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Articles by Jamsari
Total Records ( 6 ) for Jamsari
  Jamsari , Lily Syukriani , Hashlin Pascananda Utami , Friedrich Herberg , Wolfgang Nellen and Istino Ferita
  Artificial inoculation is an important method for understanding the plant-virus interaction. Currently available techniques for virus artificial infection are whitefly based inoculation, grafting, mechanical, agroinoculation and particle bombardment. In fact, almost all the available inoculation methods do not result in ideal efficiency nor practicality. The purpose of this study is to establish an injection based technique for routine artificial inoculation of Geminivirus particle into chilli pepper plant. The following treatments were applied in this study: Different ratios of extract-buffer (0, 20 and 60% w/v) and injection at four different positions (no inoculation, shoot, middle of main vein and petiol). The presence of Geminivirus particles in plants whether for inoculum source and infection effectivity was confirmed using the polymerase chain reaction. Some morphological observations were also applied in measurement of infection effectivity. Overall, the incidence of infection was 18% and among these, the average intensity of disease was 53% in the four week post inoculation. Geminivirus transmission by injection technique described in this study could be a simple and effective method for geminivirus artificial infection. However, accuracy, simplicity and uniformity have to be accommodated for further application of this technique.
  Jamsari , Istino Ferita , Lily Syukriani , Helni Lalan , Friedrich Herberg and Wolfgang Nellen
  Whiteflies have attracted intensive attention from many agriculturists due to their direct feeding and plant virus transmission. The economic losses depend on certain species population existing on the field during chilli-pepper cultivation. More over, biotype characteristic will determine the effectivity of applied pest management. Based on this, detailed information on whiteflies in chilli-pepper cultivation is very important. We applied mitochondria cytochrome oxidase unit I gene sequence for detecting species and biotype of whiteflies existing in West Sumatera. Two species were successfully identified i.e., Bemisia tabaci and Trialeourides vaporariorum that are dominant in this region. Distribution of both species are separated by altitude, where B. tabaci exist predominantly from low altitude to medium altitude, whereas in high altitude it is not common. On the other hand, T. vaporariorum distribute mainly in high altitude and not in medium altitude. Furthermore biotype determination on B. tabaci identified the existence of B biotype in all areas studied.
  Minda Azhar , Dessy Natalia , Sumaryati Syukur , Vovien and Jamsari
  Fructose and Fructo-Oligosaccharides (FOS) are derived more practical from the enzymatic hydrolysis reaction of inulin. In this study, the gene fragments that encodes inulin hydrolysis enzyme were isolated from genomic B. licheniformis by Polymerase Chain Reaction (PCR) technique using new primers designed on conserved domain in family GH32 enzymes. Gene fragments were cloned into pGEM-T vector with E. coli as host cells and determined the sequence of nucleotide bases. Size of the gene fragment have been found 539 bp using the DPE.slF and DPE.eR primer pair. The gene fragment encodes 179 amino acid residues of protein fragment. The protein fragment has high homology with levanase of Bacillus subtilis BSn5, levanase of Bacillus subtilis subsp. subtilis str 168, exoinulinase of Pseudomonas mucidolents, exoinulinase of Paenibacillus polymyxa, exoinulinase of Geobacillus stearothermophilus, exoinulinase of Paenibacillus Aloe sp-11 and exoinulinase of Paenibacillus polymyxa SC2. The homology were 97, 97, 62, 62, 61, 62 and 62%, respectively. The primers can use to isolate gene fragment that encodes inulin hydrolysis enzyme from the Bacillus genus.
