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Articles by J. Jahim
Total Records ( 2 ) for J. Jahim
  S.J.H.M. Yusof , M.S. Takriff , A. Amir , H. Kadhum , A.W. Mohammad and J. Jahim
  The addition of organic acids to the growth medium has been shown to stimulate solvent production and protect against the degeneration of ABE-producing clostridia. The objective of this study is to demonstrate the effect of introduction of butyric acid in the fermentation media on the solvent production by C. acetobutylicum NCIMB 619. In this study, batch cultures were carried out in Reinforced Clostridial Media (RCM) containing 0.0015 and 2.0 g L-1 of butyric acid, simultaneously with a control media (without addition of butyric acid) at 37°C for 72 h. It was found that the presence of butyric acid during the early stage of the fermentation process favor the solvent yield to glucose utilization up to 71%. By adding butyric acid, the concentration of acetone and butanol generated was improved significantly. In the case of 2.0 g L-1 butyric acid addition, acetone and butanol production was increased approximately 5 and 1.6 folds, respectively compared to control culture. The result obtained in this study showed that the addition of butyric acid not only promoted solvent production, but also induced butanol production at the initial stage of fermentation by C. acetobutylicum NCIMB 619.
  W.M. Alalayah , M.S. Kalil , A.A.H. Kadhum , J. Jahim , A. Zaharim , N.M. Alauj and A. El-Shafie
  Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of μmax (maximum specific growth rate) and Ks (saturation constant) were 0.398 h-1 and 5.509 g L-1, respectively, using glucose as the substrate. The experimental substrate and biomass-concentration profiles were in good agreement with those obtained by the kinetic-model predictions. By varying the conditions of the initial substrate concentration (1-40 g L-1), reaction temperature (25-40°C) and initial medium pH (4-8), the model predicted a maximum hydrogen yield of 3.24 mol H2 (mol glucose)-1. The experimental data collected utilising this design was successfully fitted to a second-order polynomial model. An optimum operating condition of 10 g L-1 initial substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.
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