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International Journal of Molecular Medicine and Advance Sciences

Year: 2005  |  Volume: 1  |  Issue: 1  |  Page No.: 17 - 21

Evaluation of the Biological Effects of a Sonophoresis on the Radiolabeling of Blood Elements with Technetium-99m: An In vivo Study

A.R. Souza, A. Silva, T. Freitas, R. Freitas, B. Mazziotti, M.L. Gomes, G.F. Dir and M. Bernardo Filho

Abstract

Although biological effects of ultrasound must be avoided for safe diagnostic applications, the ultrasound ability to disrupt cell membranes has attracted interest as a method to facilitate drug and gene delivery. It is concerned that many natural or synthetic medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Over the last few decades, application of ultrasound has been attempted to enhance transdermal transport of several drugs, a method referred to as phonophoresis. It has been related that natural and synthetic products in folk or clinical medicine have been widely used by human beings due to its curative properties. However, sometimes the biological effects of these products are not fully known. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural and synthetic products are capable of reducing the efficiency of this radiolabeling. The purpose of this study was to investigate the effects of phonophoresis on the radiolabeling of blood elements with 99mTc in an in vivo study. The animals of the therapeutical group under anesthesia with ether, the rats had been submitted to the therapy with ultrasound of high frequency (0.8 to 1Mhz) in continuous way, in the dose of 0.5 W/cm , during 3 min, with perpendicular and circular movements of 2.5 cm of diameter 2 on the back during 6 days. It was used as half of contact between the transducer and the skin the proper gel (diclofenac) for ultrasound. After that, samples (0.5mL) of blood were incubated with stannous chloride (SnCl ) 2 and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that in the treated group was observed a reduce on the radiolabeling in IF-P (74.74%? 1.35 to 62.47.26%? 5.35). In the light of the results we suggest that the phonophoresis allows the attainment of higher local concentrations of diclofenac may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process of plasma proteins.

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