.20%± 2.36) and in IF-P to 15 days of treatment (77.20%± 2.19 to 53.26%± 6.69) to 60 days of treatment (77.20%± 2.19 to 11.01%±3.22). The analysis of toxicology was performed by the determination of acetyl cholinesterase activity. In the light of the results the referred extract has not shown inhibitory action due to the activity of the enzyme. In the light of the results obtained we suggest that the referred extract may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process.
 
 
 
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