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Asian Journal of Agricultural Research
Year: 2015  |  Volume: 9  |  Issue: 1  |  Page No.: 23 - 32

Injection Technique Could as a New Promising Method for Artificial Infection of Geminivirus Particles in Chili Pepper (Capsicum annuum L.)

Jamsari, Lily Syukriani, Hashlin Pascananda Utami, Friedrich Herberg, Wolfgang Nellen and Istino Ferita    

Abstract: Artificial inoculation is an important method for understanding the plant-virus interaction. Currently available techniques for virus artificial infection are whitefly based inoculation, grafting, mechanical, agroinoculation and particle bombardment. In fact, almost all the available inoculation methods do not result in ideal efficiency nor practicality. The purpose of this study is to establish an injection based technique for routine artificial inoculation of Geminivirus particle into chilli pepper plant. The following treatments were applied in this study: Different ratios of extract-buffer (0, 20 and 60% w/v) and injection at four different positions (no inoculation, shoot, middle of main vein and petiol). The presence of Geminivirus particles in plants whether for inoculum source and infection effectivity was confirmed using the polymerase chain reaction. Some morphological observations were also applied in measurement of infection effectivity. Overall, the incidence of infection was 18% and among these, the average intensity of disease was 53% in the four week post inoculation. Geminivirus transmission by injection technique described in this study could be a simple and effective method for geminivirus artificial infection. However, accuracy, simplicity and uniformity have to be accommodated for further application of this technique.

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