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Journal of Applied Sciences
Year: 2010  |  Volume: 10  |  Issue: 21  |  Page No.: 2709 - 2712

The Effect of Initial Butyric Acid Addition on ABE Fermentation by C. acetobutylicum NCIMB 619

S.J.H.M. Yusof, M.S. Takriff, A. Amir, H. Kadhum, A.W. Mohammad and J. Jahim    

Abstract: The addition of organic acids to the growth medium has been shown to stimulate solvent production and protect against the degeneration of ABE-producing clostridia. The objective of this study is to demonstrate the effect of introduction of butyric acid in the fermentation media on the solvent production by C. acetobutylicum NCIMB 619. In this study, batch cultures were carried out in Reinforced Clostridial Media (RCM) containing 0.0015 and 2.0 g L-1 of butyric acid, simultaneously with a control media (without addition of butyric acid) at 37°C for 72 h. It was found that the presence of butyric acid during the early stage of the fermentation process favor the solvent yield to glucose utilization up to 71%. By adding butyric acid, the concentration of acetone and butanol generated was improved significantly. In the case of 2.0 g L-1 butyric acid addition, acetone and butanol production was increased approximately 5 and 1.6 folds, respectively compared to control culture. The result obtained in this study showed that the addition of butyric acid not only promoted solvent production, but also induced butanol production at the initial stage of fermentation by C. acetobutylicum NCIMB 619.

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