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Articles by Indu Shukla
Total Records ( 2 ) for Indu Shukla
  Abdul Viqar Khan , Qamar Uddin Ahmed , Athar Ali Khan and Indu Shukla
  Cycas rumphii is traditionally used to cure wide variety of ailments including malignant ulcers, wounds healing, sore throats and tuberculosis showing its great potential as an antimicrobial agent. Hence, aim of the present study was to explore antibacterial potential of the leaves of C. rumphii Miq. Dried leaves were separately macerated with methanol and double distilled water at room temperature to obtain methanol and aqueous (AQ) extracts. The methanol extract was further successively extracted with light petroleum ether (LPE), benzene (BZ), ethyl acetate (EtOAc) and methanol (MeOH) on hot water bath to get respective extracts. These extracts were evaluated for their antibacterial efficacy at six different concentrations (500 μg, 1, 2, 5, 10 and 15 mg mL-1) against seven G+ve and eleven G–ve hospital isolated bacterial strains causing several tropical diseases using disc diffusion method. EtOAc and MeOH extracts showed maximum antibacterial activity against most of the bacteria taken into account. The most susceptible Gram positive bacteria was S. albus with inhibition zone of 22 mm while most susceptible Gram negative bacteria was S. boydii with 21 mm inhibition zone with EtOAc extract while the most resistant bacteria was gram negative S. typhi. Present findings are suggestive of antibacterial agents in the leaves of C. rumphii which can be used in future for formulation of broad spectrum herbal antibacterial products. Results of present study also support various traditional uses associated with this plant which were suggestive of possible antibacterial potential of this plant. Hence, EtOAc and MeOH extracts of the leaves of C. rumphii deserve further investigations.
  Fatima Khan , Indu Shukla , Meher Rizvi , Tariq Mansoor and S.C. Sharma
  Staphylococcus aureus is a causative agent of many types of diseases throughout the world. Staphylococcal infections are of particular concern because of the causative bacteria offering resistance to a wide range of commonly used antibiotics. The formation of biofilm is the hallmark characteristic of S. aureus infection. Biofilms constitute reservoir of pathogens and are associated with resistance to antimicrobial agents and chronic infections. In this study 262 clinical strains of S. aureus were screened by tissue culture plate method, tube method and congo red agar method for biofilm formation. Antimicrobial sensitivity testing of these strains was done by Kirby bauer disc diffusion method. Tissue culture plate method detected 38 (14.51%) isolates as strong biofilm producers, 132 (50.38%) as moderate biofilm producers and 92 (35.11%) strains as nonproducers of biofilm. The congo red agar method had a low sensitivity and specificity of 67.65 and 89.13%. The tube method correlated well with tissue culture plate method with a sensitivity and specificity of 99.40 and 95.78% but the interpretation are observer dependent. Biofilm production was higher in methicillin resistant strains as compared to the methicillin sensitive strains of S. aureus. Biofilm producers were found to be more resistant to almost all the groups of antibiotics.
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