  Firda Arlina , Hafl Abbas , Sarbaini Anwar and Jamsari
  Kokok Balenggek chicken is one of the rare indigenous chickens in Indonesia. They are unique as they produce a melodious song like crow. They have syllabic diversity, as each portion of the call can be composed with different pitches and vocalizations. The identification and characterizations of local adorn chicken is very important for animal conservation. A research was conducted in west Sumatera to identify the genetic variability of the external genetic characteristic of Kokok Balenggek chicken. This research was conducted in a limited area of Solok district West Sumatera, Indonesia. About 203 (111 males and 92 females) Kokok Balenggek chicken were observed. The varieties are based on base colour of feather, colour of the plumage, flick feather, pattern of feather, shank colour and comb types. The method were used to analyze the rate of purity and native gene, the frequency of autosomal and sex-linked of the genes, feather pattern, rate of introgression from the exotic breed of Rhode Island Red, White Leghorn and of Barred Plymouth Rock. The research showed that the highest controlling genes characteristic external of kokok Balenggek chicken are coloured (ii), wild type pattern (e+), plain feather (ss) golden flick feather (ss), yellow shank coloured (Id_) and single comb (pp). A constitution on external genetic characteristic of Kokok Balenggek is ii e+_bbssId_pp. while The purity gene of Kokok Balenggek chicken has only 45.30%. Its genetic introgression is affected by the exotic breed from Europa and America with genetic introgression value of 0.5470. According to the rate of heterozygosity value of kokok Balenggek chicken varied within 29.41%. In conclusion, the Kokok Balenggek chickens show heterogeneity in the external genetic. To complete a set of their characterization further studies are needed on their quantitative traits and the molecular composition.
  Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi
  Aedes aegypti is the major vector of DHF virus in the tropical and subtropical. The DHF prevention depends on vector control because the vaccine is still in development. Microsatellite has become on effective marker to obtain information about genetic diversity and analyze the structure of genetic population. The aim of this study was to isolate and characterize A. aegypti microsatellite markers, the Dengue Hemorrhagic Fever (DHF) vector in West Sumatra. Sequences containing microsatellites were obtained by enrichment method. Stages of works were as follows: isolation of A. aegypti genomic DNA, restriction with enzymes, ligation with adapters and hybridization by using microsatellite motifs. Furthermore, the candidate fragment contained motifs cloned on plasmid pGEM T easy vector using E. coli DH 5α with blue-white colony screening. The results showed 46 clones were successfully extracted from a total of 152 clones and became microsatellite motifs with repetition: (GA)3, (CTA)3, (GA)3 (TAAG)3, (ACTT)3 (TC)3 (AC)3. Eight pairs of primer were successfully designed from sequences containing microsatellite motifs with feasible flanking regions. The primer evaluation used 32 DNA samples of A. aegypti from 8 cities (population) in West Sumatra. These markers have been successfully amplified 9-17 alleles with amplification products ranging from 129-306 bp, with a high degree of polymorphism. Aedes aegypti microsatellite markers obtained can be used to analyze the structure of genetic population of A. aegypti and the obtained results were the additional microsatellite markers type of A. aegypti than what had previously existed.
  Vabera Maslami , Yetti Marlida , Mirnawati , Jamsari and Yuliaty Shafan Nur
  Background and Objective: Glutamate is a non-essential amino acid and it improves the perception of the taste umami and serves as a building block of protein and physiological functions of the body. Increased use of glutamate in animal feed causes glutamate to rise globally. The aim of this study was to obtain the optimal conditions for glutamate production by Lactobacillus plantarum VM. Materials and Methods: Lactobacillus plantarum VM (L. plantarum VM) is a lactic acid bacteria originating from Minangkabau fermented foods and produces glutamate. The increased production of glutamate from Lactobacillus plantarum VM can be achieved by improving the nutrition and the growth environment of the bacteria. This study was designed in the form of a laboratory experiment protocol and was repeated 3 times. The variables measured in this study were the medium pH, temperature, incubation time, carbon source and nitrogen source. Results: The results of this study showed an optimum 5.5 pH (161.519 mg L–1), incubation time (36 h), temperature (36°C) (350.001 mg L–1), 11% glucose (566,535 mg L–1) and 0.5% peptone (680.525 mg L–1). Conclusion: Optimization of the initial pH of the media, incubation time, temperature, source C and source N can increase glutamate production.
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